CRISPR/Cas StNRL1 gene knockout increases resistance to late blight and susceptibility to early blight in potato

With the development of genome editing technologies, editing susceptible genes is a promising method to modify plants for resistance to stress. NPH3/RPT2-LIKE1 protein (NRL1) interacts with effector Pi02860 of Phytophthora infestans and creates a protein complex, promoting the proteasome-mediated degradation of the guanine nucleotide exchange factor SWAP70. SWAP70, as a positive regulator, enhances cell death triggered by the perception of the P. infestans pathogen-associated molecular pattern (PAMP) INF1. Using a clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a construct was made to introduce four guide RNAs into the potato cultivar Agria. A total of 60 putative transgenic lines were regenerated, in which 10 transgenic lines with deletions were selected and analyzed. A mutant line with a four-allelic knockdown of StNRL1 gene was obtained, showing an ~90% reduction in StNRL1 expression level, resulting in enhanced resistance to P. infestans. Surprisingly, mutant lines were susceptible to Alternaria alternata, suggesting that StNRL1 may play a role as a resistance gene; hence, silencing StNRL1 enhances resistance to P. infestans

In Vitro neurotoxicity and myotoxicity of Malaysian Naja sumatrana and Naja kaouthia venoms: Neutralization by monovalent and Neuro Polyvalent Antivenoms from Thailand

Naja sumatrana and Naja kaouthia are medically important elapids species found in Southeast Asia. Snake bite envenoming caused by these species may lead to morbidity or mortality if not treated with the appropriate antivenom. In this study, the in vitro neurotoxic and myotoxic effects N. sumatrana and N. kaouthia venoms from Malaysian specimens were assessed and compared. In addition, the neutralizing capability of Cobra Antivenom (CAV), King Cobra Antivenom (KCAV) and Neuro Polyvalent Antivenom (NPAV) from Thailand were compared. Both venoms produced concentration-dependent neurotoxic and myotoxic effects in the chick biventer cervicis nerve-muscle preparation. Based on the time to cause 90% inhibition of twitches (i.e. t90) N. kaouthia venom displayed more potent neurotoxic and myotoxic effects than N. sumatrana venom. All three of the antivenoms significantly attenuated venom-induced twitch reduction of indirectly stimulated tissues when added prior to venom. When added after N. sumatrana venom, at the t90 time point, CAV and NPAV partially restored the twitch height but has no significant effect on the reduction in twitch height caused by N. kaouthia venom. The addition of KCAV, at the t90 time point, did not reverse the attenuation of indirectly stimulated twitches caused by either venom. In addition, none of the antivenoms, when added prior to venom, prevented attenuation of directly stimulated twitches. Differences in the capability of antivenoms, especially NPAV and CAV, to reverse neurotoxicity and myotoxicity indicate that there is a need to isolate and characterize neurotoxins and myotoxins from Malaysian N. kaouthia and N. sumatrana venoms to improve neutralization capability of the antivenoms

The Mechanical Strength Properties, Treatability Retention and Hazard Classification of Treated Small-Clear Fast-Growing Acacia mangium Superbulk at Different Age Groups

The slow growth rate of primer species has affected the supply available to accommodate the market demand. To overcome these problems, a study was carried out to fully utilise fastgrowing timber as the primary resource to ensure the demand for timber logs continues. This paper aimed to determine mechanical strength properties, treatability retention, and hazard classification of 7-, 10- and 13-year-old small-clear samples of Acacia mangium superbulk collected from Daiken Plantation Sdn. Bhd. Bintulu, Sarawak, following treatment with 10% copper chrome arsenic. As a result of this study, the maximum strength was obtained from the 10-year-old age group, with the modulus of rupture (MOR), the modulus of elasticity (MOE), and compression parallel to the grain values of small-clear treated Acacia mangium superbulk reaching 118.76 N/mm2 , 15,020 N/mm2 , and 57.82 N/mm2 , respectively. In addition, the treatability retentions obtained were 149.27 L/m3 , 147.25 L/m3 , and 141.09 L/m3 , which were recorded from the 7-, 10-, and 13-year-old samples, respectively. Meanwhile, the dry-salt retentions obtained from the 7-, 10- and 13-year-old samples were 14.93 kg/m3 , 14.73 kg/m3 , and 14.11 kg/m3 , respectively. Hence, this species is classified as moderately difficult to treat under CCA (treatability retention: 80–160 L/m3 ) and is categorised under the hazard class of H4 (dry salt retention: 12–16 kg/m3 )

Physical and Mechanical Properties Performance between Untreated and Treated with CCA Treatment at Different Age Groups of Fast-Growing Acacia Hybrid of Sarawak

An effort was carried out to fully utilise fast-growing Acacia hybrid usage in the timber engineering field; however, the research data are still lacking. This paper aims to evaluate the physical and mechanical properties performance between untreated (control) and treated with 10% copper chrome arsenic of Acacia hybrid collected from Daikin Plantation Sdn. Bhd. Bintulu, Sarawak at air-dry condition at different age groups using the small clear method. Mechanical properties test refers to shear parallel to grain (tangential and radial directions), cleavage (tangential and radial directions), and tension parallel to grain test. Meanwhile, the physical properties test refers to moisture content (MC) and density test. After treatment, mechanical properties increase with an average of 13.67%; meanwhile, moisture content decreased with an average of 0.58% or 0.09% MC, and density slightly increased with an average of 0.44% or 0.002 g/cm3 . Results indicate that 10-yearold Acacia hybrid exhibits the highest strength values in shear parallel to the grain, tension parallel to the grain, and cleavage, followed by 13-year-old and 7-year-old. Treated samples in the tangential direction performed better with consistent mean results than that of the untreated samples, while radial direction gave a high average strength increment when treated

Physical and mechanical properties performance between untreated and treated with CCA treatment at different age groups of fast-growing acacia hybrid of Sarawak

An effort was carried out to fully utilise fast-growing Acacia hybrid usage in the timber engineering field; however, the research data are still lacking. This paper aims to evaluate the physical and mechanical properties performance between untreated (control) and treated with 10% copper chrome arsenic of Acacia hybrid collected from Daikin Plantation Sdn. Bhd. Bintulu, Sarawak at air-dry condition at different age groups using the small clear method. Mechanical properties test refers to shear parallel to grain (tangential and radial directions), cleavage (tangential and radial directions), and tension parallel to grain test. Meanwhile, the physical properties test refers to moisture content (MC) and density test. After treatment, mechanical properties increase with an average of 13.67%; meanwhile, moisture content decreased with an average of 0.58% or 0.09% MC, and density slightly increased with an average of 0.44% or 0.002 g/cm3. Results indicate that 10-yearold Acacia hybrid exhibits the highest strength values in shear parallel to the grain, tension parallel to the grain, and cleavage, followed by 13-year-old and 7-year-old. Treated samples in the tangential direction performed better with consistent mean results than that of the untreated samples, while radial direction gave a high average strength increment when treated

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Use of intron-exonic marker in assessment of genetic diversity of two subspecies of Thymus daenensis

Study of genetic diversity in medicinal plant is very important for improvement and evolutionary variations. In this study, assessment of genetic diversity in two subspecies of Thymus daenensis was evaluated, using intron-exonic markers. Thirty primers produced 633 polymorphic bands (98% polymorphism). The highest polymorphic information content (PIC) included ISJ5 and ISJ9 primers and the lowest PIC also included IT15-32 primer. The highest marker index (MI) produced by IT10-6 primer. Results of Analysis of Molecular Variance (AMOVA) showed that intra-sub specific variation was more than inter-sub specific variation. Dendrogram obtained from Cluster analysis, using NTSYS-pc software and UPGMA method based on Dice's similarity matrix, divided accessions into 4 groups. The maximum range of genetic similarity was observed between two accessions of sub-species daenensis. Two accessions of Fars and Semnan formed a separate group. Results showed that clustering based on molecular data and principal coordinate analysis had a medium alignment. Grouping based on cluster analysis also could separate two subspecies of Thymus daenensis. Results obtained from this study showed that intron-exonic markers had an effective potential in assessment of genetic relationships between the two sub-species of daenensis

Genetic diversity of different accessions of Thymus kotschyanus using RAPD marker

Analysis of genetic diversity is a major step for understanding evolution and breeding applications. Recent advances in the application of the polymerase chain reaction make it possible to score individuals at a large number of loci. The RAPD technique has been successfully used in a variety of taxonomic and genetic diversity studies. The genetic diversity of 18 accessions of Thymus kotschyanus collected from different districts of Iran has been reported in this study, using 30 random amplified polymorphic DNA primers. Multivariate statistical analyses including principal coordinate analysis (PCOA) and cluster analysis were used to group the accessions. From 29 primers, 385 bands were scored corresponding to an average of 13.27 bands per primer with 298 bands showing polymorphism (77.40%). A dendrogram constructed based on the UPGMA clustering method revealed three major clusters. The obtained results from grouping 18 accessions of T. kotschyanus with two studied methods indicated that in the most cases the applied methods produced similar grouping results. This study revealed nearly rich genetic diversity among T. kotschyanus accessions from different regions of Iran. The results showed RAPD marker was a useful marker for genetic diversity studies of T. kotschyanus and it was indicative of geographica variations

Isolation and identification of probiotic lactic acid bacteria from donkey,s milk

Background : The utilization of donkey&rsquo;s milk is increasing with regard to nutritional values and non-allergenic proteins. Donkey&rsquo;s milk microbiota, especially lactic acid bacteria (LAB) has not yet been fully identified. This study was conducted to identify and isolate LAB with probiotic properties. Materials and Methods: Specific culture-dependent techniques and standard microbiology methods were used to identify LAB. 250 isolates were randomly picked from different donkey&rsquo;s milk samples different farms in Alashtar, north of Lorestan province, Iran. Gram-staining, catalase reaction, motility test, growth at different temperatures, pH 9.6 and in presence of 6.5% NaCl as well as the ability to ferment different sugars, sodium hippurate hydrolysis and light microscope analysis were investigated. Results: Out of 250 isolates, 20 isolates were gram-positive, catalase-negative and cocci form. Enterococcus and Streptoccus genera were dominant LAB in donkey&rsquo;s milk. Entreococcus faecalis (55%) and Streptococcus devriesei (45%) were among the predominant species. The results of this experiment, for the first time, reported the occurrence of Streptococcus devriesei in donkey&rsquo;s milk in Iran. Conclusion: Despite high lysozyme concentration, donkey&rsquo;s milk rich in probiotic LAB can be used to feed infants with allergy to cow&rsquo;s milk proteins. Both Entreococcus and Streptococcus bacteria produce different types of bacteriocins which are effective against a wide range of different type of infections. Occurrence of Streptococcus devriesei in donkey,s milk may provide new research field in Iran