Solid Freeform Fabrication of Functional Silicon Nitride Ceramics by Laminated Object Manufacturing 1

The processing of silicon nitride (Si3N4) structural ceramics by Laminated Object Manufacturing (LOM) using ceramic tape preforms was investigated. The key processing stages involved green shape formation (which used the LOM process), followed by the burnout of all organics, and final densification by pressureless sintering. Two material systems were considered. These were a) monolithic Si3N4 and b) a preceramic polymer infiltrated Si3N4. The raw materials for the process were tape preforms of Si3N4, which were fabricated by standard tape casting techniques. Mechanical property data obtained for the LOM processed Si3N4 showed high strength and fracture toughness values. The room temperature and high temperature (1260 o C) flexural strengths were in the range of 700-900 MPa and 360-400 MPa, respectively. The fracture toughness averaged from 5.5-7.5 MPa.m1/2. These strength and fracture toughness values are comparable to those reported for conventionally prepared Si3N4 ceramics. Thus, this research demonstrated that the LOM technique is a viable method for preparing functional Si3N4 ceramics with good physical and mechanical properties.Mechanical Engineerin

Studies on the Geology and Beneficiation of Chandak Magne-site Deposit from Pithoragarh District, Uttar Pradesh

Stringent operating conditions in modern steel making furnaces demand high quality basic refractories based on magnesia. Source of good quality natural magnesite cont-ains considerable amounts of SiO2 and fluxing agents. Therefore, appropriate measures are to be taken to ensure the availablity of good quality magnesite. Measures for meeting the demand of quality magnesite are follows:- i) Upgrading of the presently available natural magnesite by various beneficiation techniques ; ii) Production of high quality magnesia from sea water

Pathological studies of Glaeosporium psidii causing die-back of guavas

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Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)

This report describes the cloning and expression of a gene lycopene epsilon cyclase, (LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was identified from an SSH library constructed for quality trait in tea. 5’ and 3’ RACE (rapid-amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology studies showed that the deduced amino acid sequence of LCYE gene had the highest sequence identity of up to 84% with Vitis vinefera. The cloned gene was successfully expressed in a PET based Escherichia coli expression system. The size of the expressed protein was 59615 Daltons. A suppression subtractive library was constructed using a quality clone H3111 (tester) and a garden series clone T3E3 (driver).Key words: Carotenoid, RACE, heterologous expression, lutein, tea

Novel Gene Acquisition on Carnivore Y Chromosomes

Despite its importance in harboring genes critical for spermatogenesis and male-specific functions, the Y chromosome has been largely excluded as a priority in recent mammalian genome sequencing projects. Only the human and chimpanzee Y chromosomes have been well characterized at the sequence level. This is primarily due to the presumed low overall gene content and highly repetitive nature of the Y chromosome and the ensuing difficulties using a shotgun sequence approach for assembly. Here we used direct cDNA selection to isolate and evaluate the extent of novel Y chromosome gene acquisition in the genome of the domestic cat, a species from a different mammalian superorder than human, chimpanzee, and mouse (currently being sequenced). We discovered four novel Y chromosome genes that do not have functional copies in the finished human male-specific region of the Y or on other mammalian Y chromosomes explored thus far. Two genes are derived from putative autosomal progenitors, and the other two have X chromosome homologs from different evolutionary strata. All four genes were shown to be multicopy and expressed predominantly or exclusively in testes, suggesting that their duplication and specialization for testis function were selected for because they enhance spermatogenesis. Two of these genes have testis-expressed, Y-borne copies in the dog genome as well. The absence of the four newly described genes on other characterized mammalian Y chromosomes demonstrates the gene novelty on this chromosome between mammalian orders, suggesting it harbors many lineage-specific genes that may go undetected by traditional comparative genomic approaches. Specific plans to identify the male-specific genes encoded in the Y chromosome of mammals should be a priority