Comparison of Vitreous Protein Profiles of Rabbits subjected to Acute Carbon Monoxide poisoning and normal animal after death

Acute carbon monoxide (CO) poisoning is caused by the inhalation of large quantity of CO within short time leading to morbidity and mortality. In this study, a total of eighteen (18) rabbits were divided into three (3) groups of six (6) animals each. The first group first group was poisoned with CO and labelled carbon monoxide death (CD). The second group of animals was killed prior to exposure to CO and labelled disguised death (DD), while the third was the control group (CG). The vitreous humour samples were collected from all the animals using standard methodologies. The vitreous humour total protein and albumin were estimated while its globulin content and albumin/globulin ratio were derived mathematically. Pearson correlation analyses were used to establish relationships between vitreous protein profile components and the acute graded carbon monoxide concentrations. Results indicated a significant increase (P<0.05) in vitreous total protein and globulin of the studied groups. This observation was more pronounced in the CD group of animals. Therefore the marked elevation of vitreous total protein and globulin may serve as a useful tool in the confirmation of death due to carbon monoxide poisoning

Relationships between Serum Globulins, Albumin/Globulin Ratio and C-Reactive Proteins In Pregnancy Trimesters

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Vitreous Humour Lipid Peroxidation as an Emerging Concept of Acute Carbon Monoxide Poisoning

Carbon monoxide (CO) is a euphoric poisonous gas with the predisposition of causing death depending on its concentration. This study was designed to assess the effects of acute CO poisoning on oxidative stress indicators and other ancillary biochemical parameters. Eighteen (18) rabbits divided into three groups of six each constituted the sample size. The groups comprised of controls (CG), disguised death (DD) and CO death (CD). The CO utilized for the study was a product of portable generating set (SUMEC). Vitreous humor was extracted from the eyes of rabbits and assayed for lipid peroxidation parameters such as catalase (CAT), superoxide dismutase (SOD), malonaldehyde (MDA), glutathione reducatase (GT), lipid profiles, uric acids and glucose using standard methods. The result showed that vitreous SOD was significantly lower (P < 0.05) in both the DD and CD, but more markedly lower in the CD. On the contrarily, vitreous CAT was significantly higher (P < 0.05) in the DDs. Also, vitreous MDA exhibited significant increase (P < 0.05) in both the DDs and CDs. Vitreous GT showed no significant difference (P > 0.05). The vitreous lipid profiles result showed a pan markedly significant decrease (P < 0.05) in the CDs as compared to the DDs. Vitreous uric acid exhibited a significant decrease only in the CDs, whereas vitreous glucose decreased both in the DD and CD, but more pronounced in the DD. Therefore, the findings showed that lipid peroxidation is a phenomenon associated with acute carbon monoxide poisoning