Pengujian Aktivitas Antiokidan Ekstrak Terpurifikasi Daun Teh Hijau dan Jati Belanda

Teh hijau memiliki nama spesies Camellia sinensis (L.) Kuntze, family Theaceae dan Jati belanda (Guazuma ulmifolia Lam.) termasuk kedalam family sterculiaceae. Ekstrakse dengan metode maserasi termodifikasi microwave dengan pelarut air. Purifikasi ekstrak dengan menggunakan pelarut dengan tingkat kepolaran yang berbeda-beda dimulai dengan petroleum eter, kloroform, etil asetat dan metanol. Pengujian aktivitas antioksidan dengan metode peredaman radikal DPPH. Hasil penelitian dipeoleh rendamen ekstrak terpurifikasi ekstrak teh hijau 13,33 % dan jati belanda 46,66 %.Pengujian antioksidan menunjukkan nilai IC50 jati belanda yaitu 10,13 ppm, lebih besar dibandingkan teh hijau yaitu 31,13 %

Uji Toksisitas Ekstrak Daun Pletekan (Ruellia Tuberosa L.) dengan Menggunakan Metode Brine Shrimp Lethality Test (Bslt)

Minnie root (Ruellia tuberosa L.) belongs to Acanthaceae family. In traditional medicine has been used as diuretic, antidiabetic, antipyretic and antihypertensive and it also recently been incorporated as a component in a herbal tea in Taiwan. This research is aim to determine LC50 value of Minnie root leaves extract (Ruellia tuberosa L.) to Artemia salina Leach shrimp larvae. Dryed samples extracted by graduate maceration with n-hexan, ethyl acetate and ethanol respectively. Rendamen n-hexane extract obtained as much as 1.158%, ethyl acetate extract as much as 2.017% and ethanol extract as much as 3.669 %. Based on qualitative screening used TLC method, ethyl acetate extracts and ethanol extracts of Minnie root (Ruellia tuberosa L.) leaves contain alkaloid, saponin, flavonoid and phenolic compounds. Toxicity assay Artemia salina Leach shrimp larvae have been conducted on leaves extracts of Minnie root (Ruellia tuberosa L.). Toxicity assay on sample extract of Minnie root (Ruellia tuberosa L.) leaves determine by counting the number died of larvae after 24 hours treatment. LC50 on sample calculated by probit analysis method. The toxicity assay showed LC50 of ethyl acetate extracts and ethanol extracts are toxic with LC50 266.07 ± 0.93 µg/mL and 142.160 ± 1.30 µg/mL

Studi Komparasi Kadar Flavonoid Total pada Bunga Rosella Merah (Hibiscus Sabdariffa L.) Asal Kabupaten Luwu Utara Provinsi Sulawesi Selatan dan Kabupaten Kediri Provinsi Jawa Timur

Red Roselle (Hibiscus sabdariffa L.) is a medicinal plant used by the public as drugs. This plant is efficacious because one contains chemical constituents are flavonoids. Comparative Study of total Flavonoid content in red Roselle (Hibiscus sabdariffa L.) from North Luwu district of South Sulawesi province and the province of East Java Kediri district has done. The aim of study to determine of total flavonoid content in Kediri regency and district of North Luwu using a spectrophotometer. Extraction is done by maceration method using 96% ethanol. Phytochemical screening using the color reaction identification and determine content of Colorimetric method using UV-Vis spectrophotometry at the maximum wavelength of 417 nm. Meanwhile the yield extract red Rosellafrom Kediri (sample I) 12.703% and red Rosella from north Luwu (sample II) 10.44%. Test phytochemical to sample I and sample II positive containing Flavonoids. Test total Flavonoid content to sample I is 0.02816% and sample II is 0.2075%

Uji Aktivitas Larvasida Ekstrak Daun Jarak Kepyar (Ricinus Communis L.) Terhadap Larva Nyamuk Aedes Aegypti

Castor Plant (Ricinus communis L.) belongs to Euphorbiaceae family. In medicine has been used as antioxidant, antihistaminic, antifertility, antimicrobial, antidiabetic, antiinflammatory, antipyretic, and it has activities to larvacidal, molluscicidal and insecticidal. The aim of this study was to determine the level of mosquito larva (Aedes aegypti) mortality after giving of ethanolic extract showed with LC50. Extract obtained by maceration method, rendamen ethanolic extract obtained as much as 6.073%. This research uses 110 instar, III larvae of mosquito (Aedes aegypti), divided into test solution are made with 3 concentrations (10 µg/mL, 100 µg/mL, 1000 µg/mL), positive control using Abate and negative control using water. Observations were made after 24 hours of treatment. Toxicity test results shows ethanolic extract is an extract that is active as Larvacidal showed LC50 as much as 138,995 ± 1,5 µg/mL < 1000 µg/mL. Based on qualitative testing of the chemical component using TLC method known ethanolic extract of Castor Plant (Ricinus communis L.) leaves contain alcaloids, saponins and flavonoids

Standarisasi Ekstrak Air Daun Jati Belanda dan Teh Hijau

Teh hijau memiliki nama spesies Camellia sinensis (L.) Kuntze, family Theaceae dan Jati belanda (Guazuma ulmifolia Lam.) termasuk kedalam family sterculiaceae Tujuan Penelitian ini adalah melakukan standarisasi ekstrak air daun jati belanda dan ekstrak air the hijau. Ekstrak distandardisasi dengan beberapa dua parameter yaitu parameter spesifik dan parameter non spesifik. Kadar sari larut air pada jati belanda 12,88 % dan teh hijau 40,88, sedangkan kadar sari larut etanol pada jati belanda 4, 23 % dan pada teh hijau 4,23 %. Hasil pengujian kandungan kimia menunjukkan pada ekstrak jati belanda mengandung saponin dan flavonoid sedangkan pada teh hijau mengandung tanin dan flavonoid. Kadar air ekstrak daun jati belanda 0,95 % dan teh hijau 2,79%. Hasil kadar abu total jati belanda sebesar 37,61% dan teh hijau 36,84%. Kadar abu tidak larut asam yaitu pada jati belanda sebesar 3,54% dan teh hijau 3,77%. Hasil dari penetapan susut pengeringan pada ekstrak jati belanda yaitu 0,46 % dan teh hijau 0,46 %. Ekstrak jati belanda maupun teh hijau berdasarkan pengujian standarisasi meliputi parameter spesifik dan non-spesifik memenuhi standarisasi mutu bahan baku