Remediation of zinc contaminated soils from a farm settlement in Nigeria

This work is on remediation of zinc concentration (330.0 mg/kg) in soils from farm settlement at Agbabu community in Ondo State of Nigeria to below maximum allowable 300 mg/kg specified for safe agriculture by standards to ensure that farm products from this farm settlement close to area of mining are safe for human beings. Three indigenous organisms: Bacillus subtilis (B. subtilis), Escherichia coli (E. coli) and Proteus mirabilis (P. mirabilis) were engaged for the remediation study. The organisms were isolated and cultured. Optimum weights of the distinct organisms were inoculated in 4g soils each conditioned with optimum values of pH, temperature, stirring frequency and nutrient in thirty-six 50 ml beakers; and experimented for residual zinc ion at times 5, 10, 15, 20, 25, 30 and 35 days in triplicate with Atomic Absorption Spectrophotometer. Each organism maintained its performance position from day 5 to day 35. Bacillus subtilis took the lead, seconded by P. mirabilis while E. coli lagged. Removal to safe concentration first occurred at 10 days for B. subtilis, 15 days for P. mirabilis and 20 days for E. coli with respective 292.09 mg/kg, 294.37 mg/kg, and 290. 71 mg/kg residual concentrations. The respective residual concentrations and efficiencies at 35 days were 247.33 mg/kg and 25.06 %; 253.47 mg/kg and 23.20 %; and 267.11 mg/kg and 19.07 %. Two-ways ANOVA at (P < 0.05) showed that a combination of 2 or 3 of the organisms would result in lower residual concentration; and relevant performances at shorter times. Keywords: Zinc, contaminated soils, farm settlement, bioremediatio

Serum magnesium and calcium levels in relation to ischemic stroke : Mendelian randomization study

ObjectiveTo determine whether serum magnesium and calcium concentrations are causally associated with ischemic stroke or any of its subtypes using the mendelian randomization approach.MethodsAnalyses were conducted using summary statistics data for 13 single-nucleotide polymorphisms robustly associated with serum magnesium (n = 6) or serum calcium (n = 7) concentrations. The corresponding data for ischemic stroke were obtained from the MEGASTROKE consortium (34,217 cases and 404,630 noncases).ResultsIn standard mendelian randomization analysis, the odds ratios for each 0.1 mmol/L (about 1 SD) increase in genetically predicted serum magnesium concentrations were 0.78 (95% confidence interval [CI] 0.69-0.89; p = 1.3 7 10-4) for all ischemic stroke, 0.63 (95% CI 0.50-0.80; p = 1.6 7 10-4) for cardioembolic stroke, and 0.60 (95% CI 0.44-0.82; p = 0.001) for large artery stroke; there was no association with small vessel stroke (odds ratio 0.90, 95% CI 0.67-1.20; p = 0.46). Only the association with cardioembolic stroke was robust in sensitivity analyses. There was no association of genetically predicted serum calcium concentrations with all ischemic stroke (per 0.5 mg/dL [about 1 SD] increase in serum calcium: odds ratio 1.03, 95% CI 0.88-1.21) or with any subtype.ConclusionsThis study found that genetically higher serum magnesium concentrations are associated with a reduced risk of cardioembolic stroke but found no significant association of genetically higher serum calcium concentrations with any ischemic stroke subtype