Antibiotics Resistance of Vibrio spp. Isolated from Diseased Seabass and Tilapia in Cage Culture

Vibriosis has become one of the most important bacterial diseases in marine cultured organisms in recent years. This study was focusing on isolation and identification of Vibrio spp. isolated from diseased seabass (Lates calcarifer), tilapia (Oreochromis niloticus) and seawater from Sri Tujuh lagoon in East Coast of Malaysia; also determination of antibiotic resistance patterns among Vibrio spp. Vibrio species isolated from diseased seabass in Pantai Sri Tujuh, Tumpat, Kelantan were screened for their antibiotic sensitivity patterns by Kirby-Bauer method. A total of 47 isolates belonging to three different species were identified which are V. parahaemolyticus, V. vulnificus, and V. alginolyticus. In this study, high incidence of erythromycin, chloramphenicol and sulfamethoxazole resistance was observed among the Vibrio isolates, whereas all isolates were susceptible to oxytetracycline. Vibrio isolates were 96% resistant to one or more different classes of antibiotic, and 17 different resistance patterns were identified. The MAR index of 0.4 indicating the Vibrio spp. in these farmed fish might have been indiscriminately and continuously exposed to those antibiotics during culturing stages of the fish. This study showed that multidrug-resistant Vibrio spp. were common in diseased seabass and tilapia cultured at Sri Tujuh lagoon. These essential findings suggested involvement of seafood in transmission of these pathogen to human. In addition, oxytetracycline can be used as a treatment to combat vibriosis in diseased seabass and tilapia

The Influence of Role Playing Learning Model on Learning Outcomes of Speaking Skills in Simple Interviewing for Fifth Grade Students of SDN 2 Tambakrigadung Lamongan

The purpose of this study is to see how the role playing learning model affects students' speaking skills in class 5C SDN 2 Tambakrigadung Lamongan. This is a quantitative study that use the Posttest-Only Control Design approach. The data was collected using a random sampling technique and a learning outcome test in the form of a description (conversational text). The two-sample paired t-test formula was used for hypothesis testing. With 39 students in each class, the sample comprised of two classes: class 5C as the experimental group and class 5A as the control group. The results of the data analysis revealed that the role playing model had an effect on the speaking abilities of 5th grade students at SDN 2 Tambakrigadung Lamongan.The results of the data analysis revealed that the value of tcount was bigger than ttable, or 5.80 > 1.665 at a significance level of 5%, hence H0 was rejected and Ha was given

Public Awareness and Practices Towards Self-Medication with Antibiotics Among Malaysian Population: Questionnaire Development and Pilot Testing

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