A PRELIMINARY STUDY OF THE PALEOLITHIC AND NEOLITHIC CONTRIBUTION THE EUROPEAN MTDNA FLOW IN SHAPING THE GENETIC STRUCTURE OF RECENT BOSNIAN POPULATION

The mitochondrial DNA (mtDNA) polymorphisms in Bosnian human population was analyzed by means of hypervariable segment I and II (HVSI and HVSII) sequencing and restriction fragment-length polymorphism analysis of the mtDNA coding region. The results suggest that shaping the genetic structure of recent Bosnian population likely to be affected by the expansion from the European glacial refuges area at the end of the Last Glacial Maximum (LGM), postglacial expansions from southwestern refuges of Europe, the Italian Peninsula and the dispersion in periods of more recent historical events, from the East European Plain. Especially interesting feature of the Neolithic expansion in this area is the ancient African/South Asian haplogroup N1a with the HVSI variant 16147G, which is almost absent in Europe. The haplotyps HVSI with variant 16147G suggest the colonization of the Northeast Bosnia region by Neolithic communities in the Early Neolithic period of expansion through Europe, as evidenced by the archaeological remains of the Starcevo culture

Molekularna identifikacija netuberkuloznih mikobakterija primjenom genotype Mycobacterium CM/AS testa

The study aims to determine the frequency and diversity of clinical isolates of nontuberculous mycobacteria (NTM). Of the 306 samples, the presence of NTM was confirmed in 65 (21.20%). The most frequently isolated species were M. fortuitum (30.3%), M. gordonae (24.2%), M. chelonae (15.2%), M. xenopi (7.6%), M. kansasii (4.5%), M. avium ssp. (3%), M. celatum (3%), M. mucogenicum (1.5%), M. peregrinum (1.5%), M. goodii (1.5%) and Mycobacterium sp. (7.6%). The determination of the local spectrum NTM is important because of the significant geographical variation. Although molecular testing is relatively expensive, they provide a rapid and accurate identification of different types of NTM.Istraživanje ima za cilj utvrditi učestalost i raznolikost kliničkih izolata netuberkuloznih mikobakterija (NTM). Od 306 uzoraka primjenom molekularnog testa prisustvo NTM je potvrđeno kod 65 (21,20%). Najčešće izolovane vrste su M. fortuitum (30,3%), M. gordonae (24,2%), M. chelonae (15,2%), M. xenopi (7,6%), M. kansasii (4,5%), M. avium ssp. (3%), M. celatum (3%), M. mucogenicum (1,5%), M. peregrinum (1,5%), M. goodii (1,5%) i Mycobacterium sp. (7,6%). Utvrđivanje lokalnog spektra NTM važno je zbog značajnih geografskih varijacija izolovanih vrsta. Iako su molekularna ispitivanja relativno skupa, ona imaju prednost što osiguravaju brzu i tačnu identifikaciju različitih vrsta NTM

Analysis of the mitochondrial CYTB gene sequence in human populations of northeastern Bosnia

This study offers the first report on variation sequence of the mitochondrial cytochrome b (MT-CYTB) gene in populations from Bosnia (northeastern Bosnia). This study was designed on the analysis of the genetic diversity of two populations of different cultural-anthropological and genetic origin, Roma population and native/non-Roma population. The main aim of our study was to estimate the usefulness of the CYTB sequence in the analysis of genetic categorization of different populations and intergroup diversity, as well as to provide some additional information on haplogroup-associated polymorphisms within the CYTB region in defining haplogroup status. Estimation of the genetic diversity was done using intra and intergroup genetic indices. The population-specific polymorphisms have been found in both categories of the populations. The results of the analysis of genetic differentiation show significant pairwise Fst differences between the Romani and native populations. Also, registered significant genetic differentiation is illustrated on the level of genetic variation between subpopulations of the Roma and non-Roma origin. The important result in our study is the confirmation of the significance of the triad of polymorphisms T14783C-G15043A-G15301A, indicating the influence of Asian component of the maternal gene pool on the genetic structure of the studied population of the Roma. Our data show that the haplogroup polymorphisms exist in the CYTB region and can provide useful information on the haplogroups that were defined only by the control region of the mtDNA. The results of this study indicate the region of CYTB gene can be a benefit in providing some additional information in the analysis of genetic structure of human populations and can be additionally applied in population studies