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AQP2 mRNA levels in the renal outer medulla.

<p>AQP2 mRNA levels are expressed as relative values from control untreated rats. The following formula was applied: ΔΔCT = (<i>CTAQP</i>2−<i>CTGAPDH</i>) <i>experimental</i>−(<i>CT AQP</i>2−<i>CT GAPDH</i>)<i>control untreated rats</i>. Two-way ANOVA showed a statistically significant (p<0.001) interaction between the effects of Diabetes and L-Arg treatment on AQP2 mRNA expression. Bonferroni’s post- tests: *p<0.05 vs. control untreated rats; ***p<0.001 vs. control untreated rats; &&&p<0.001 vs. diabetic untreated rats. Data are mean ± SEM (n = 6).</p

Summary of some general and renal parameters in control and diabetic rats.

<p>C: control rats; D: diabetic untreated rats; D+A: diabetic rats treated with L-Arg; C+A: control rats treated with L-Arg. Results are expressed as the mean ± SEM (n = 6).</p><p>Two-way ANOVA showed a statistically significant interaction (p<0.05) between the effects of Diabetes and L-Arg treatment on NOx urinary excretion. There was no interaction between the effects of Diabetes and L-Arg treatment on the other parameters. Diabetes had a significant overall effect on Final body weight (p<0.001), Kidney weight/body weight (p<0.001), Blood glucose (p<0.001), Urinary volume (p<0.05), Osmolar excretion rate (p<0.01), Urinary glucose (p<0.001) and Urinary ketones (p<0.001). L-Arg had no significant effect on any of the parameters tested. *p<0.05 vs. C; **p<0.01 vs. C; ***p<0.001 vs. C; ^#p<0.05 vs. C+A; ^##p<0.01 vs. C+A ^###p<0.001 vs. C+A; ^&&&p<0.001 vs. D.</p

NOS activity measured as pmol [¹⁴C] L-citrulline/g tissue/min.

<p>Two-way ANOVA showed no statistically significant interaction between the effects of Diabetes and L-Arg treatment on NOS activity. The effects of Diabetes and L-Arg were considered extremely significant (p<0.001). ***p<0.001 vs. control untreated rats; ^###p<0.001 vs. control rats treated with L-Arg; ^&&&p<0.001 vs. diabetic untreated rats. Data are mean ± SEM (n = 8).</p