Ethynylbenziodoxolone Reactivity in Cysteine Bioconjugation

Selective labeling of cysteine is crucial to enable the study of the many biological processes in which cysteine plays a key part in the structure and activity of proteins. In this issue of Chem, Waser, Fierz, and co-workers describe an efficient, chemoselective cysteine bioconjugation method to introduce vinylbenziodoxolone into biomolecules

International Veterinary Epilepsy Task Force consensus proposal: Medical treatment of canine epilepsy in Europe

In Europe, the number of antiepileptic drugs (AEDs) licensed for dogs has grown considerably over the last years. Nevertheless, the same questions remain, which include, 1) when to start treatment, 2) which drug is best used initially, 3) which adjunctive AED can be advised if treatment with the initial drug is unsatisfactory, and 4) when treatment changes should be considered. In this consensus proposal, an overview is given on the aim of AED treatment, when to start long-term treatment in canine epilepsy and which veterinary AEDs are currently in use for dogs. The consensus proposal for drug treatment protocols, 1) is based on current published evidence-based literature, 2) considers the current legal framework of the cascade regulation for the prescription of veterinary drugs in Europe, and 3) reflects the authors’ experience. With this paper it is aimed to provide a consensus for the management of canine idiopathic epilepsy. Furthermore, for the management of structural epilepsy AEDs are inevitable in addition to treating the underlying cause, if possible

Ethynylbenziodoxolone Reactivity in Cysteine Bioconjugation

Selective labeling of cysteine is crucial to enable the study of the many biological processes in which cysteine plays a key part in the structure and activity of proteins. In this issue of Chem, Waser, Fierz, and co-workers describe an efficient, chemoselective cysteine bioconjugation method to introduce vinylbenziodoxolone into biomolecules

Serine-Selective Bioconjugation

This Communication reports the first general method for rapid, chemoselective, and modular functionalization of serine residues in native polypeptides, which uses a reagent platform based on the P(V) oxidation state. This redox-economical approach can be used to append nearly any kind of cargo onto serine, generating a stable, benign, and hydrophilic phosphorothioate linkage. The method tolerates all other known nucleophilic functional groups of naturally occurring proteinogenic amino acids. A variety of applications can be envisaged by this expansion of the toolbox of site-selective bioconjugation methods