Influence of cellulase addition to dairy goat diets on digestion and fermentation, milk production and fatty acid content

Twenty four French Alpine goats (39 ± 2·0 kg) were individually housed in a completely randomized design and fed a basal diet containing 146 g crude protein and 356 g neutral detergent fibre (NDF)/kg in the absence (control – CTRL) or presence (CELL) of 2 ml of cellulase/kg dry matter intake (DMI) for 70 days, which included a 10-day adaptation period. The feed was offered three times daily at 07·00, 13·00 and 19·00 h, but the single daily dose of cellulase was only fed at 07·00 h. Goats were hand milked daily; milk production recorded and samples taken for compositional analysis. During the last 5 days of the experimental period, goats from each group were individually housed in stainless steel metabolic cages to enable separate and total collection of faeces and urine for nutrient digestibility and ruminal fermentation determinations. Goats fed CELL had greater DMI and greater digestibility of dry matter (DM), organic matter and NDF than CTRL goats. CELL goats had greater ruminal pH, concentration of acetic acid and concentration of propionic acid than CTRL goats. However, the concentration of ruminal butyric was lower in CELL goats compared with CTRL goats. CELL goats had greater milk yield, energy corrected milk, milk energy content, milk energy output and milk density than CTRL goats and the milk content for total solids, fat, protein and lactose were also greater for CELL goats than for the CTRL goats. The milk of CELL goats had greater palmitoleic acid, cis-10-heptadecanoic acid content and mono-saturated acids than the milk of CTRL goats and lower linoleic acid, linolenic acid contents and saturated fatty acids than the milk of CTRL goats. These results suggest that addition of 2 ml cellulase/kg DM of feed in the diet of lactating French Alpine goats elevated their milk production and improved its composition probably due to improved feed utilization.UAE

Lactation curves and body weight changes of Alpine, Saanen and Anglo-Nubian goats as well as pre-weaning growth of their kids

Thirty-six goats: 15 Alpine (AG), 13 Saanen (SG) and 8 Anglo-Nubian (ANG) were used to evaluate dry matter intake (DMI), body weight (BW) changes and milk production to validate Wood’s model in lactation curves. The kids pre weaning weight, weaning weight (WW) and daily weight gain (DWG) were determined. Milk production was measured daily for a period of 90 d. The DWG was quantified daily, while BW was recorded weekly. Performance of 48 kids (20 AG, 15 SG and 13 ANG), aged 15 d were evaluated. Wood’s model was utilised to describe goat’s lactation curve in the different studied goat breeds. Milk production was differed between breeds and according to the number of kids suckled (P < 0.05). Both of DMI and BW showed a linear increase (P < 0.05) during the studied period. Both of WW and DWG were unaffected by type of kidding and breed and were higher in males than in females. It is suggested that equation ofWood’s model is adequate to describe the lactation curve in studied goat breeds. The ANG and AG breeds versus SG had more persistence for milk production which was higher for those with single type of birth than those with twins.Universidad Autónoma del Estado de México, Universidad Autónoma Chaping

Forensic dissection of lip print as an investigative tool in a mixed Egyptian population

Background: Identification is a major problem facing forensic practitioners, DNA and finger prints are highly useful but sometimes aren’t easily collected from the crime scene. Lip print could be useful in this field being unique to each individual.Aim: The current study aimed at detecting the frequency and gender relation of lip print pattern in an Egyptian sample.Methodology: Samples were collected on white copy paper, divided into four quadrants then examined with magnifying lens for pattern distribution.Results: The study showed that pattern IV was the most frequently represented pattern in the study sample, pattern I &amp; II were more prevalent in males and females respectively. Prevalent pattern in Cairo and Lower Egypt was I while it was IV in Upper Egypt.Conclusion: The lip print pattern can differ due to gender and geographical origin in Egyptian subjects.Keywords: Lip print, Egypt, Gender, Geographical distribution, Identification, Anthropology. Gender differenc

Effect of glucoamylase enzyme extract on in vitro gas production and degradability of two diets with 25% of corn or sorghum grains

The optimal use of starch is fundamental in improving performance of ruminants fed high grain diets (Huntington 1997, Rojo et al. 2000). Many strategies were developed to increase starch digestion rate and grain energetic value such as ground, dry rolled and steamed, and harvest of grains with high moisture content (Owens et al. 1997). Amylolytic enzymes in the rumen are extracellular or cell-bound (Thurn and Kotarsky 1987), and the extracellular enzymes are the most important in the group of amylolytic bacteria (Cotta 1988). Amylases are present in protozoa (Mendoza et al. 1993 1995) and ruminal fungi (Yanke et al. 1993). Exogenous amylolytic enzymes are obtained from controlled fermentation of bacteria or fungi (Declerk et al. 1997) and they are used in the food industry for starch hydrolysis (Reilly 1985).The objective of this study was to evaluate the effect of glucoamylase enzyme (GEZ) extract on the in vitro ruminal gas production (GP) and degradability of 2 total mixed rations (TMR) of 25% of corn and other of 25% of sorghum grains. The 2 diets were treated with 0, 1.5 and 3 g of GEZ protein (65% of protein) per kg of grain in diet. Diets GP were measured at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation. Incubations were stopped after 72 h where pH was measured and supernatant was filtered to determine in vitro dry matter (DMD), neutral detergent fiber (NDFD), and acid detergent fiber (ADFD) degradabilities. Addition of GEZ to corn diet had no effect on kinetics of GP, whereas GEZ added to sorghum diet, at the high dose of the enzyme (3 g/kg DM), was traduced by an increase of the rhythm of GP (c) and the volume of GP at 2, 4 and 6 h of incubation. Likewise, effect of GEZ was not affected either on the DMD or cell wall (NDFD and ADFD) of both diets (sorghum or corn). Irrespective to enzyme supply, kinetics of GP and pattern of degradation of corn were generally higher than those of sorghum. A net effect of the diet and the interactions between diet and enzyme were recorded for the volume of GP at different incubation times. The use of high doses of GEZ should be tested on the pattern rumen fermentation

Influence of roasting, gamma ray irradiation and microwaving on ruminal dry matter and crude protein digestion of cottonseed

The aim of the current study was to compare the effect of different physical processing methods including roasting at 140 C for 15 (R15) or 30 minutes (R30), gamma ray irradiation (c-irradiation) at doses of 15 (c15), 30 (c30) and 45 (c45) kGy, and microwaving at 800 W for 2 (MW2), 4 (MW4) and 6 minutes (MW6) of whole cottonseed (WCS) on ruminal degradation. In vitro crude protein (CP) digestibility and gossypol contents were compared as well. In situ experiment was conducted on three permanent rumen-fistulated bulls. Gossypol content was decreased among treatments (p<0.05). The lowest degradation rate of protein in rumen was obtained for c45 treatment. The rate of degradation of the potentially degradation fraction was decreased for both dry matter (DM) (p¼0.002) and CP (p¼0.006) with different treatments. The lowest values for effective degradation in all passage rates were obtained with c45. Both microwaving and c irradiation showed difference for CP effective degradability parameter. The greatest value of in vitro CP digestibility (p<0.05) was observed for a dose of 45 kGy gamma-irradiated cottonseed compared to untreated WCS. Based on the results, c ray irradiation with 45 kGy was the most effective processing method in both reducing the gossypol content and escaping the protein through rumen for WCS in ruminant nutrition

Sustainable anaerobic rumen methane and carbon dioxide productions from prickly pear cactus flour by organic acid salts addition

Worldwide, agricultural byproducts produced during different agricultural practices are nutrients-rich feed ingredients with a large potential to be used for ruminants nutrition (Ahmed et al., 2015; Elghandour et al., 2016a); however, in many developing countries, agriculture byproducts always cause environmental problems resulting from their burning in the field. Such feeds can be used as a cleaner product of animal feed and environment (Elghandour et al., 2016b). Moreover, as the global population is increasing, the conventional feed for animal production, such as grains, legumes, etc. is in shortage and highly priced in many parts of the world.Ruminal fermentation is accompanied by production of methane (CH4) and carbon dioxide (CO2) which are greenhouses making the Earth warmer. Therefore, the sustainable production of CH4 and CO2 as well as fermentation kinetics when corn grain (CG) was replaced with prickly pear cactus flour (PC) was investigated. Besides, the effect of different levels of organic acid salts (OAS) was studied. Three total mixed rations used as substrates were prepared where CG was replaced with PC at three levels (/kg): 0 g (Control), 75 g (PC75) or 150 g (PC150). The OAS was used at three levels: 0, 5 and 10 mg/g dry matter (DM) of substrates. Asymptotic gas (GP), CH4, and CO2 productions as well as lag time had linear responses (P < 0.05) as PC level increased in the ration. Fractional rate of GP (P ¼ 0.007), GP, organic matter degradability, short chain fatty acid, and microbial biomass production (P < 0.001) were increased with increasing level of PC. Fermentation pH (P < 0.001), and DM degradability (P ¼ 0.0448) were linearly decreased as the PC level increased in the rations. Ration OAS interaction did not affect fermentation kinetics, GP, CH4 production and fermentation parameters. Prickly pear cactus flour at 150 g/kg DM inclusion level in ruminant ration has the potential to replace 60% of corn grain with enhanced fermentation and biodegradation efficiency in vitro; however, it increased CH4 production, which cannot be an environmental friendly way of feeding livestock

Influence of Trichoderma reesei or Saccharomyces cerevisiae on performance, ruminal fermentation, carcass characteristics and blood biochemistry of lambs fed Atriplex nummularia and Acacia saligna mixture

The mixtureof A.nummularia and A.saligna (1:1 DM)was autoclaved(TuttnauerUSACo.Ltd.,NY,USA)at121 °C and1.5psi for 15mintodestroyanymicrobes.Thecontentwasallowedto cool andlaterinoculatedwiththesporesof T.reesei at arateof 40 mLofthesporesuspensioncontaining107 spores permL/kg DM ofautoclaved A.nummularia and A.saligna mixture. Thein- oculatedsubstrateswerethenincubatedatambienttemperature for 10days.Bytheendoftheincubationperiod,theforageswere fullycoveredwiththefungus.Theywerethenovendriedat70 °C in aforcedairdryingoven(CascadeTEK’s ModelTFO-10,OR,USA) for 24hsoastostopfungalgrowthandpreventfurtherdena- turationofproteins.The aim of this study was to evaluate whole substitution of Egyptian berseem hay (Trifolium alexandrinum) with a mixture of Atriplex nummularia and Acacia saligna (1:1 DM) in the diet of Barki lambs for 70 days. Thirty six lambs (27.0±0.89 kg initial BW) were divided into four treatment groups of nine lambs each and fed: (1) the Control group with no substitution (70% concentrate mixture and 30% berseem hay, DM basis), (2) A. nummularia and A. saligna mixture without fungal treatment (treatment group AU), or (3) Trichoderma reesei treated A. nummularia and A. saligna mixture (treatment group AF), or (4) A. nummularia and A. saligna mixture supplemented with Saccharomyces cerevisiae at 0.5 g/kg DM of feed (treatment group AS) replaced 100% of berseem hay in the diet. Live-weight change, rumen fermentation parameters, blood chemistry, carcass characteristics and intestinal histology were investigated. Significant (P0.05). Lambs fed AF and AU diets had lower (P<0.05) feed conversion efficiency than lambs fed the AS and Control diets. Lambs fed AF and AS had increased (P<0.05) volatile fatty acid production compared to Controls. Blood albumin and urea concentrations increased (P<0.05) with lambs in AS treatment compared to lambs in the other treatments, while lambs fed AF had lower (P<0.05) cholesterol and glucose concentrations compared to the Controls. The AS lambs had the highest (P<0.05) dressing percentage. Decreased intramuscular fat weights were obtained with lambs fed halophytes compared to Control lambs. Histology of the ileum, sub mucosa and Peyer's patches were normal in all lambs. In conclusion, untreated halophyte mixtures of A. nummularia and A. saligna (at 1:1 DM) can be substituted for berseem hay without negative effects on performance while treatment with S. cerevisiae may improve performance and, like T. reesei, change certain biochemical responses

Chemical Composition and In Vitro Digestibility of Pleurotus ostreatus Spent Rice Straw

The nutritive value of rice straw (RS) and Pleurotus ostreatus spent rice straw (SRS) was studied by analyzing its proximate composition, fiber fractions, in vitro digestibility, amino acids content and scanning electron microscopy (SEM). The possibility of replacing berseem clover (BC; Trifolium alexandrinum) with SRS at different levels also was studied. Results showed higher protein content for SRS compared to RS (3.4 to 11.7%) while, DM, OM, NFE, CF, NDF, ADF, ADL, hemicellulose and cellulose were less for SRS than for RS. Highest concentration of amino acids (mg/100 g) was in SRS compared to RS. The SEM showed an extensive damage of SRS when compared to RS. Data also showed that SRS had higher in vitro dry matter disappearance (DMD) and in vitro organic matter disappearance (OMD) compared to RS. Results of in vitro study also, indicated that the levels of 50 and 90% replacement had the highest values of DMD and OMD compared to the other levels. It could be concluded from this study that treatment of RS with Pleurotus ostreatus improved the potential feeding value of the resultant substrates (i.e. SRS) as feed resources for ruminants and possibility of replacing BC with SRS at high levels of up to 50 or 90% from diets

The effects of three total mixed rations with different concentrate to maize silage ratios and different levels of microalgae Chlorella vulgaris on in vitro total gas, methane and carbon dioxide production

The aim of the current study was to assess the effects of adding Chlorella vulgaris algae at different levels on in vitro gas production (GP) of three total mixed rations (TMR) with different concentrate (C): maize silage (S) ratios (25C : 75S, 50C : 50S, 75C : 25S). Chlorella vulgaris was added at 0, 20, 40 and 80 mg/g dry matter (DM) of the TMR and total gas, methane (CH4) and carbon dioxide (CO2) production were recorded after 2, 4, 6, 8, 10, 12, 24 and 48 h of incubation in three runs. Increasing concentrate portion in the TMR linearly increased the asymptotic GP and decreased the rate of GP without affecting the lag time. Addition of C. vulgaris at 20 mg/ g DM to the 25C : 75S TMR increased the asymptotic GP, CH4, CO2 and GP at 48 h. Addition of C. vulgaris to the 50C : 50S TMR decreased the asymptotic GP and GP at 48 h. Higher CH4 production was observed at 48 h of incubation when C. vulgaris was included at (per g DM): 20 mg for the 25C : 75S ration, 40 mg for the 50C : 50S ration and 80 mg for the 75C : 25S ration. Inclusion of C. vulgaris linearly increased CH4 production for the 50C : 50S ration and increased CO2 production at 10 and 12 h of incubation for the 50C : 50S ration, whereas 20 and 40 mg C. vulgaris/g DM of the 75C : 25S TMR decreased CO2 production. The 25C : 75S TMR had the highest in vitro DM disappearance with C. vulgaris addition. Chlorella vulgaris addition was more effective with rations high in fibre content than those high in concentrates. It can be concluded that the optimal level of C. vulgaris addition was 20 mg/g DM for improved ruminal fermentation of the 25C : 75S TMR