Mediterranean diet adherence and synergy with acute myocardial infarction and its determinants. a multicenter case-control study in Italy.

Cardiovascular diseases are the leading causes of mortality and morbidity in Western countries. The possible synergistic effect of poor adherence to a Mediterranean diet (MD) and other risk factors for acute myocardial infarction (AMI) such as hypertension, cholesterol, ever smoker, BMI> 25, diabetes, has not been deeply studied. Design Case-control study. Methods Patients with first AMI and controls from four tertiary referral Italian centers were screened for enrolment. Dietary information was collected through a questionnaire and a MD adherence score was calculated. Physical activity and smoking habits were also registered. The Synergy Index was calculated according to Rothman. Results 127 cases and 173 controls were enrolled. The analysis was conducted using a dichotomous variable for the MD score with values 7 representing good adherence. Multivariate analysis showed the following variables associated to AMI: ever smoker (OR = 2.08), diabetes (OR = 1.42), hypertension (OR = 2.08), hypercholesterolemia (OR = 2.47), BMI> 25 (OR = 1.99), while a protective effect emerged both in subjects scoring > 7 on the MD score(OR = 0.55) and in subjects resident of Southern Italy (OR = 0.38). A synergistic effect does exist between poor adherence to the MD and the following risk factors: hypertension, hypercholesterolemia, BMI >25, diabetes and being a resident in central and northern Italy. Conclusion Synergy between heart disease risk factors and MD underlines the need to enlarge the list of known modifiable cardiovascular risk factors to include and promote adherence to Mediterranean dietary habits

Rapid and non-destructive method to assess in the vineyardgrape berry anthocyanins under different seasonal andwater conditions

Abstract Background and Aims: Monitoring of anthocyanins (Anth) in winegrape (Vitis vinifera L.) is fundamental for the production of top-quality red wines. This work was aimed at testing a new fluorescence-based sensor for Anth detection in the vineyard. Significance of the Study: The present study showed a new important innovative technology for viticulture. The sensor evaluation of the large spatial and temporal heterogeneity in Anth accumulation can be useful as support parameter in the harvest date decision or for vineyard zoning of phenolic maturity. Methods and Results: Anth in grape (cv. Aleatico) bunches attached to the vine were monitored non-destructively in the field using a fluorescence-based sensor during the 2008 and 2009 seasons and under different water regimes. The ANTHRG index = log(far-red-fluorescenceR/far-red-fluorescenceG), with fluorescence signals excited with red (R) and green (G) light, was inversely correlated through an exponential function (r2 = 0.875) to the Anth concentration derived from the HPLC analysis of berry skin extracts. ANTHRG was effective in detecting the earlier ripening process in 2009 with respect to 2008 and differences in the Anth accumulation between seasons and in relation to different water regimes.Water deficit imposed in 2009 enhanced Anth concentration in berries because of a reduction in berry size but also an increase of Anth biosynthesis. This effect was observed by both destructive and ANTHRG non-destructive measurements. Conclusions: Our results show that the employed fluorescence sensor represents a reliable, rapid and non-invasive tool for monitoring and determining Anth accumulation in situ

Short-Term Pre-Harvest UV-B Supplement Enhances the Polyphenol Content and Antioxidant Capacity of Ocimum basilicum Leaves during Storage

Ocimum basilicum (basil) leaves are rich in polyphenols, conferring them a high antioxidant activity. The application of UV-B can be used to maintain the post-harvest nutraceutical quality of basil leaves. We aimed to investigate the effects of pre-harvest UV-B application on polyphenolic and pigment contents, antioxidant capacity, and the visual quality of basil stored leaves. We also evaluated the applicability of the non-invasive Dualex® for monitoring the accumulation of leaf epidermal phenolics (Flav Index). After exposing plants to white light (control) and to supplemental UV-B radiation for 4 d, the leaves were harvested and stored for 7d (TS7). The UV-B leaves showed both a higher phenolic content and antioxidant capacity than the controls at TS7. In addition, the correlations between the Flav Index and phenolic content demonstrated that Dualex® can reliably assess the content of epidermal phenolics, thus confirming its promising utilization as a non-destructive method for monitoring the phytochemical quality of O. basilicum leaves. In conclusion, a pre-harvesting UV-B application may be a tool for enhancing the content of polyphenols and the antioxidant potential of basil stored leaves without detrimental effects on their visual quality. These results are important considering the nutraceutical value of this plant and its wide commercial distribution

Myocarditis and intramural coronary vasculitis in polyarteritis nodosa: an unusual treatable form of heart failure

We describe an uncommon cardiac presentation of polyarteritis nodosa. A 68-year-old woman, with a history of fatigue, weight loss, and myalgia of the lower extremities, was admitted for congestive heart failure. Coronary arteries were normal. Endomyocardial biopsy showed active lymphocytic myocarditis with associated intramural small vessels necrotizing vasculitis. The overexpression of TLR-4 and the negativity for myocardial viruses suggested an immune mediated mechanism of cardiac damage. These histologic findings associated to weight loss >4 kg not due to dieting or other factors, myalgias, and polyneuropathy, were consistent with the diagnosis of polyarteritis nodosa. Immunosuppressive treatment, consisting of cyclophosphamide and prednisolone, led to a significant improvement of cardiac function. Polyarteritis nodosa can be the cause of unexplained heart failure due to myocarditis and intramural vessels vasculitis. Its recognition is crucial to obtain a cardiac recovery with a tailored immunosuppressive treatment

Application of Vis/Nir spectroscopy to establish peach ripening as affected by rootstock

The objective of this paper was to monitor peach ripeness of “Chimarrita” and “Maciel” cultivars, grafted on different rootstocks, using the pulp firmness parameter, as an indicator of harvest time through equipment based on Vis/Nir spectroscopy. The orchard was installed in 2005, has a "V" conduction system with spacing of 5.0 x 1.5 m, and the cultivars are grafted on seven rootstocks: “Capdeboscq”, “Flordaguard”, “Nemaguard”, “Okinawa”, “Tsukuba”, “Umezeiro” and “Viamão”. After harvesting, the fruits were evaluated by the NIR CASE spectrophotometer, establishing categories of pulp firmness, between 40N and 60N for fruits consumed in a long term and <40N for immediate consumption. The analyzed rootstocks alter the peach ripeness of the “Chimarrita” and “Maciel” cultivars. The “Umezeiro” rootstock anticipates harvest for the “Chimarrita” cultivar. The “Nemaguard”/ “Maciel” combination provides fruits with a superior harvest period than the other ones evaluated. The Vis/Nir Spectroscopy is a useful tool to monitor the harvest of “Chimarrita” and “Maciel” cultivars

Age- and season-dependent pattern of flavonol glycosides in Cabernet Sauvignon grapevine leaves

Flavonols play key roles in many plant defense mechanisms, consequently they are frequently investigated as stress sensitive factors in relation to several oxidative processes. It is well known that grapevine (Vitis vinifera L.) can synthesize various flavonol glycosides in the leaves, however, very little information is available regarding their distribution along the cane at different leaf levels. In this work, taking into consideration of leaf position, the main flavonol glycosides of a red grapevine cultivar (Cabernet Sauvignon) were profiled and quantified by HPLC–DAD analysis. It was found that amount of four flavonol glycosides, namely, quercetin-3-O-galactoside, quercetin-3-O-glucoside, kaempferol-3-O-glucoside and kaempferol-3-O-glucuronide decreased towards the shoot tip. Since leaf age also decreases towards the shoot tip, the obtained results suggest that these compounds continuously formed by leaf aging, resulting in their accumulation in the older leaves. In contrast, quercetin-3-O-glucuronide (predominant form) and quercetin-3-O-rutinoside were not accumulated significantly by aging. We also pointed out that grapevine boosted the flavonol biosynthesis in September, and flavonol profile differed significantly in the two seasons. Our results contribute to the better understanding of the role of flavonols in the antioxidant defense system of grapevine

Tradeoff between Biomass and Flavonoid Accumulation in White Clover Reflects Contrasting Plant Strategies

An outdoor study was conducted to examine relationships between plant productivity and stress-protective phenolic plant metabolites. Twenty-two populations of the pasture legume white clover were grown for 4½ months during spring and summer in Palmerston North, New Zealand. The major phenolic compounds identified and quantified by HPLC analysis were glycosides of the flavonoids quercetin and kaempferol. Multivariate analysis revealed a trade-off between flavonoid accumulation and plant productivity attributes. White clover populations with high biomass production, large leaves and thick tap roots showed low levels of quercetin glycoside accumulation and low quercetin:kaempferol ratios, while the opposite was true for less productive populations. The latter included stress-resistant ecotypes from Turkey and China, and the analysis also identified highly significant positive relationships of quercetin glycoside accumulation with plant morphology (root:shoot ratio). Importantly, a high degree of genetic variation was detected for most of the measured traits. These findings suggest merit for considering flavonoids such as quercetin as potential selection criteria in the genetic improvement of white clover and other crops