Growth Hormone Gene Polymorphism in Four Cattle Breeds in Turkey

This study was conducted to determine DNA-polymorphism of a AluI RFLP at bovine growth hormone (bGH) gene in Zavot (n=48), East Anatolian Red (n=40), Simmental (n=94) and Brown Swiss (n=64) cattle breeds. A total of 246 cattle were genotyped for the bGH-AluI polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the study, two alleles (L and V) and three genotypes (LL, VV and LV) were revealed after than digestion of amplification product with AluI restriction enzyme. Allelic frequencies for EAR, SIM, BS and Zavot breeds were determined as 0.775, 0.734, 0.781 and 0.760 respectively for L allele; 0.225, 0.266, 0.219 and 0.240 respectively for V allele. Otherwise, genotypic frequencies were 0.65, 0.57, 0.63 and 0.63 for LL, 0.10, 0.11, 0.06 and 0.10 for VV, and 0.25, 0.32, 0.31 and 0.27 for LV respectively. A significant deviation from Hardy-Weinberg equilibrium was not observed in the investigated breeds. As a result, this study provided information on the polymorphism of bGH in four cattle breeds. Additionally, this study reported the existence of a genetic polymorphism at bGH gene in Zavot cattle breed for the first time

Detection of Bovine Leukocyte Adhesion Deficiency (BLAD) Allele in Holstein Cows Reared in Kayseri Vicinity

The purpose of this work was to study whether the bovine leukocyte adhesion deficiency (BLAD) allele was present in the Holstein cows reared in Kayseri vicinity. Blood samples were obtained from 136 Holstein cows. In order to determine the area of mutation in PCR products, the PCR products were digested with TaqI endonuclease enzyme. It was found that three of the 136 Holstein cows were BLAD carriers. The mutant BLAD allele frequency in the 136 Holstein cows was calculated as 2.2%.

EFFECTS OF DGAT1 AND GH1 POLYMORPHISM ON MILK YIELD IN HOLSTEIN COWS REARED IN TURKEY

The aim of this study was to analyse the associations among the K232A polymorphism in the diacylglycerol acyltransferase1 (DGAT1) gene and L127V polymorphism in the bovine growth hormone (GH1) gene and milk yield in Holstein cows. A total of 281 first and second lactation Holstein cows from three different dairy cattle farms in the Burdur and Kayseri provinces of Turkey were included in this study. The PCR-RFLP method was used for DGAT1 and GH1 genotyping. The frequencies of genotypes and alleles of GH1 gene were found to be 0.78 for LL, 0.18 for LV and 0.04 for VV; 0.87 for the L allele and 0.13 for the V allele. The frequencies of genotypes and alleles of the DGAT1 gene were to be 0.61 for AA, 0.30 for KA and 0.09 for KK; 0.76 for the A allele and 0.24 for the K allele. No relations were found between DGAT1-K232A genotypes and the average milk yield in the first three lactations. However, an association between GH1-L127V polymorphism and the average milk yield of Holstein cows was found in the first three lactations. Cows with LL genotype had higher milk yield compared to LV and VV cows (P<0.05)

Effect of Different Gonadotropin Preparation on Ovulatory Response and Embryo Yield in Karayaka Ewes

WOS: 000307911300024In this study, the main goal was to investigate the effect of stimulation with either oFSH (Ovagen (R)) or pFSH (Folltropin (R)) on the superovulatory response and embryo quality in Karayaka ewes. Thirty-five ewes were used in this study. The ewes were randomly allocated to two groups. Estrous cycles in ewes were synchronized with the controlled integral drug release dispensers (CIDR (R)) for 14 days. Starting on day 12 and during 4 consecutive days ewes were treated with oFSH in Group I (GI, n=16) and pFSH in Group II (GII, n=19). Ovarian examination and embryo recoveries were carried out with the aid of laparotomy on day 6 following CIDR withdrawal. There was no significant differences in the number of corpora lutea (CL), unfertilized oocytes (UFOs), total number of embryos (EMBTot), total number of freezable embryos (FEMBTot), ovulation rate (OR), fertilization rate (FR), recovery rate (RR) and embryo recovery rate (ERR) between the groups. On the other hand, embryo quality was better in GI compared to GII (P<0.05). In conclusion, it has been thought that application of oFSH for superovulatory treatment in Karayaka ewes was more effective than pFSH for the quality of embryos

Genetic Polymorphism of Five Genes Associated with Meat Production Traits in Five Cattle Breeds in Turkey

There are many potential genes that are known to be related to meat production and that can be used for selection to improve meat quality and production include Calpain (CAPN1), Myostatin (MSTN), Calpastatin (CAST), Osteopontin (SPP1) and Thyroglobulin (TG). This study evaluated Zavot (n=60), Anatolian Black (n=59; AB), South Anatolian Red (n=53; SAR), Turkish Gray (n=60; TG) and East Anatolian Red (n=49; EAR) cattle breeds, with the primary goal of investigating DNA polymorphisms of the CAST, TG, SPP1, MSTN and CAPN1 genes. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotypes. Restriction enzymes revealed polymorphisms with 2 alleles and three genotypes of each CAST, TG, SPP1 and CAPN1 genes were determined in all breeds, while the MSTN locus was found to be monomorphic. Deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the five cattle breeds on SPP1 locus. Significant deviation was observed from HWE in the TG and AB breeds on TG locus. Except for the TG and SAR breeds, genotype frequencies were not consistent with the HWE on CAPN1 locus. Four breeds were found at Hardy-Weinberg equilibrium except AB breed for CAST locus. Consequently, this study has shown that genetic polymorphisms do not exist in the MSTN gene but do exist in the remaining four genes that were examined in five Turkish native cattle breeds

Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

The melatonin receptor 1A (MTNR1A) and inhibin alpha subunit (INHA) genes play a significant role in the reproductive characteristics of animals. Blood samples were collected from 371 goats (Honamli and Hair) reared in Antalya and Burdur. The polymerase chain reaction (PCR) products were digested by RsaI for the MTNR1A gene and HaeII for the INHA gene. Two alleles (A and G) and three genotypes (AA, AG, and GG) were observed for the INHA gene, while two alleles (R and r) and two genotypes (RR and Rr) were observed for the MTNR1A gene. The highest allelic frequency value for G (91.8%) was found in Honamli goat breeds for the INHA gene while the highest value for R (98.1%) was found in Hair goat breeds for the MTNR1A gene. The GG genotype for the INHA gene and the RR genotype for the MTNR1A gene were identified as the most common genotypes of the Honamli and Hair goat breeds. The rr genotype for the MTNR1A gene could not be determined in the breeds. Both Honamli and Hair goat breeds were in Hardy-Weinberg equilibrium for the genes that were studied. In conclusion, this study confirms the existence of genetic polymorphism in the MTNR1A and INHA genes as detected by PCR-RFLP analysis in Honamli and Hair goat breeds

Identification of beta-Lactoglobulin Gene SacII Polymorphism in Honamli, Hair and Saanen Goat Breeds Reared in Burdur Vicinity

This study was conducted to determine DNA-polymorphism of a SacII RFLP at exon 7 of the beta-lactoglobulin (beta-LG) gene in Honamli (31), Hair (39) and Saanen (41) goat breeds. A total of 111 goats were genotyped for the beta-lactoglobulin-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the studied breeds, digestion of amplification product with SacII restriction enzyme revealed two alleles namely, A and B and three genotypes (AA, BB and AB). Allelic frequencies for Hair, Saanen and Honamli breeds were 0.42, 0.37 and 0.53 respectively for A allele; 0.58, 0.63 and 0.47 respectively for B allele, while genotypic frequencies were 0.13, 0.12 and 0.19 for AA, 0.28, 0.39 and 0.13 for BB, and 0.59, 0.49 and 0.68 for AB respectively. Deviation from Hardy-Weinberg equilibrium was not observed in the studied breeds. As a result, this study provided information on the polymorphism of beta-lactoglobulin in three goat breeds. Additionally, this study reported the existence of a genetic polymorphism at beta-LG gene in Honamli goat breed for the first time