Limonium duriusculum (de Girard) Kuntze Exhibits Anti-inflammatory Effect Via NF-κB Pathway Modulation

HIGHLIGHTS L. duriusculum n-BuOH extract reduces inflammatory responses both in vitro and in vivo. L. duriusculum n-BuOH extract inhibits NF-κB-dependent transcriptional responses. L. duriusculum n-BuOH extract decreases the expression of TNF-α and IL-6 genes

Semi-groupes générés dans les espace Lp par un processus de dispersion incluant des conditions de semi-perméabilité à l'interface

We study an elliptic differential equation set in two habitats under semi-permeability conditions at the interface. This equation describes some dispersal process in population dynamics. Using functional calculus and results in Lutz Weis [22] among others, we show that the associated space operator generates an analytic semigroup in Lp-spaces.On étudie une équation différentielle elliptique posée dans deux habitats sous des conditions de semi-perméabilité à l'interface. Cette équation décrit un processus de dispersion en dynamique des populations. En utilisant le calcul fonctionnel et les résultats de Lutz Weis [22] entre autres, on montre que l'opérateur spatial associé génère un semi-groupe analytique dans les espaces Lp

Antioxidant and protective effect of Cynara cardunculus against paracetamol induced liver mitochondria oxidative stress.

Cynara cardunculus L. (Asteraceae), commonly known as cardoon, is a Mediterranean species that grows naturally in harsh habitat conditions. It is used as a food for its nutritional value and ethnomedicinal properties linked to liver cleansing. The aim of the present study was to evaluate the hepatoprotective action of Cynara cardunculus butanolic extract (CCBE) against paracetamol (APAP) induced acute liver injury in rats. Results showed that APAP intoxication caused increase levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). This phenomenon was paralleled by an impaired liver mitochondria redox status (reduced glutathione (GSH)), glutathione peroxidase (GPx), glutathione S-transferase (GST), and superoxide dismutase (SOD) and increased lipid peroxidation (LPO) in APAP treated rats. The pretreatment with CCBE blocked the increases of serum enzymes, reversed the mitochondrial LPO levels, and restored the mitochondrial antioxidant defense system (GSH, GST, GPX and SOD). The effect of CCBE was comparable to that of standard antioxidant N-acetylcysteine. Moreover, the antioxidant property of CCBE was also proved by in vitro assays as established by DPPH and hydroxyl radical scavenging activity and iron chelating ability. Our results indicate that the protective mechanism of CCBE may underlie the radical scavenging activity and the enhancement of mitochondrial antioxidant system.

Antioxidant and protective effect of Cynara cardunculus against paracetamol induced liver mitochondria oxidative stress.

Cynara cardunculus L. (Asteraceae), commonly known as cardoon, is a Mediterranean species that grows naturally in harsh habitat conditions. It is used as a food for its nutritional value and ethnomedicinal properties linked to liver cleansing. The aim of the present study was to evaluate the hepatoprotective action of Cynara cardunculus butanolic extract (CCBE) against paracetamol (APAP) induced acute liver injury in rats. Results showed that APAP intoxication caused increase levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). This phenomenon was paralleled by an impaired liver mitochondria redox status (reduced glutathione (GSH)), glutathione peroxidase (GPx), glutathione S-transferase (GST), and superoxide dismutase (SOD) and increased lipid peroxidation (LPO) in APAP treated rats. The pretreatment with CCBE blocked the increases of serum enzymes, reversed the mitochondrial LPO levels, and restored the mitochondrial antioxidant defense system (GSH, GST, GPX and SOD). The effect of CCBE was comparable to that of standard antioxidant N-acetylcysteine. Moreover, the antioxidant property of CCBE was also proved by in vitro assays as established by DPPH and hydroxyl radical scavenging activity and iron chelating ability. Our results indicate that the protective mechanism of CCBE may underlie the radical scavenging activity and the enhancement of mitochondrial antioxidant system.

Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds

Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds

HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts of the aerial parts of Genista ulicina Spach. from Algeria. The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and n-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc extract exhibited good antioxidant activities comparing to the n-BuOH extract. Thus Genista ulicina could be indicated as a plant of phytopharmaceutical importance