8 research outputs found

    AFLPs Raw Data

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    AFLPs Raw data matrix. This data were obtained from the presence or absence of band in the polyacrylamide gels. 111 isolates were includes and 57 band were scored. These data were implemented to generate the distance tree and the Minimum Spanning Network using SplitsTree version 4.12.3 and BioNumerics software (version 7.1) respectively

    NJ_VNTR_bootstrap_llanos_Xam

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    VNTR data were used to created an unrooted distance tree with the Neighbor-Joining algorithm in SplitsTree version 4.12.3. One thousand bootstrap replicates were run

    VNTR Raw data

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    This file contains the numbers of the repeats obtained from the sequenced VNTR loci. These data were implemented to generated the Minimum Spanning Network using BioNumerics software (version 7.1

    VNTR Structure project

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    This file contains the STRUCTURE project generated using the repeats of the sequenced VNTRs. Xam isolates were assigned into genetic populations using a clustering algorithm based on Bayesian model in STRUCTURE 2.3.3 without prior population information. Genetic clusters of the isolates were generated with independent allele frequencies and five thousand replicates during burning period and 100.000 Monte Carlo Markov chain (MCMC) iteration

    NJ_AFLP_bootstrap_llanos_Xam

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    Unrooted distance tree with the Neighbor-Joining algorithm was generated in SplitsTree version 4.12.3. Branch supports were determined running 1000 bootstrap replicates

    AFLP Structure Project

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    This file contains the STRUCTURE project generated using the AFLP data. Xam isolates were assigned into genetic populations using a clustering algorithm based on Bayesian model in STRUCTURE 2.3.3 without prior population information. Genetic clusters of the isolates were generated with independent allele frequencies and five thousand replicates during burning period and 100.000 Monte Carlo Markov chain (MCMC) iterations

    Matrix_VNTR_llanos_Xam

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    Matrix generated by presence or absence of an allele in a specific VNTR locus. In total 5 VNTR loci were used. The allele number for each locus is variable and those are described in the table 1 of the manuscript

    <i>Physalis peruviana</i> responses to <i>Phytophthora infestans</i> are typical of an incompatible interaction

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    <div><p></p><p><i>Phytophthora infestans</i>, a hemibiotrophic and heterothallic oomycete pathogen, attacks a wide variety of crop plants belonging to the Solanaceae family. In 2007, late blight symptoms were observed and confirmed on <i>Physalis peruviana</i> in fields during a late blight survey in Colombia. Subsequent to these observations, no further symptoms have been detected in the field. This prompted an interest in investigating the interaction between <i>P. infestans</i> and <i>P. peruviana</i> using histological and molecular assays, as well as characterizing defence reactions produced on <i>P. peruviana</i> leaves. During this interaction, callose depositions, accumulation of hydrogen peroxide, activation of pathogenesis-related proteins (β-1,3 glucanase and peroxidase), induction of the expression of genes associated with plant defences (<i>glucanase A</i> and <i>carbonic anhydrase</i>) as well as cell death, showed that <i>P. peruviana</i> has active defences against <i>P. infestans</i> strains present in Colombia.</p></div
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