Hubungan antara Homosistein dan Nitrit Oksid pada Hipertensi Esensial di Jawa Tengah, Indonesia

Background: The essential hypertension has been related to endothelium-dependent vasodilatation disorders that result of decreasing of nitric oxide (NO) bioavailability. Decreasing of NO can be caused by increase of homocysteine level.Objective: This study is aimed at finding out the effect of homocysteine level on nitric oxide level in essential hypertension, and relationship between NO and blood pressure and hypertension risk in Central Java.Method: This study was case-control nested, in “Surveillance of Non-Communicable Diseases” in Purworejo, Central Java. The subjects were men with essential hypertension and 20-60 years old that had normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They didn\u27t take antihypertension drugs. The methods of Pfeiffer, et al. was used to identify total plasma homocysteine level. NO level was determined by Nitric Oxide Colorimetric Assay Kit (BioVision Cat.K262200).Result: The homocysteine level of the hypertension is higher than that of the non- hypertension (16.15 + 12.69 vs 12.73 + 5.73; P=0.035), whereas the nitric oxide level of the hypertension is lower than that of the non-hypertension (8.22 + 1.88 vs 9.49 + 3.05; P=0.003). Homcysteine level was significantly correlated with nitric oxide level (P=0,016). Negative correlation between nitric oxide level and systolic and diastolic pressures was significant (P=0,005; P=0,045), respectively in subjects of 40-60 years old but not significant (P=0,106; P=0,269), respectively in those younger (20-39 years old). The relationship between nitric oxide and hypertension risk was significant (P=0,005) in subjects of 40-60 years old but not significant (P=0,062) in those younger (20-39years old).Conclusion: The high homocysteine level is a risk factor of hypertension, that reduces the NO bioavailability. The reducing of NO bioavailability was related to increase of blood pressure and hypertension risk, especially it was in the old men

Hubungan antara Homosistein dan Nitrit Oksid pada Hipertensi Esensial di Jawa Tengah, Indonesia

Background: The essential hypertension has been related to endothelium-dependent vasodilatation disorders that result of decreasing of nitric oxide (NO) bioavailability. Decreasing of NO can be caused by increase of homocysteine level.Objective: This study is aimed at finding out the effect of homocysteine level on nitric oxide level in essential hypertension, and relationship between NO and blood pressure and hypertension risk in Central Java.Method: This study was case-control nested, in “Surveillance of Non-Communicable Diseases” in Purworejo, Central Java. The subjects were men with essential hypertension and 20-60 years old that had normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They didn’t take antihypertension drugs. The methods of Pfeiffer, et al. was used to identify total plasma homocysteine level. NO level was determined by Nitric Oxide Colorimetric Assay Kit (BioVision Cat.K262200).Result: The homocysteine level of the hypertension is higher than that of the non- hypertension (16.15 + 12.69 vs 12.73  + 5.73; P=0.035), whereas the nitric oxide level of the hypertension is lower than that of the non-hypertension (8.22 + 1.88 vs 9.49 + 3.05; P=0.003). Homcysteine level was significantly correlated with nitric oxide level (P=0,016). Negative correlation between nitric oxide level and systolic and diastolic pressures was significant (P=0,005; P=0,045), respectively in subjects of 40-60 years old but not significant (P=0,106; P=0,269), respectively in those younger (20-39 years old). The relationship between nitric oxide and hypertension risk was significant (P=0,005) in subjects of 40-60 years old but not significant (P=0,062) in those younger (20-39years old).Conclusion: The high homocysteine level is a risk factor of  hypertension, that reduces the NO bioavailability. The reducing of NO bioavailability was related to increase of blood pressure and hypertension risk, especially it was in the old men.Keywords: homocysteine, nitric oxide, hypertension, Central Java, Indonesi

Effect of C677T Mutation in Methiylenetetrahydrofolate Reductase Gene and Blood Folate on Hypertension Risk

Background: The prevalence of hypertension risk factor varies among race or ethnic groups. The varied prevalence is associated with culture or genetic differences or interaction between genetic and environment or nutrition factorsObjective: The aim of this study was to identify relationship between polymorphism of C677T in MTHFR gene and folate status towards the risk of hypertension among Javanese population.Methods: This study was case-control nested, in "Surveillance of Non-Communicable Diseases" in Purworejo, Central Java. The subjects were men with essential hypertension and 20-60 years old that had normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They didn't take antihypertension drugs. The analysis of C677T polymorphism in MTHFR gene was done according to Frosst et al. (1995) method. The blood folate level was determined by Delfia-Folate Kit (PerkinElmer). The methods of Pfeiffer et al. (1999) was used to identify plasma total homocysteine level.Results: Odds ratio for subjects with CT genotype was 1.36 ICI: 95%:0.50;3.70); folate deficiency was 1.40 ICI: 95%:0.57;3.451; and both CT genotype and folate deficiency was 2.30 (CI: 95%:0.45;15.001. Homocysteine level of the cases with both folate deficiency and CT genotype was 45.28 .:!:. 30.39; and only folate deficiency was 17.94.:!:. 12.77; whereas in the control with both folate deficiency and CT genotype was 21.44.:!:. 10.71 and only folate deficiency was 11.79.:!:. 5.28.Conclusions: The effect of folate deficiency to homocysteine level and hypertension risk was slighly stronger than the genetic effect of C677T in MTHFR gene. In subjects with normal folate level, the CT genotype did not influence homocysteine level.Kata kunci:polymorphism, methylenetetrahydrofolate reductase, folate, hypertensio

Effect of C677T Mutation in Methiylenetetrahydrofolate Reductase Gene and Blood Folate on Hypertension Risk

ABSTRACT Sunarti, Ahmad Husain Asdie, Mohammad Hakimi, Abdul Salam M Sofro - Effect of C677T mutation In methylenetetrahydrofolate reductase gene and blood folate on hypertension risk Background: The prevalence of hypertension risk factor varies among race or ethnic groups. The varied prevalence is associated with culture or genetic differences or interaction between genetic and environment or nutrition factors Objective: The aim of this study was to identify relationship between polymorphism of C677T in MTHFR gene and folate status towards the risk of hypertension among Javanese population. Methods: This study was case-control nested, in "Surveillance of Non-Communicable Diseases" in Purworejo, Central Java. The subjects were men with essential hypertension and 20-60 years old that had normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They didn\u27t take antihypertension drugs. The analysis of C677T polymorphism in MTHFR gene was done according to Frosst et al. (1995) method. The blood folate level was determined by Delfia-Folate Kit (PerkinElmer). The methods of Pfeiffer et al. (1999) was used to identify plasma total homocysteine level. Results: Odds ratio for subjects with CT genotype was 1.36 ICI: 95%:0.503.70)folate deficiency was 1.40 ICI: 95%:0.573.451and both CT genotype and folate deficiency was 2.30 (CI: 95%:0.4515.001. Homocysteine level of the cases with both folate deficiency and CT genotype was 45.28 .:!:. 30.39and only folate deficiency was 17.94.:!:. 12.77whereas in the control with both folate deficiency and CT genotype was 21.44.:!:. 10.71 and only folate deficiency was 11.79.:!:. 5.28. Conclusions: The effect of folate deficiency to homocysteine level and hypertension risk was slighly stronger than the genetic effect of C677T in MTHFR gene. In subjects with normal folate level, the CT genotype did not influence homocysteine level. Kata kunci: polymorphism, methylenetetrahydrofolate reductase, folate, hypertensio