Photographic grading to evaluate facial cleanliness and trachoma among children in Amhara region, Ethiopia.

BACKGROUND: Promotion of facial cleanliness is recommended for the elimination of blinding trachoma, largely because of observational studies that have found an association between various measures of facial uncleanliness and trachoma. However, when a field grader assesses both facial cleanliness and trachoma, associations may be biased. Assessment of photographs of the face and conjunctiva by masked graders may provide a less biased estimate of the relationship between facial cleanliness and trachoma. METHODS: Face photographs, conjunctival photographs, and conjunctival swabs were obtained on a random sample of 0-9-year-old children from each of 40 communities in Amhara region, Ethiopia. Face photographs were assessed for the presence of seven measures of an unclean face (i.e., wet nasal discharge, dry nasal discharge, wet ocular discharge, dry ocular discharge, food, dust/dirt, and flies) by three independent masked photo-graders. Conjunctival photographs were similarly graded in a masked fashion for signs of clinically active trachoma. Conjunctival swabs were processed for Chlamydia trachomatis DNA. RESULTS: Of 2073 children with complete data, 808 (39%) had evidence of clinically active trachoma, 150 (7%) had evidence of ocular chlamydia infection, and 2524 (91%) had at least one measure of an unclean face. Dry ocular discharge had the strongest association with clinically active trachoma (age- and sex-adjusted prevalence ratio [PR] 1.4, 95% CI 1.2-1.6) and ocular chlamydia infection (PR 1.9, 95%CI 1.3-2.9), although significant associations were observed between each of the measures of facial uncleanliness and trachoma. CONCLUSIONS: Masked assessment of face and conjunctival photographs confirmed prior observational studies that have noted associations between various measures of facial uncleanliness and trachoma. The causal relationship between facial uncleanliness and trachoma is unclear since many features used to measure facial cleanliness (e.g., ocular discharge, nasal discharge, and flies) could be consequences of antecedent ocular chlamydia infection. TRIAL REGISTRATION: NCT02754583, clinicaltrials.gov

CANCER NANOTHERANOSTICS: A NEW PARADIGM OF SIMULTANEOUS DIAGNOSIS AND THERAPY

Despite  improvements  in  our  understanding  of cancer  and  the  emerging  concept  of personalized medicine for the treatment of this disease, it is still one of the leading causes of death worldwide. Although, in recent years, significant advances have been achieved in cancer therapy, many tumors are still challenging to treat and novel strategies are required to effectively combat this deadly disease. Nanotheranostics is a burgeoning field which makes use of nanotechnology for diagnosis and therapy of cancer. The recent advancement in the area of nanotechnology has enabled a new generation of different types of nanomaterials composed of either inorganic or polymer based nanoparticles to be useful for nanotheranostics applications such as to diagnose and treat diseases and monitoring the therapeutic response in vivo at molecular level; to enhance the control, evaluation and optimization of drug delivery and release; to target the drug by conjugating theranostic nanoplatformes with biological ligands. This review, therefore, summarizes the various nanocarriers developed so far for the simultaneous imaging and therapy, strategies for their targeted delivery, their potential applications and the challenges in their development and application for cancer therapy.   Key words: Cancer; Cancer therapy; Nanoparticles; Nanotheranostics

Genotype-environment interaction and stability analysis for grain yield of maize (Zea mays L.) in Ethiopia

Fifteen maize genotypes were tested at nine different locations in 2005 under rainfed condition to determine stable maize genotypes for grain yield and determine genotypes with high yield and form homogenous grouping of environments and genotypes. The experiment was conducted using Randomized Complete Block Design with three replications. There was considerable variation among genotypes and environments for grain yield. Stability was estimated using the Additive Main Effects and Multiplicative Interactions (AMMI). Based on the stability analysis, genotypes 30H83, BH-540, Ambo Synth-1, AMH-800 and BHQP-543 were found to be stable for grain yield. The first two Interaction Principal Component axis (IPCA1 and IPCA2) were significant (

Performance evaluation and selection of new maize hybrids under sole and inter crop production systems

Screening of maize genotypes under different cropping systems (sole and inter cropping) is very important to understand the genotypes response for different abiotic and a biotic stress. Nine maize genotypes including the standard check (BH-543) were planted and evaluated at research and farmers’ fields in the 2011 and 2012 cropping seasons. Farmers were invited to evaluate the genotypes based on their criteria of selection. Hawassa-Dume common bean variety was used for intercropping purpose in 2012. The genotypes substantially varied for yield and other traits both under sole and intercropping systems. When combined across seasons, the high yielding genotypes, genotype-1 and genotype-5 showed 38 and 37% yield advantage over the standard check. Besides, genotypes markedly varied for their compatibility for intercropping system with land equivalent ratio (LER) 1 highlighting the need to evaluate genotypes for intercropping system at early stage of breeding

Adapting agriculture to climate change in Ethiopia

Published by the Ethiopian Institute of Agricultural Research, this brochure raises awareness about actions that can be taken to reduce vulnerability to climate change and climate variability. Among other recommendations, it includes: developing institutional capacity to generate and apply climate information at the local level; development of rainfall risk insurance schemes; harmonization of policies and institutional frameworks affecting climate change adaptation; allocation of budgets for communication, sharing, and the scaling up of available climate change adaptation options

Performance Evaluation and Identification of New Quality Protein Maize Inbred Lines

Background: Maize plays a critical role in meeting high food demand. It is a globally widely adopted and cultivated crop. Hybrid and open-pollinated varieties development from fixed inbred lines is one of the strategies for the improvement of maize production. Compared with the world average, the national average maize yield is low in Ethiopia. According to this development and selection of promising germplasm has indispensable value for developing high-yielding maize varieties. The study consists of 21Quality Protein Maize (QPM) lines, two QPM testers lines, and one Conventional Maize (CM) line check (FS67). They were evaluated in RCBD with two replications at Ambo and Arsi-Negele. The objective was to identify new lines with good performance compared with released QPM checks and CM lines. Results: There is a significant difference between the lines for 28 traits in Ambo and 24 traits at Arsi-Negele. In combined mean performance analysis, the highest yielding line (L8) exceeded the mean of all line checks, mean QPM checks, CM line check (FS67), and best QPM line check (CML144) by 34.89%, 54.80%, 7.30%, and 25.31%, respectively for GY. The value of EPP ranged from 0.91 (L14) to 1.85 (L3) with an overall mean of 1.19). The highest yielding line (L8) had the 2nd highest EPP (1.63). Mean EPP of the top five QPM lines was less by 5.45%, 4.96%, 22.41%, and 6.41% compared with the mean of all checks, mean of QPM line checks, best QPM check, and CM line check, respectively. The high yielder line (L8) had a higher mean value than the mean of lines checks (CML144, CML159, and FS67), mean of QPM lines checks, best QPM check (CML144), and FS67 by 25.71%, 26.36%, 3.16%, and 24.43%, respectively. Conclusion: In general, the study confirmed the existence of promising new QPM parental lines. These promising lines can be used as source material in the breeding program for further improvement

Estimation of General and Specific Combining Ability Effects for Quality Protein Maize Inbred Lines

Background: Maize (Zea mays L.) plays a critical role in meeting high food demand. It is globally one of the most widely adapted and cultivated crops. Hybrid development from fixed inbred lines is one of the strategies for the improvement of maize production. The national average maize yield in Ethiopia is low and thus, selection of promising germplasm and knowledge of combining ability are prerequisites to developing high yielding maize varieties. Forty-two Quality Protein Maize (QPM) crosses (21 inbred lines each crossed with two testers) along with three popular standard hybrids were evaluated in two replications using alpha lattice during the 2017 cropping season at Ambo, Arsi-Negele, and Kulumsa. The objectives of this study were to identify lines with high GCA and estimate the SCA of crosses for grain yield, and other agronomic and morphological characters. Results: Significant difference among crosses was observed for 19 traits at Ambo, 14 traits at Arsi-Negele, and 19 traits at Kulumsa in the hybrid trial. Regarding the GCA effect, L8 had a significant difference to the positive side with the highest magnitude of GCA effect at three locations (3.40, 2.03, and 1.88 GCA effect values at Arsi-Negele, Ambo, and Kulumsa, respectively) which is followed by L7 for GY. All crosses did not show a significant SCA effect for GY in both directions at Ambo and Arsi-Negele but at Kulumsa, five crosses: L2xT1(1.89), L13xT2(1.88), L7xT1(1.86), L4xT2(1.49) and L19xT1(1.41) showed significant difference. In the combined analysis for six traits, Additive gene action was more important which was manifested by a higher sum square contribution of DS (79.6) EPP (79.3%), EL (80.0%), KPR (80.1%), ED (78.5%) and TSW (79.1%). Conclusion: Based on mean grain yield, and combining ability, L8xT2, L7xT1, L8xT1, L19xT1, L6xT2, and L18xT1 are promising crosses that could be forwarded for further use in maize breeding programs for further works