Effects of feeding management and breed type on muscle chemical composition and relationships between carcass and muscle compositional traits in steers

peer-reviewedThere is little published information on the chemical composition of muscle from beef steers produced in Irish production systems. The objective of this study was to determine the effects of feeding management and breed type on m. longissimus chemical composition of steers, and to examine relationships between selected carcass traits and measures of carcass and muscle composition. A total of 117 steers (65 Friesians and 52 Charolais Friesians) were assigned on weight within breed type to a pre-experimental slaughter group and to one of 12 finishing groups (6 feeding treatments by 2 finishing periods). The 6 feeding treatments were: (1) silage only offered ad libitum (SO), (2) and (3) SO plus a low concentrate level, (4) and (5) SO plus a high concentrate level, (6) concentrates ad libitum. In Treatments 2 and 4, the silage and concentrates were offered separately whereas in Treatments 3 and 5 they were offered as a total mixed ration (TMR). The two finishing periods were 105 and 175 days. Mean low, high and ad libitum concentrate levels were proportionately 0.415, 0.732 and 0.927, respectively, of daily dry matter intake. Carcass weight, fat depth, fat proportion in the rib joint and m. longissimus lipid concentration all increased (P < 0.01) asymptotically with increasing concentrate level. Carcass fat class (P < 0.07), perinephric plus retroperitoneal fat weight (P < 0.001), fat depth (P < 0.06), fat proportion in the rib joint (P < 0.001) and m. longissimus lipid concentration (P < 0.001) were higher for Friesians than for Charolais crosses. Carcass weight increased (P < 0.001) with increased duration of the finishing period, as did carcass fat class (P < 0.06), fat proportion in the rib joint (P < 0.001) and m. longissimus lipid concentration (P < 0.001). Method of feeding had no effect on any of the traits measured

Comparison of pasture and concentrate finishing of Holstein Friesian, Aberdeen Angus × Holstein Friesian and Belgian Blue × Holstein Friesian steers

peer-reviewedCrossbreeding Holstein Friesian dairy cows with both early and late maturing beef breed bulls is common in Ireland. This study concerned the comparison of spring-born Holstein Friesian (HF), Aberdeen Angus × Holstein Friesian (AA) and Belgian Blue × Holstein Friesian (BB) steers slaughtered directly off pasture in the autumn or following a period of concentrate finishing indoors. Male calves (18 per breed type) were reared together until August of their second year when they were assigned to a 3 (breed type) × 3 (finishing strategy) factorial experiment. The three finishing strategies were (i) pasture only for 94 days to slaughter (PE), (ii) concentrate ad libitum indoors for 94 days to slaughter (CE), and (iii) pasture only for 94 days followed by concentrate ad libitum indoors for 98 days to slaughter (PC). For HF, AA, and BB, mean carcass weight, carcass conformation score and carcass fat score values were 275, 284 and 301 (s.e. 5.1) kg, 1.75, 2.42 and 2.89 (s.e. 0.11), and 2.48, 2.89 and 2.17 (s.e. 0.11), respectively. Pasture alone supported live-weight and carcass-weight gains of approximately 800 g/day and 400 g/day, respectively. Live-weight and carcass-weight gains on concentrate ad libitum were approximately 1400 and 870 g/day, respectively. For PE, CE and PC, mean carcass weight, carcass conformation score and carcass fat score values were 244, 287 and 329 (s.e. 5.1) kg, 1.81, 2.56 and 2.69 (s.e. 0.11), and 1.83, 2.71 and 3.01 (s.e. 0.11), respectively. It is concluded that none of the breed types reached an acceptable carcass weight on PE and only HF had acceptable carcass finish. All breed types were acceptably finished on both concentrate finishing strategies

Quality Meat Production from Beef Cattle During Winter Finishing.

End of Project ReportA series of experiments were carried out to examine the performance of the UK metabolisable energy (ME)/metabolisable protein (MP) system in an Irish context, and to determine the response in lean tissue growth to changes in the form of nutrients available for absorption from the intestine. In Experiment 1, the response of finishing continental heifers to an increase in MP supply was examined. It was demonstrated that this type of animal responded positively to an increase in MP supply in excess of requirements as presently estimated by the UK ME/MP system. Such an anomaly requires clarification. In Experiment 2, growth, digestibility and nitrogen retention in finishing continental steers offered ad libitum, concentrates based on barley and soyabean or on a mixture of industrial by-products were examined. The observed higher nitrogen retention in animals offered the by-product based ration suggested that there is opportunity to increase carcass protein content by judicious choice of feed ingredients. This suggestion was explored in Experiment 3. In Experiment 3, nitrogen retention and carcass composition were measured in sheep offered rations which resulted in different patterns of volatile fatty acid supply from the rumen. Nitrogen retention and the growth of carcass lean tissue were increased by the inclusion of sodium propionate in a starch-based ration but not in a fibre-based ration. The apparently contradictory effects of an increase in propionate supply by dietary means (starch vs fibrebased rations) or by addition of a salt of propionic acid suggests that the pattern, as well as the total supply of propionate is physiologically important in the growing ruminant. The endocrine mechanism of changes in carcass composition was also explored in this experiment. Differences in plasma concentrations of hormones which play a major role in the partition of absorbed nutrients towards muscle or adipose tissue suggests a role for the endocrine system in the regulation of growth, independent of energy intake. In Experiment 4, the effect of starch form and concentration (a dietary means of increasing propionate supply) in high concentrate rations on growth, efficiency and estimated lean content was examined in Friesian bulls. Supporting mechanistic measurements were made in Friesian steers fed the corresponding experimental rations. For optimum growth, ground starch should not exceed 210 g/kg of the ration. When included at approximately 300 g/kg, ground rather than rolled starch had a negative impact on growth. Coarse rations containing 300g or 480g starch/kg resulted in similar growth and efficiency. An increase in ground but not rolled starch concentration decreased the insulin response to a glucose challenge

The effect of cereal type and feeding frequency on intake, rumen fermentation, digestibility, growth and carcass traits of finishing steers offered a grass silage-based diet

peer-reviewedThe effect of concentrate cereal type (rolled barley-based v. rolled wheat-based) and concentrate feeding frequency (one 6 kg feed v. two 3 kg feeds per day) on intake, rumen fermentation, diet digestibility and performance of finishing steers offered grass silage to appetite was evaluated over four experiments using a total of 154 animals. Not all four feeding treatments were used in each of the four experiments. The duration of the growth measurement period was 152, 112, 111 and 113 days for experiments 1 to 4, respectively, after which all animals were slaughtered. Dietary dry matter (DM) intake and in vivo digestibility, final live weight, kill-out proportion, carcass weight, carcass conformation score, carcass fat score and daily liveweight and estimated carcass gain were not affected (P > 0.05) by cereal type or feeding frequency. Cereal type or feeding frequency had no effect (P > 0.05) on feed conversion efficiency (FCE) expressed as either live-weight or carcass gain per unit DM intake. Neither mean rumen fluid pH or concentrations of ammonia or L-lactate were influenced by cereal type or feeding frequency. The mean molar proportion of propionate was higher and that of butyrate lower (P < 0.05) with wheat than with barley. Estimated carcass weight gain and FCE to carcass were similar for wheat based and barley-based concentrate as a supplement to grass silage offered either as one feed or two equal feeds daily

Optimising The Response To Supplementary Concentrates By Beef Cattle In Winter

End of project reportConcentrates are a major component of feed costs in winter finishing of beef cattle. Two separate experiments were carried out to evaluate the response to increasing supplementary concentrate level with grass silage and the effects of feeding the silage and concentrates separately or as a total mixed ration (TMR). In experiment 1, a total of 117 finishing steers (initial live weight 538 kg, s.d. 35.5) were assigned to a preexperimental slaughter group of 9 animals and to 6 feeding treatments of 18 animals each. The feeding treatments were: 1) silage (SO) only offered ad libitum, 2) SO plus a low level of concentrates offered separately (LS), 3) SO plus a low level of concentrates offered as a TMR (LM), 4) SO plus a medium level of concentrates offered separately (MS), 5) SO plus a medium level of concentrates offered as a TMR (MM), and 6) concentrates ad libitum plus a restricted silage allowance (AL). Low and medium target concentrate levels were 3 and 6 kg dry matter (DM) per head daily. When silage and concentrates were fed separately, the daily concentrate allowance was given in one morning feed. The animals were individually fed for a mean period of 132 days. After slaughter, carcasses were weighed and graded and the ribs joint was dissected into its component tissues. Silage DM intake decreased but total DM intake increased with increasing concentrate level. Live weight gains for SO, LS, LM, MS, MM and AL were 0.34, 0.86, 0.86, 1.02, 1.00 and 1.12 (s.e. 0.064) kg/day, respectively. Corresponding carcass weight gains were 0.25, 0.58, 0.58, 0.71, 0.68 and 0.82 (s.e. 0.028) kg/day. All measures of fatness increased, ribs joint bone proportion decreased, and muscle proportion was not significantly affected by dietary concentrate level. There were no significant interactions between concentrate level and method of feeding. Compared with offering the feeds separately, feeding a TMR increased silage DM intake by proportionately 0.06 and total DM intake by proportionately 0.04. Otherwise, method of feeding had no significant effect on performance, slaughter or carcass traits. Mean rumen pH decreased while ammonia concentration tended to increase with increasing concentrate level. Total volatile fatty acids and the acetate to propionate ratio were lowest for SO. Method of feeding had no significant effect on rumen fermentation

A note on the effect of post-mortem maturation on colour of bovine Longissimus dorsi muscle

peer-reviewedFinancial support to P.G. Dunne was provided under the Walsh Fellowship programme of Teagasc.Fifteen heifers were housed and fed a concentrate diet while 54 counterparts grazed at pasture for 90 days at which stage six heifers from each group were slaughtered. The remaining animals in the pasture group were then housed and offered either: concentrate only; concentrate plus grass silage with silage accounting for either 20% or 50% of the total dry matter offered; or zero-grazed grass plus concentrate with grass accounting for 83% of the dry matter offered. Heifers (3/diet) were slaughtered 28, 56, 91 and 120 days thereafter. Colour characteristics of M. longissimus dorsi (LD) were measured at 48 h post mortem. The LD was then vacuum-packaged and stored at between 0 and 4 °C in darkness for 12 days, when colour characteristics were again measured. Maturation of LD resulted in meat that had higher redness values (‘a’ value; P<0.001) and a more intense red colour (higher ‘C’ value; P<0.001) at 14 days post mortem than at 2 days, regardless of diet/duration of feeding. Maturation also resulted in a brighter colour (higher ‘L’ value; P<0.001) but this difference was greatest when cattle were slaughtered the day-56 time point

Canonical discriminant analysis of the fatty acid profile of muscle to authenticate beef from grass-fed and other beef production systems: Model development and validation

Abstract The potential of diet-induced differences in the fatty acid profile of muscle to discriminate beef from different feeding systems and its potential use as an authentication tool was investigated. Three canonical discriminant models were built and validated using the fatty acid profile of beef from animals fed solely on pasture or cereal-based concentrates for 11 months or on various pasture/grass silage/concentrate combinations, including concentrates enriched with plant oils. Results indicated that models could successfully discriminate between grass-, partially grass- and concentrate-fed beef (accuracy = 99%) and between grass-fed beef and beef from animals supplemented with plant oils (accuracy = 96%). The approach also showed potential for distinguishing between beef from exclusively pasture-fed cattle and beef from cattle fed on pasture preceded by a period on ensiled grass (accuracy = 89%). Models were also applied to beef samples from 9 different countries. Of 97 international samples, including samples stated to be grass-fed, only 5% were incorrectly classified as Irish-grass-fed beef. These results suggested that the models captured traits in the fatty acid profile that are characteristic of Irish grass-fed beef and that this feature could be used for distinguishing Irish grass-fed beef from beef from other regions

Characterisation of feedstuffs for ruminants.

End of Project ReportA wide variety of feed ingredients are used in the manufacture of compound feeds in Ireland. Unprocessed feedstuffs vary from batch to batch due to differences caused by variety, soils, weather, etc. By -product feeds may also vary due to the processes from which they were produced. Accurate information on the nutritive value of feeds is essential for accurate ration formulation. A series of experiments was carried out to determine various nutritional characteristics of concentrate ingredients either locally produced or imported into Ireland. From these experiments it was concluded that : * The digestibility values of concentrate ingredients derived in maintenance-fed sheep are applicable to maintenance-fed cattle. * Feed is not utilised as efficiently when the level of feeding is increased from maintenance to 2 x maintenance. * The improved feed conversion efficiency in steers offered a restricted allowance of concentrates cannot be attributed to a difference in digestibility but can be attributed in part to a lower rate of fat deposition compared to steers offered ad libitum concentrates. * The residue after oil extraction from Camelina sativa could replace some imported protein-rich feedstuffs in ruminant rations but to fully achieve this potential, the residual oil content must be decreased. * For measurement of ruminal degradability of concentrate ingredients a wide range of forage to concentrate ratios and feeding levels can be used. * It is important to consider the actual outflow rate of nutrients from the rumen when measuring the feed value of individual concentrate ingredients as this can have an impact on the effective degradability and the relative nutritive values assigned to such ingredients. * Large variations in ruminal degradation occur within and among feeds. The ruminal degradability of different samples of any one feed should be measured to determine their true nutritive value for feeding ruminants. * Within most concentrate ingredients examined, the variation in small intestinal digestibility (SID) due to source indicates a range in the quantity of amino acids supplied to the animal for productive purposes. The more rapid and cost effective in vitro technique can be used to screen the SID of concentrate ingredients. * Target volatile fatty acid (VFA) concentrations and proportions may be produced by varying the proportions of the individual ingredients in a concentrate ration. * An in vitro procedure allowed VFA production to be measured across a large range of feeds under standardised conditions. * On average, 75% of gas produced during ruminal fermentation consists of carbon dioxide. The variation in methane production among individual concentrate ingredients provides an opportunity to formulate rations to minimize environmental pollution with methane.European Union Structural Funds (EAGGF

An examination of the molecular mechanisms controlling the tissue accumulation of conjugated linoleic acid (CLA) in cattle

End of project reportLong chain n-3 polyunsaturated fatty acids (n-3 PUFA) and conjugated linoleic acid (CLA) have demonstrable and potential human health benefits in terms of preventing cancer, diabetes, chronic inflammation, obesity and coronary heart disease. Supplementation of cattle diets with a blend of oils rich in n-3 PUFA and linoleic acid have a synergistic effect on the accumulation of ruminal and tissue concentrations of trans vaccenic acid (TVA), the main substrate for ?-9 desaturase which is responsible for de novo tissue synthesis of the cis 9, trans 11 isomer of CLA. This dietary strategy translates into increases in milk concentrations of CLA in dairy cows; however, concentrations in the muscle of beef animals have not always been increased. There is an apparent paradox in that n-3 PUFA supplementation enhances ruminal synthesis of trans-vaccenic acid (TVA), but then inhibits its conversion to CLA possibly through altering the activity of ?-9 desaturase. Recently, the promoter regions of the bovine ?- 9 desaturase gene has been isolated and analysed and has been shown to contain a conserved PUFA response region

Production and carcass traits of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais × Holstein-Friesian male cattle

peer-reviewedThe increased proportion of Holstein genetic material in the dairy herd has consequences for beef production in Ireland. A total of 72 spring-born male calves (24 Holsteins (HO), 24 Friesian (FR) and 24 Charolais × Holstein-Friesians (CH)) were reared from calfhood to slaughter. Calves were artificially reared indoors and spent their first summer at pasture following which they were assigned, on a breed basis, to a factorial combination of two production systems (intensive 19-month bull beef and extensive 25-month steer beef) and two slaughter weights (560 and 650 kg). After slaughter the pistola hind quarter was separated into fat, bone and muscle. Live-weight gain, carcass gain, kill-out proportion, carcass conformation and carcass fat scores were 830, 811 and 859 (s.e. 14.9) g/day, 540, 533, 585 (s.e. 7.7) g/day, 526, 538 and 561 (s.e. 3.0) g/kg, 1.51, 2.18 and 2.96 (s.e. 0.085), and 3.40, 4.25 and 4.06 (s.e. 0.104) for HO, FR and CH, respectively. Corresponding values for pistola weight as a proportion of carcass weight, pistola muscle proportion and pistola fat proportion were 458, 459 and 461 (s.e. 2.6) g/kg, 657, 645 and 667 (s.e. 3.7) g/kg, and 132, 161 and 145 (s.e. 4.1) g/kg. Compared with the intensive system, animals on the extensive system had a lower (P < 0.001) daily live-weight gain, kill-out proportion and a lower muscle proportion in the pistola. Increasing slaughter weight increased (P < 0.001) carcass weight and carcass fat score and reduced the proportion of muscle in the pistola. Allometric regression coefficients for pistola weight on side weight, and total bone, muscle and fat weights on pistola weight were 0.898, 0.755, 0.900 and 1.910 respectively. It is concluded that HO grew at least as fast as FR but had a lower killout proportion. Carcass conformation and fat scores were greater for FR than for HO and muscle proportion in the pistola was lower and total fat proportion was higher. Compared with FR, CH had heavier carcasses, a higher kill-out proportion and less fat and more muscle in the pistola