Influence of Trichoderma reesei or Saccharomyces cerevisiae on performance, ruminal fermentation, carcass characteristics and blood biochemistry of lambs fed Atriplex nummularia and Acacia saligna mixture

The mixtureof A.nummularia and A.saligna (1:1 DM)was autoclaved(TuttnauerUSACo.Ltd.,NY,USA)at121 °C and1.5psi for 15mintodestroyanymicrobes.Thecontentwasallowedto cool andlaterinoculatedwiththesporesof T.reesei at arateof 40 mLofthesporesuspensioncontaining107 spores permL/kg DM ofautoclaved A.nummularia and A.saligna mixture. Thein- oculatedsubstrateswerethenincubatedatambienttemperature for 10days.Bytheendoftheincubationperiod,theforageswere fullycoveredwiththefungus.Theywerethenovendriedat70 °C in aforcedairdryingoven(CascadeTEK’s ModelTFO-10,OR,USA) for 24hsoastostopfungalgrowthandpreventfurtherdena- turationofproteins.The aim of this study was to evaluate whole substitution of Egyptian berseem hay (Trifolium alexandrinum) with a mixture of Atriplex nummularia and Acacia saligna (1:1 DM) in the diet of Barki lambs for 70 days. Thirty six lambs (27.0±0.89 kg initial BW) were divided into four treatment groups of nine lambs each and fed: (1) the Control group with no substitution (70% concentrate mixture and 30% berseem hay, DM basis), (2) A. nummularia and A. saligna mixture without fungal treatment (treatment group AU), or (3) Trichoderma reesei treated A. nummularia and A. saligna mixture (treatment group AF), or (4) A. nummularia and A. saligna mixture supplemented with Saccharomyces cerevisiae at 0.5 g/kg DM of feed (treatment group AS) replaced 100% of berseem hay in the diet. Live-weight change, rumen fermentation parameters, blood chemistry, carcass characteristics and intestinal histology were investigated. Significant (P0.05). Lambs fed AF and AU diets had lower (P<0.05) feed conversion efficiency than lambs fed the AS and Control diets. Lambs fed AF and AS had increased (P<0.05) volatile fatty acid production compared to Controls. Blood albumin and urea concentrations increased (P<0.05) with lambs in AS treatment compared to lambs in the other treatments, while lambs fed AF had lower (P<0.05) cholesterol and glucose concentrations compared to the Controls. The AS lambs had the highest (P<0.05) dressing percentage. Decreased intramuscular fat weights were obtained with lambs fed halophytes compared to Control lambs. Histology of the ileum, sub mucosa and Peyer's patches were normal in all lambs. In conclusion, untreated halophyte mixtures of A. nummularia and A. saligna (at 1:1 DM) can be substituted for berseem hay without negative effects on performance while treatment with S. cerevisiae may improve performance and, like T. reesei, change certain biochemical responses

Orthogonal Sampling based Broad-Band Signal Generation with Low-Bandwidth Electronics

High-bandwidth signals are needed in many applications like radar, sensing, measurement and communications. Especially in optical networks, the sampling rate and analog bandwidth of digital-to-analog converters (DACs) is a bottleneck for further increasing data rates. To circumvent the sampling rate and bandwidth problem of electronic DACs, we demonstrate the generation of wide-band signals with low-bandwidth electronics. This generation is based on orthogonal sampling with sinc-pulse sequences in N parallel branches. The method not only reduces the sampling rate and bandwidth, at the same time the effective number of bits (ENOB) is improved, dramatically reducing the requirements on the electronic signal processing. In proof of concept experiments the generation of analog signals, as well as Nyquist shaped and normal data will be shown. In simulations we investigate the performance of 60 GHz data generation by 20 and 12 GHz electronics. The method can easily be integrated together with already existing electronic DAC designs and would be of great interest for all high-bandwidth applications

Potential impact of prickly pear cactus flour and Salix babylonica extract on cecal fermentation and methane production in horses

The cecal gas (GP) and methane (CH4) production and cecal fermentation kinetics when corn grain (CG) was replaced with prickly cactus (PC) in a horse’s diet at different levels of Salix babylonica (SB) extract was investigated. Three total mixed rations where CG was replaced with PC at three levels (/kg): 0 g (Control), 75 g (PC75) or 150 g (PC150) were prepared and SB extract added at four levels: 0, 0.6, 1.2 and 1.8 mL/g dry matter (DM) of substrates. No ration type 9 SB extract dose interaction was observed (P [0.05) for GP kinetics and CH4 production. Increasing the level of PC in the ration quadratically increased (P \0.01) the asymptotic GP and decreased (P\0.01) the rate and lag time of GP. Increasing the level of PC in the ration, increased GP values (P\0.05). Increasing the level of SB extract linearly decreased (P = 0.001) the lag time of GP of all diets without affecting the asymptotic GP or the rate of GP. Ration type and SB level had no effect (P [0.05) on CH4 production; however, at 36 h of incubation, SB extract decreased CH4 production. The rations PC75 and PC150 increased cecal pH compared with the control ration. The PC150 ration had the highest (P\0.05) DM degradability, short chain fatty acids production, and gas yield after 24 h of incubation, with no effect (P[0.05) of SB inclusion on all investigated fermentation kinetic parameters. It is concluded that increasing the level of PC in the diet of horse and replacing CG up to 60%, increased GP and improved cecal fermentation kinetics without affecting CH4 production. Inclusion of S. babylonica extract in the tested rations had weak effects on fermentation kinetics although it decreased the lag time of GP

Digestion, growth performance and caecal fermentation in growing rabbits fed diets containing foliage of browse trees

Feeding foliage from browse trees and shrubs may be of importance in animal production because these resources do not compete with human food and can provide significant amounts of nutrients. Rabbit is increasingly becoming an important meat source and its production is recommended in countries that are experiencing meat shortages, as it presents the best productive advantages to bridge the protein deficiency gap (Abdel-Aziz et al., 2015).This study aimed to evaluate the effect of feeding dried foliage (leaves and petioles) of Acacia saligna, Leucaena leucocephala or Moringa oleifera on the performance, digestibility, N utilisation, caecal fermentation and microbial profiles in New Zealand White (NZW) rabbits. One hundred weaned male NZW rabbits weighing 819.2±16.6 g and aged 35±1 d were randomly allocated into 4 groups of 25 rabbits each. Rabbits were fed on pelleted diets containing 70% concentrate mixture and 30% Egyptian berseem (Trifolium alexandrinum) hay (Control diet) or one of the other 3 experimental diets, where 50% of berseem hay was replaced with A. saligna (AS), L. leucocephala (LL) or M. oleifera (MO). Compared to Control diet, decreases in dry matter (DM; P=0.004), organic matter (P=0.028), crude protein (CP; P=0.001), neutral detergent fibre (P=0.033) and acid detergent fibre (P=0.011) digestibility were observed with the AS diet. However, DM and CP digestibility were increased by 3% with the MO diet, and N utilisation was decreased (P<0.05) with AS. Rabbits fed AS and LL diets showed decreased (P=0.001) average daily gain by 39 and 7%, respectively vs. Control. Feed conversion was similar in Control and MO rabbits, whereas rabbits fed AS diet ate up to 45% more feed (P=0.002) than Control rabbits to gain one kg of body weight. Caecal ammonia-N was increased (P=0.002) with LL, while acetic acid was decreased (P=0.001) with AS diet vs. other treatments. Caecal E. coli and Lactobacillus spp. bacteria counts were decreased with MO by about 44 and 51%, respectively, vs. Control. In conclusion, under the study conditions, tree foliage from M. oleifera and L. leucocephala are suitable fibrous ingredients to be included up to 150 g/kg in the diets of growing rabbits, and can safely replace 50% of berseem hay in diets of NZW rabbits without any adverse effect on their growth performance. Foliage from M. oleifera had a better potential as a feed for rabbits than that from L. leucocephala. Although foliage from A. saliga may be also used at 150 g/kg in the diets of growing rabbits, this level of inclusion may result in reduced feed digestibility and growth performance

Adsorption of Cd2+ and Cr3+ ions from aqueous solutions by using residue of Padina gymnospora waste as promising low-cost adsorbent

Recently, a great attention has been given for applying a low-cost and effective adsorbents instead of expensive and dangerous chemical materials as a promising approach to treat wastewater. In this work, residue powder of brown macroalga Padina gymnospora (RPG), after extracting most of its active components by 70% methanol, was used as an adsorbent material for wastewater treatment. This work also reduces the costs of residue disposal. The adsorption ability of RPG is studied for removing Cd2+ and Cr3+from wastewater. We investigated metal adsorption isotherms and kinetics, the effect of initial metal concentration, contact time, adsorbent dosage, temperature, pH and the RPG reusability on metal ions removal. The results showed that the removal % generally increases with decreasing concentration of metal ions. RPG has higher metal removal percentages reaching 96.2% and 78.8% for Cd2+ and Cr3+, respectively, with a maxiumum adsorption capacity of 96.46 and 31.52 mg/g for Cd2+and Cr3+,respectively at pH 6.2, 50 mg, 25 °C and initial metal concentration of 100 mg/L. The metal ions removal % increased by increasing the dosage of adsorbent and it decreased after a certain limit. The metal removal % slightly changes with increasing temperature for Cd2+ and decreased at high-temperature for Cr3+. The adsorption increased with increasing pH value from 3 to 5, and decreases at pH value of 6.2 then it increased again at pH 8. The removal % and adsorption capacity at pH 8 reaches 99.58%, 99.65%, 99.85 mg/g and 39.86 mg/g for Cd2+ and Cr3+, respectively. The results also showed that RPG can be reused several times for metal ions removal. In addition, Tempkin isotherms and pseudo-second-order kinetic fit the adsorption of Cd2+ and Cr3+ well

Growth performance and carcass characteristics of lambs fedhalophytes as a partial or whole replacement of berseem hay

our lambs per treatment were randomly chosen for blood collectionon days 35 and 70 of the experiment. About 10 mL of blood each lambwas collected from the jugular vein immediately before morning feeding.Collected blood was filled into a tube (BD Vacutainer®Tubes, NJ, USA)with heparin for anticoagulant and directly centrifuged at 4000 × g at 4◦Cfor 20 min. Plasma was separated into a glass vial and frozen at −20◦Cuntil analysis.tThe aim of this study was to evaluate partial or whole substitution of berseem hay withAtriplex nummularia (AT) and/or Acacia saligna (AC) in the diet of Barki lambs. Thirty-sixmale lambs (body weight 26.5 ± 1.1 kg and age 13 ± 1.1 months) were divided into fourgroups and fed: (1) the control diet (70% concentrate mixture and 30% berseem hay), (2)AT (AT15 diet) or (3) AC (AC15 diet) replaced 50% of berseem hay in the diet, or (4) ATand AC at a ratio of 1:1 to replace 100% of berseem hay (TC30 diet), respectively. Lambgrowth performance, rumen fermentation, blood chemistry, carcass characteristics andintestine histological properties were investigated. Dry matter intake, daily weight gainand feed conversion were measured every two weeks (period) for 10 weeks. Interactionswere observed (P < 0.05) between period × diet for these measurements and period affected(P < 0.05) daily weight gain and feed conversion. Lambs in the AT15 and AC15 groups hadincreased (P < 0.05) dry matter intake (g/lamb/day), water consumption (L/lamb/day), dailyweight gain (g/lamb/day) and feed conversion (feed intake/body weight gain) during thelast 2, 6, 2 and 8–10 weeks, respectively. The treatments AT15, AC15 and TC30 had decreasedconcentrations (P < 0.01) of ruminal volatile fatty acids, but did not affect ruminal ammonia-N concentrations. Lambs fed the experimental diets had lower (P < 0.05) concentrations ofblood cholesterol, low density lipoproteins and glucose and higher (P < 0.05) concentrationsof serum urea. The treatment AT15 had increased (P < 0.05) gut fill, pelt and mesenteryweights, whereas the treatment AC15 increased (P < 0.05) spleen and lung weight as a per-centage of carcass weight. In regards to the protein content of the 9–11th ribs, the treatmentAT15 was similar to the control, but the AC15 and TC30 were lower (P < 0.05) than the con-trol. Lambs in the treatment groups showed a normal histology of ileum, sub-mucosa andPeyer’s patches. It could be concluded that AT or AC can replace 50% of berseem in the dietof Barki lambs without compromising lamb growth performance