Cutting Edge: Differential Regulation of Chemoattractant Receptor-Induced Degranulation and Chemokine Production by Receptor Phosphorylation

Phosphorylation of G protein-coupled receptors and the subsequent recruitment of β-arrestin play an important role in desensitization of receptor-mediated responses, including degranulation in leukocytes. In this study, we report that receptor phosphorylation also provides a stimulatory signal for CCR ligand 2 (CCL2) production. C3a stimulated degranulation in a basophilic leukemia RBL-2H3 cell expressing wildtype C3aR or a phosphorylation-deficient mutant (ΔST-C3aR). In contrast, C3a caused CCL2 production only in C3aR but not eΔST-C3aR cells. Furthermore, overexpression of G protein-coupled receptor kinase 2 resulted in enhancement of both ligand-induced receptor phosphorylation and CCL2 production but inhibition of degranulation. Agonist activation of C3aR, but not ΔST-C3aR, led to the translocation of green fluorescent protein tagged β-arrestin 2 from the cytoplasm to the plasma membrane. These data demonstrate that receptor phosphorylation, which provides a turn off signal for degranulation, is essential for CCL2 production

An Impregnable Lightweight Device Discovery (ILDD) Model for the Pervasive Computing Environment of Enterprise Applications

The worldwide use of handheld devices (personal digital assistants, cell phones, etc.) with wireless connectivity will reach 2.6 billion units this year and 4 billion by 2010. More specifically, these handheld devices have become an integral part of industrial applications. These devices form pervasive ad hoc wireless networks that aide in industry applications. However, pervasive computing is susceptible and vulnerable to malicious active and passive snoopers. This is due to the unavoidable interdevice dependency, as well as a common shared medium, very transitory connectivity, and the absence of a fixed trust infrastructure. In order to ensure security and privacy in the pervasive environment, we need a mechanism to maintain a list of valid devices that will help to prevent malicious devices from participating in any task. In this paper, we will show the feasibility of using a modified human- computer authentication protocol in order to prevent the malicious attacks of ad hoc networks in industrial applications. We will also present two separate models for both large and small networks, as well as several possible attack scenarios for each network

AWARENESS AND USAGE OF SWAYAM COURSES AMONG LIBRARY AND INFORMATION SCIENCE STUDENTS: A SURVEY

The Government of India initiated SWAYAM (Study Webs of Active-learning for Young Aspiring Minds), where the objective of the programme is to take the best teaching and learning resources to all with no costs. To find out the awareness and usage of SWAYAM courses among Library and Information science students, survey method of research used. To collect data from selective Universities students a structured questionnaire was prepared. The results show that most of the responses received from Annamalai University, majority respondents are aware of SWAYAM course. Respondents are aware of these courses through their teachers. 74.73% of respondents replied that their University providing orientation programme on SWAYAM. Half of the respondents prefer Word file format for submitting their assignment. More than 82% of respondents are spending 1-3 hours for SWAYAM course. Half of the respondents are agreed SWAYAM courses help to gain new knowledge and supports life-long learning. Further, the result reveals that the numbers of students continue their enrolled courses and only less number were discontinued due to some reason

OPEN ACCESS RESOURCES IN SCIENCE AND TECHNOLOGY: AN OVERVIEW.

Open access resources includes open access archives/repositories, open access journals, open access books, open access search engines and open educational resources etc., the objective of the study is to find open access resources available in Science and Technology. To achieve this objective the investigator visited several Science & Technology University library websites and research organisations websites to locate the resources. In the findings investigator discussed the several resources related to Science and Technology like open DOAR, PubMed Central, arXiv, BioMed Central, DOAJ, Bentham Open, PLOS Journals, Hindawi journals, Springer Open, Wiley open access journals, Science Direct open access journals, NISCAIR Journals, Indian Academy of Sciences journals, Indian Journals.com, E-Books Directory, DOAB, Shodhganga, NPTEL, Swayam etc.

Efficacy of budesonide nasal irrigation in comparison to normal saline nasal irrigation in post-operative management of endoscopic sinus surgery: A double blinded randomized control study

BACKGROUND : Nasal irrigation with normal saline and topical steroid spray form the mainstay of treatment in post endoscopic sinus surgery patients. However nasal sprays may not deliver optimum dosage of drug to the paranasal sinus mucosae. Budesonide nasal irrigation solves this problem by delivering drugs in a high volume high pressure system. TRIAL DESIGN: Double blinded randomized control trial. METHODS : 88 patients were recruited and randomized into 2 groups of 44 each. One arm received normal saline nasal irrigation. The other arm received budesonide nasal irrigation (0.5mg in 200ml) twice daily. Both groups received steroid nasal spray as a part of standard care. Patients were followed up at 2 weeks post-op and 3 months post-op and on each visit SNOT 22 and Lund Kennedy Endoscopic scores were assessed. RESULTS: The mean scores in the first visit was SNOT22 : 30.98+2.93 vs 26.64+2.32 (p<0.0001) and LKES : 4.53+0.76 vs 4.08+0.78 (p=0.0034) in the saline and budesonide groups respectively. The mean scores in the second visit was SNOT 22 : 17.63+4.92 vs 11.47+4.04 (p<0.0001) and LKES : 2.61+1.03 vs 1.50+1.01 (p<0.0001) in the saline and budesonide groups respectively. Thus budesonide arm had statistically significantly better scores when compared to normal saline group. CONCLUSION: Budesonide nasal irrigation with positive pressure high volume device was found to have better efficacy when compared to normal saline irrigation. Budesonide nasal irrigation may be used in the post-operative management of endoscopic sinus surgery patients

Identification of suitable media based on hydroponic culture for production Zucchini squash

An experiment was conducted to identify the hydroponic culture based suitable media for the production of Zucchini Squash in the Biochemistry Laboratory, Patuakhali Science and Technology University, Dumki, Patuakhali, Bangladesh during 2014. Zucchini plant (Cucurbita pepo L.) were grown in closed soilless systems to determine the effect of four different hydroponics media on plant growth, yield and nutrient contents (fruit moisture content, ascorbic acid content on fruit, fruit protein content, protein content in leaves). Three types of substrates (coconut husk, jute, cotton) along with Hoagland solution were used in this experiment. Result revealed that media using Jute fiber showed significant effect on plant growth and nutritional values than the other media (media of cotton with Hoagland solution, coconut husk with Hoagland solution and only Hoagland solution). The plant grown using jute media showed the highest plant height (60.33 cm), number of leaves (17.33), yield (1.5 kg plant-1), fruit moisture content (97.33%), Ascorbic acid content in fruit (28.73 mg 100g-1), protein percentage in fruit (1.406%) and percentage (1.326%) in leaves than the other media. Therefore, with the controlled nutrient supply, less expense, less labor, no use of pesticides or fertilizer with controlled environment the use of jute fiber as a substrate with Hoagland solution can be an effective one.Int. J. Agril. Res. Innov. &amp; Tech. 6 (2): 1-4, December, 201

Review of 14 drowning publications based on the Utstein style for drowning

Abstract Background The Utstein style for drowning (USFD) was published in 2003 with the aim of improving drowning research. To support a revision of the USFD, the current study aimed to generate an inventory of the use of the USFD parameters and compare the findings of the publications that have used the USFD. Methods A search in Pubmed, Embase, the Cochrane Library, Web of Science and Scopus was performed to identify studies that used the USFD and were published between 01-10-2003 and 22-03-2015. We also searched in Pubmed, Embase, the Cochrane Library, Web of Science, and Scopus for all publications that cited the two publications containing the original ILCOR advisory statement introducing and recommending the USFD. In total we identified 14 publications by groups that explicitly used elements of the USFD for collecting and reporting their data. Results Of the 22 core and 19 supplemental USFD parameters, 6–19 core (27–86%) and 1–12 (5–63%) supplemental parameters were used; two parameters (5%) have not been used in any publication. Associations with outcome were reported for nine core (41%) and five supplemental (26%) USFD parameters. The USFD publications also identified non-USFD parameters related to outcome: initial cardiac rhythm, time points and intervals during resuscitation, intubation at the drowning scene, first hospital core temperature, serum glucose and potassium, the use of inotropic/vasoactive agents and the Paediatric Index of Mortality 2-score. Conclusions Fourteen USFD based drowning publications have been identified. These publications provide valuable information about the process and quality of drowning resuscitation and confirm that the USFD is helpful for a structured comparison of the outcome of drowning resuscitation

Awareness And Usage of Swayam Courses Among Library And Information Science Students: A Survey

The Government of India initiated SWAYAM (Study Webs of Active-learning for Young Aspiring Minds), where the objective of the programme is to take the best teaching and learning resources to all with no costs. To find out the awareness and usage of SWAYAM courses among Library and Information science students, survey method of research used. To collect data from selective Universities students a structured questionnaire was prepared. The results show that most of the responses received from Annamalai University, majority respondents are aware of SWAYAM course. Respondents are aware of these courses through their teachers. 74.73% of respondents replied that their University providing orientation programme on SWAYAM. Half of the respondents prefer Word file format for submitting their assignment. More than 82% of respondents are spending 1-3 hours for SWAYAM course. Half of the respondents are agreed SWAYAM courses help to gain new knowledge and supports life-long learning. Further, the result reveals that the numbers of students continue their enrolled courses and only less number were discontinued due to some reason