Optimization of production, biochemical characterization and In Vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus Amyloliquefaciens

The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.We express our thanks to Coordination for the Improvement of Higher Level Education Personnel (CAPES) - Doctoral Sandwich Program (PDSE) Nº 0259/ 12-8 and National Council for Scientific and Technological Development (CNPq) - Nº 202026/2011-6 for the financial support

Integrated optimal design of permanent magnet synchronous generator for smart wind turbine using genetic algorithm

In recent years, the investment in the wind energy sector has increased in the context of producing green electricity and saving the environment. The installation of small wind turbines (SWTs) represents an actual strategy for meeting energy needs for off-grid systems and certain specialized applications. SWTs are more expensive per kilowatt installed as compared to large-scale wind turbines. Therefore, the main objective of this study is to produce an economical technology for the wind power market offering low-cost SWTs. The idea consists of considering a simple structure of the wind turbine using direct-drive permanent magnet synchronous generator (DDPMSG). DDPMSGs are the most useful machines in the wind energy field thanks to several advantages, such as elimination of noise and maintenance cost due to suppression of the gearbox and absence of the rotor circuit excitation barriers by the presence of the permanent magnets (PMs). Their major downside is the high cost of active materials, especially the PMs. Thus, the improvement of the generator design is treated as being the main component of the considered chain to assure active materials’ mass and cost reduction. The methodology studied aims to explain the approach of the design integrated by optimization of the considered system. It is based on the elaboration of analytical models to find a feasible structure for the system, taking into account the multi-disciplinary analysis. The relevance of these models is validated by the finite element method using 2D MATLAB-FEMM simulation. The models are integrated to elaborate the optimization problem based on a genetic algorithm to improve the cost of the proposed generator by minimizing the mass of its active constructive materials. As an outcome, an optimal solution is offered for the wind generators market, providing a 16% cost reduction

Beekeepers perception of risks affecting colony loss: A pilot survey

Understanding amateur beekeepers’ perception of risks affecting bee health and mortality is essential to analyse the reasons for adopting or rejecting good management practices. A perception survey on how beekeepers perceive and manage factors related to climate change, Varroa infestation, management practices, and pesticide exposure was designed and launched online. This unpreceded sociological survey involved 355 beekeepers spread all over Belgium. A two-sample t test with unequal variances comparing beekeepers with colony loss rates below or exceeding the acceptable level, that is <10% and ≥10%, indicates that beekeepers (N = 213) with colony loss rates <10% generally have greater average levels of perceived risks and the benefits of action that lead to increased motivation to act in better ways. The results of this survey highlight the importance of looking beyond socio-economic determinants in any risk mitigation strategy associated with bee mortality when dealing with amateur beekeepers. © 2021 Wiley-VCH Gmb

Screening for renal impairment in viral hepatopathy B: it is time to begin

Aim: The aim of this study was to assess the value of routine screening for renal damage in cases of B viral infection, by looking for proteinuria and elevated creatinemia. Materials & methods: We investigate the frequency and associated factors of renal impairment in patients with viral B hepatopathy. Results: Pathological albuminuria was confirmed in 44.73%. The chronic kidney disease with slightly decreased filtration rate was found in 21.05%. In multivariate analysis, only BMI was an independent factor for pathological albuminuria (p = 0.013) and only age was an independent predictor of chronic kidney disease (p = 0.056). Conclusion: Urine dipstick and creatinemia are useful for routine screening in viral B hepatopathy especially in the elderly and overweight