Determinación de la identidad de la variedad 'Pedro Giménez' cultivada en Argentina a través del empleo de marcadores microsatélites

Pedro Giménez' is a white criolla variety cropped in Argentina, mainly in Mendoza and San Juan, being the most planted white variety destined for wine making in the country. Its origin remains unknown, as well as its relationship with Spanish variety 'Pedro Ximénez', mostly grown in Jerez, Spain. Previous works have probed that most of Criollas varieties existing in America at the moment, are the offspring of 'Muscat of Alexandria' x 'Criolla Chica'. The aim of the present work was to compare 'Pedro Giménez' with the Spanish variety 'Pedro Ximénez', and to establish its degree of relatedness to 'Muscat of Alexandria' and 'Criolla Chica'. Therefore we used a set of 18 nuclear SSR loci and 3 chloroplast SSR loci. 'Pedro Giménez' shared only 38% of the alleles under analysis with 'Pedro Ximénez', indicating that they are indeed two different varieties. In all 18 polymorphic nuclear SSR loci 'Pedro Giménez' shared 50% of its alleles with 'Muscat of Alexandria', while the other 50% of the alleles present in 'Pedro Giménez' were also present in 'Criolla Chica'. This data, along with those from the chloroplast SSR analysis, strongly suggest that 'Pedro Giménez' is the progeny of 'Muscat of Alexandria' x 'Criolla Chica', being the latest one the most likely female progenitor.'Pedro Giménez' es una variedad criolla blanca cultivada en Argentina, principalmente en las provincias de Mendoza y San Juan, siendo la variedad con la mayor superficie entre las uvas blancas de vinificación. Su origen es desconocido, como también su relación con la variedad española 'Pedro Ximénez', cultivada especialmente en la región de Jerez, España. En trabajos previos se ha probado que la mayoría de las variedades criollas existentes en América se habrían originado del cruzamiento de 'Moscatel de Alejandría' x 'Criolla Chica'. El presente trabajo tuvo como objetivos comparar las variedades 'Pedro Giménez' y 'Pedro Ximénez', y establecer relaciones de parentesco con 'Moscatel de Alejandría' y 'Criolla Chica'. Se utilizaron 18 loci microsatélites nucleares y 3 loci microsatélites de cloroplasto. 'Pedro Giménez' compartió sólo el 38% de los alelos con 'Pedro Ximénez', por lo que se pudo inferir que se trata de variedades diferentes. En todos los loci polimórficos nucleares analizados 'Pedro Giménez' compartió uno de los alelos con 'Moscatel de Alejandría' y el otro con 'Criolla Chica'. Estos datos, junto con el resultado del análisis de SSR de cloroplastos, avalan la hipótesis que, como muchas de las variedades criollas, 'Pedro Giménez' sería fruto del cruzamiento entre estos dos progenitores, siendo 'Criolla Chica' probablemente el progenitor materno.Fil: Durán, Martín F.. Universidad Nacional de Cuyo. Facultad de Ciencias AgrariasFil: Agüero, Cecilia.Fil: Martínez, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Agraria

Convenio tripartito entre la Universidad, la Dirección Penal Juvenil y la Secretaría de recursos hídricos en salta experiencias en transferencia y producción de tecnología.

Se presentan las tareas realizadas que condujeron a la concreción de un convenio entre tres partes institucionales de la Provincia de Salta. El objetivo principal fue la transferencia y producción de tecnología sencilla para la cocción solar y de cocinas a leña optimizadas, con jóvenes alojados en dispositivos dependientes de la Dirección General de Justicia Penal Juvenil, a fin de satisfacer necesidades de personas originarias de la margen salteña del río Pilcomayo.Fil: Passamai, Victor Jose. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Salta. Instituto de Invest.en Energia No Convencional; Argentina;Fil: Fernández, S.. Ministerio de Infraestructura y Vivienda Serv.pub.. Secretaria de Obras Publicas. Subsecretaria de Recursos Hidricos; Salta; Argentina;Fil: Cali, S.. Ministerio de Infraestructura y Vivienda Serv.pub.. Secretaria de Obras Publicas. Subsecretaria de Recursos Hidricos; Salta; Argentina;Fil: Agüero, M.. Dirección Penal Juvenil. Salta; Argentina;Fil: Teruel, F.. Dirección Penal Juvenil. Salta; Argentina

Vacuum tribological behaviour of self lubricant quasicrystalline composite coatings

High temperature resistant self-lubricant coatings are needed in space vehicles for components that operate at high temperatures and/or under vacuum. Thick composite lubricant coatings containing quasicrystalline alloys (QC) as the hard phase for wear resistance, have been deposited by thermal spray. The coatings also comprise lubricating materials (silver and BaF2-CaF2 eutectic) and NiCr as the tough component. This paper describes the vacuum tribological properties of TH103, a coating belonging to this family, with excellent microstructural quality. The coating was deposited by HVOF and tested under vacuum on a pin-on-disc tribometer. Different loads, linear speeds and pin materials were studied. The pin scars and disc wear tracks were characterized by EDS-SEM. A minimum mean steady friction coefficient of 0.32 was obtained employing a X-750 Ni superalloy pin in vacuum conditions under 10 N load and 15 cm/s linear speed, showing moderate wear of the disc and low wear of the pin

Seasonal pattern of the fish assemblage of El Conchalito mangrove swamp, La Paz Bay, Baja California Sur, Mexico

The seasonal changes in fish assemblage structure and the importance of El Conchalito mangrove swamp was assessed from October 1996 to August 1997. A modified stationary flume net was deployed at the swamp channel just before high tide and operated continuosly until the neap tide during the full moon. A total of 35,138 fish specimens were caught, belonging to 34 species included in 26 genera and 16 families. The fish assemblage changed with seasons, the fish assemblage had the highest species richness (21) and highest relative dominance index (0.77) in summer, but the highest Shannon diversity (2.7 bits/ind.) and Pielou evenness (0.73) in winter. Fish assemblages of summer and autumn had the highest similarity (68%), which reflects environmental changes that drives a sequential pattern in community structure between summer-autumn and autumn-winter clusters. Dominant species also showed a seasonal alternation between Eucinostomus currani (autumn), Albula nemoptera (winter) and Anchoa ischana (spring and summer) by numbers, whereas Diapterus peruvianus (autumn), Mugil curema (winter), Sphoeroides annulatus (spring) and A. ischana (summer) were the dominant species by biomass. The structural analysis of the fish community revealed four dominant species, seven common species and 23 rare species. The importance of the mangrove area of El Conchalito estuary is demonstrated based on the diversity of marine fishes that use this ecosystem as primary habitat for nursery, foraging and recruitment areas, and by its low disturbed conditions

Update of Genetic Diversity of Porcine Circovirus Type 2 in Chile Evidences the Emergence of PCV2d Genotype.

Porcine Circovirus 2 (PCV2) can cause multiple clinical conditions known as porcine circovirus-associated diseases (PCVAD). Before the wide availability of PCV2 vaccines, PCVAD resulted in significant losses to the global swine industry. PCV2's rapid evolutionary dynamics are comparable to single-stranded RNA viruses. Thus, shifts in the dominance and distribution of different genotypes may frequently occur, resulting in the emergence and spread of varying PCV2 genotypes and recombinant strains in swine. This study aims at identifying the PCV2 genotypes currently circulating in Chile. Seven hundred thirty-eight samples were obtained from 21 swine farms between 2020 and 2021. The samples were tested using PCR for species detection and genotyping. Sequencing and phylogenetic analyses were conducted in selected samples. PCV2 was detected in 26.9% of the PCR reactions and 67% of the sampled farms. The genotypes were determined in nine farms, PCV2a in one farm, PCV2b in four, and PCV2d in five, with PCV2b and PCV2d co-circulating in one farm. The phylogenetic analysis of twelve ORF2 sequences obtained (PCV2a = 5; PCV2b = 4; PCV2d = 3) showed a PCV2a Chilean strains monophyletic cluster; closely related to Chilean viruses collected in 2012 and 2013. Of the three different PCV2b sequenced viruses, two viruses were close to the root of the PCV2b group, whereas the remaining one grouped with a South Korean virus. PCV2d sequences were closely related to Asian viruses. A previously reported PCV2a/PCV2d recombinant strain was not detected in this study. Our results suggest the emergence and potential shift to PCV2d genotype in Chilean farms

Vacuum Tribological Behaviour of Self-Lubricating Quasicrystalline Composite Coatings

High-temperature-resistant self-lubricating coatings are needed in space vehicles for components that operate at high temperatures and/or under vacuum. Thick composite lubricant coatings containing quasicrystalline alloys as the hard phase for wear resistance can be deposited by a thermal spray technique. The coatings also contain lubricating materials (silver and BaF2CaF2 eutectic) and NiCr as the tough component. This paper describes the vacuum tribological properties of TH103, a coating of this type, with a very good microstructural quality. The coating was deposited by high-velocity oxygen fuel spraying and tested under vacuum using a pin-on-disc tribometer. Different loads, linear speeds, and pin materials were studied. The pin scars and disc wear tracks were characterised using a combination of scanning electron microscopy and energy dispersive spectrometry. A minimum mean steady friction coefficient of 0.32 was obtained when employing an X750 Ni superalloy pin in vacuum conditions under 10 N load and 15 cm/s linear speed, showing moderate wear of the disc and low wear of the pi

The Merging System Am 2049-691

Spectroscopic and photometric observations of the peculiar object AM 2049-691 are presented here. Its systemic velocity is V(GSR) = (10956 +-30) km/s, and the derived distance (H(0) = 75 km/s/Mpc) results 146 Mpc. A bridge is observed between two very distinct nuclei whose separation is about 10 kpc, as well as two tails that emerge from the extremes SW and NE of the main body and extend up to 41 and 58 kpc respectively. The spectral characteristics of the all observed zones are typical of H II regions of low excitation. The internal reddening is quit high, particularly in the NE nucleus. All the derived equivalent widths of the H(alpha)+[N II] lines indicate enhanced star formation compared with isolated galaxies, specially in the NE nucleus; the equivalent width corresponding to the integrated spectrum reflects starburst activity in the whole object, and is compatible with a merger of two disk galaxies. All the observed characteristics of AM 2049-691 indicate it is a merger, where a overabundance of nitrogen is detected in one of the nuclei, which has the most evolved population and would be the most massive one. The detected total IR emission is not very high. The integrated total color B - V corresponds to a Sc-Scd galaxy and its average integrated population is about F7 type. Indicative B - V colors of the nuclei, corrected for internal absorption, are in agreement with the spectroscopic results. The central radial velocity dispersions at the nuclei suggest that the most massive galaxy would be the progenitor of the SW component. The observed radial velocity curve shows the presence of two subsystems, each one associated with a different nucleus.Comment: 5 pages, 4 figure

Estimation of renal function by CKD-EPI versus MDRD in a cohort of HIV-infected patients: a cross-sectional analysis

Background: Accurately determining renal function is essential for clinical management of HIV patients. Classically, it has been evaluated by estimating glomerular filtration rate (eGFR) with the MDRD-equation, but today there is evidence that the new Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation has greater diagnostic accuracy. To date, however, little information exists on patients with HIV-infection. This study aimed to evaluate eGFR by CKD-EPI vs. MDRD equations and to stratify renal function according to KDIGO guidelines. Methods: Cross-sectional, single center study including adult patients with HIV-infection. Results: Four thousand five hundred three patients with HIV-infection (864 women19%) were examined. Median age was 45 years (IQR 37-52), and median baseline creatinine was 0.93 mg/dL (IQR 0.82-1.05). A similar distribution of absolute measures of eGFR was found using both formulas (p = 0.548). Baseline median eGFR was 95.2 and 90.4 mL/min/ 1.73 m(2) for CKD-EPI and MDRD equations (p 90 mL/min/1.73 m(2)) in 73% patients and '' mild reduced GFR '' (60-89 mL/min/1.73 m(2)) in 24.3% of the patients, formerly classified as >60 mL/min/1.73 m(2) with MDRD. Conclusions: There was good correlation between CKD-EPI and MDRD. Estimating renal function using CKD-EPI equation allowed better staging of renal function and should be considered the method of choice. CKD-EPI identified a significant proportion of patients (24%) with mild reduced GFR (60-89 mL/min/1.73 m(2)).Red de SidaInstituto de Salud Carlos III, Madrid, SpainEuropean Regional Development Fund (ERDF)Univ Fed Sao Paulo, Kidney Transplant Div, Hosp Rim, Sao Paulo, BrazilUniv Barcelona, Hosp Clin IDIBAPS, Nephrol & Renal Transplantat Serv, Barcelona, SpainHosp Clin Barcelona, Biomed Diag Ctr, Core Lab, Barcelona, SpainHosp Olot, Internal Med Serv, Girona, SpainUniv Girona, Dept Med Sci, Girona, SpainUniv Barcelona, Hosp Clin IDIBAPS, Infect Dis Serv, Barcelona, SpainUniv Fed Sao Paulo, Kidney Transplant Div, Hosp Rim, Sao Paulo, BrazilRed de Sida: RD12/0017/0001Instituto de Salud Carlos III, Madrid, Spain: INT15/00168Web of Scienc

traT gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of Escherichia coli and other gram-negative bacteria

The R6-5 plasmid-specified outer membrane protein, TraT protein, has previously been shown to mediate resistance to bacterial killing by serum. Colony hybridization with a 700 bp DNA fragment carrying most of the traT gene was used to examine the prevalence of traT in Gram-negative bacteria, particularly strains of Escherichia coli, isolated from clinical specimens. traT was found in isolates of E. coli, Salmonella, Shigella and Klebsiella, but not in Pseudomonas, Aeromonas or Plesiomonas, nor in the few isolates of Enterobacter, Proteus, Acinetobacter, Citrobacter, Serratia or Yersinia that were examined. It was detected in a significantly higher proportion of the E. coli strains isolated from the blood of patients with bacteraemia/septicaemia or from faeces of patients with enteric infections (50-70%) than in that of strains isolated from normal faeces (20-40%). The incidence of traT in strains isolated from cases of urinary tract infections was variable. traT was found to be frequently associated with production of the K1 capsule and with the carriage of ColV plasmids, but not with the carriage of R plasmids, nor with serum resistance or the production of haemolysin