Morphophysiological Responses of Oat (Avena sativa L.) Genotypes from Pakistan’s Semiarid Regions to Salt Stress

Soil salinity is a major constraint to modern agriculture, with around 20% of the previously irrigated area becoming salt affected. Identifying suitable salt stress-tolerant genotypes based on their agronomic and physiological traits remains a herculean challenge in forage-type Oat (Avena sativa L.) breeding. The present study was designed to investigate the response of oat crop plants against the salt (NaCl) stress in Mardan, Pakistan. The experiment was carried out in complete randomized design (CRD) with two factors trail comprising of the performance of four different genotypes of oat (NARC oat, PARC oat, Green Gold and Islamabad oat) in response to four levels of saline stress (0, 25, 50 and 75 mmol L-1 NaCl). Plant growth and physiological parameters including germination (G, %); fresh shoot weight (FSW, g); fresh root weight (FRW, g); chlorophyll-a, chlorophyll-b, total chlorophyll, and total carotenoids were analyzed for identifying salt tolerance. Germination (%) of oat genotypes was negatively affected by higher salt stress. Mean values showed that maximum germination (57.5%) was recorded for control while minimum germination (48.75%) was recorded for 25 mmol L-1 NaCl and that maximum germination (58%) was recorded for PARC oat. The root and shoot fresh weight of all genotypes declined with increasing salt stress, while NARC and Green Gold oat showed considerably higher values than the other genotypes. Although chlorophyll and carotenoids were found to be negatively affected by increasing salt concentrations, NARC and Green Gold oat genotypes performed considerably better at 75 mmol L-1 NaCl when compared to the other genotypes. Based on the mean shoot dry weight ratio ± one standard error, the four Oat genotypes were categorized as salt-tolerant (Green Gold), moderately tolerant (PARC and NARC), and salt-sensitive (Islamabad). The more salt-tolerant genotype (Green Gold) demonstrated relatively high salinity tolerance and may be useful for developing high-yielding oat hybrids in future breeding programs under salt stress conditions

Zona survenu lors d’un traitement par Infliximab pour une Rectocolite hémorragique: Herpes Zoster following Infliximab therapy for Ulcerative colitis

The advent of biotherapy in particular tumor necrosis factor antagonists has revolutionized inflammatory bowel disease treatment. A risk of tuberculosis and opportunistic infections following treatment with anti-TNF-a has been well reported. However, little is known about the association between TNF-a blockers and viral infection. We report a case of an Ulcerative Colitis patient under azathioprine and infliximab who developed intercostal hyperalgic Herpes zoster without severity signs, having evolved well after association of analgesic with antiviral. Through our case, we explain physiopathological mechanisms. Indeed, Interferon-? and TNF-a play critical roles in the control of viral infection. Depletion of TNF-a by antiTNF-a treatment can facilitate reactivation of a viral infection, including herpes zoster. The risk of herpes zoster in patients with Ulcerative Colitis and treated with anti-TNF-a is frequent. Thus, the interest of a systematic and prophylactic treatment is raised. L’avènement de la biothérapie en particulier, des anti- TNF-a a révolutionné la prise en charge des maladies inflammatoires de l’intestin. Un risque de tuberculose et d’infections opportunistes a été bien signalé. Cependant, peu de données ont été rapportés sur l’association anti-TNF-a et infections virales. Nous rapportons le cas d’une rectocolite hémorragique sous azathioprine et infliximab atteint d’un zona intercostal hyperalgique sans signes de gravités ayant bien évolué après traitement antalgique et antiviral. A travers ce cas, les mécanismes physiopathologiques sont discutés. En effet, l’interféron-? et le TNF-a jouent un rôle critique dans le contrôle de l’infection virale. Ainsi, Leur épuisement par un blocage anti TNF peut faciliter la réactivation d’une infection virale notamment un risque accru de l’herpès zona. Le risque de zona chez les patients atteints d’une RCH et traités par anti-TNF est fréquent. Ce qui soulève l’intérêt d’un traitement prophylactique systématique

Partial Substitution of Chemical Fertilizers with Organic Supplements Increased Wheat Productivity and Profitability under Limited and Assured Irrigation Regimes

Crop wastes could be applied in conjunction with synthetic fertilizers to satisfy crop nutritional needs and enhance soil fertility. A field experiment was carried out during winter 2019–2020 at the AMK Research Farm (Palatoo) Mardan, KPK (Pakistan) to investigate the combined effect of phosphorous (PS) and organic sources (OSs) on wheat productivity under different irrigation regimes. The experimental factors were: two irrigation regimes (limited and full irrigation), three inorganic sources of phosphorus (triple super phosphate (TSP), single super phosphate (SSP) and di-ammonium phosphate (DAP)) applied at 90 kg ha−1, and three organic amendments (farmyard manure (FYM), mung bean residue (MBR), and canola residue (CR)) applied at a rate of 10 t ha−1. A control plot (no phosphorus or organic supply) was included. A randomized complete block design (RCBD) with three replications was adopted. Among the fertilization strategies, SSP + FYM outperformed all other P fertilizers combined with legume or nonlegume residues in terms of grains per spike−1 (52), thousand-grain weight (41.6 g), biological yield (9.7 t ha−1), and grain yield (4 t ha−1). Under full irrigation, improved yield, yield components, and profits were obtained compared to the limited irrigation regime. Three clusters were obtained after applying an Agglomerative Hierarchical Clustering (AHC), and Principal Component Analysis (PCA) conferred the positive effects of inorganic P with FYM on the wheat yield and its related parameters. This study indicated that the productivity of wheat under the SSP + FYM fertilization strategy was found to be more economical with respect to the benefit–cost ratio (BCR). The combined application of SSP + FYM was more profitable in terms of a higher BCR (3.25) than other treatments under the full irrigation regime

Experimental Investigation on The Effect of PolyVinyl Alcohol on Cement Fluid Loss in Oil Wells

This study presents the effect of PolyVinyl Alcohol Cement Fluid Loss Additive (PVA FLAC) on the API Filtration property of a cement slurry. In Cementing operations of oil wells, different type of fluid loss agents and chemicals are used based on the condition of the wells. PVA FLAC recently introduced in Kurdistan oil industry as loss agent additive to reduce the API Filtration Rate and fluid loss of the cement slurry. Four cementing formulations with different concentration of PVA FLAC additive prepared and their API filtration rates are measured. The results showed that when adding 1.94 gm, 2.91 gm and 3.88 gm of PVA FLAC additive to the cement slurry, the API Filtration Rate reduced by %17, %29 and %42, respectively. The new concentration significantly reduced the rate of filtration when compared to using only 0.97 gm of PVA FLAC additive

Incidence and Impact of Baseline Electrolyte Abnormalities in Patients Admitted with Chemotherapy Induced Febrile Neutropenia

BACKGROUND: Febrile neutropenia (FN) and myelosupression remain a challenging oncologic medical emergency and dose limiting toxicity associated with chemotherapy for cancers. Various factors are known to affect the outcomes for patients diagnosed with FN. Electrolyte abnormalities have commonly been observed, but the real incidence and their impact has been only scarcely studied in literature

Integrated use of phosphorus fertilizer and farmyard manure improves wheat productivity by improving soil quality and P availability in calcareous soil under subhumid conditions

IntroductionLow soil fertility and high fertilizer costs are constraints to wheat production, which may be resolved with integrating fertilizer phosphorus (P) and farm-yard manure (FYM). Study objectives were to evaluate P source impacts on soil, P efficiency, and wheat growth in a calcareous soil.MethodsTreatments included P fertilizer (0, 17, 26, or 39 kg P ha-1) and/or FYM (0 or 10 T ha-1) in a: 1) incubation experiment and 2) wheat (Triticum aestivum spp.) field experiment.Results and DiscussionSoil organic matter increased (30-72%) linearly for both fertilizer and FYM, whereas pH decreased (0.1-0.3 units) with fertilizer only. Addition of fertilizer and FYM increased plant available P (AB-DTPA extractable soil P) an average of 0.5 mg P kg-1 soil week-1 with incubation. The initial increase was 1-9 mg P kg-1, with further increase after 84 d of ~3-17 mg P kg-1. There was also a significant increase of available P in the soil supporting plants in the field study, although the magnitude of the increase was only 2 mg kg-1 at most for the highest fertilizer rate + FYM. Grain (66 to 119%) and straw (25-65%) yield increased significantly, peaking at 26 kg P ha-1 + FYM. The P Absorption Efficiency (PAE), P Balance (PB), and P Uptake (PU) increased linearly with P rate, with the highest levels at the highest P rate. The P Use Efficiency (PUE) was highest at the lowest rates of P, with general decreases with increasing P, although not consistently. Principal component analysis revealed that 94.34 % of the total variance was accounted for with PC1 (84.04 %) and PC2 (10.33 %), with grain straw yield significantly correlated to SOM, PU, and PAE. Regression analysis showed highly significant correlation of PB with P-input (R2= 0.99), plant available P (R2= 0.85), and PU (R2= 0.80). The combination of FYM at the rate of 10 T ha-1 and fertilizer P at 26 kg P ha-1 was found as the optimum dose that significantly increased yield. It is concluded that FYM concoction with fertilizer-P not only improved SOM and residual soil P, but also enhanced wheat yields with reasonable P efficiency

TAT-peptide conjugated repurposing drug against SARS-CoV-2 main protease (3CLpro): potential therapeutic intervention to combat COVID-19

The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that originated in Chinese city of Wuhan has caused around 906,092 deaths and 28,040,853 confirmed cases worldwide (WHO, 11 September, 2020). In a life-threatening situation, where there is no specific and licensed anti-COVID-19 vaccine or medicine available; the repurposed drug might act as a silver bullet. Currently, more than 211 vaccines, 80 antibodies, 31 antiviral drugs, 35 cell-based, 6 RNA-based and 131 other drugs are in clinical trials. It is therefore utter need of the hour to develop an effective drug that can be used for the treatment of COVID-19 before a vaccine can be developed. One of the best-characterized and attractive drug targets among coronaviruses is the main protease (3CL^{pro}). Therefore, the current study focuses on the molecular docking analysis of TAT-peptide^{47–57} (GRKKRRQRRRP)-conjugated repurposed drugs (i.e., lopinavir, ritonavir, favipiravir, and hydroxychloroquine) with SARS-CoV-2 main protease (3CL^{pro} to discover potential efficacy of TAT-peptide (TP) - conjugated repurposing drugs against SARS-CoV-2. The molecular docking results validated that TP-conjugated ritonavir, lopinavir, favipiravir, and hydroxychloroquine have superior and significantly enhanced interactions with the target SARS-CoV-2 main protease. In-silico approach employed in this study suggests that the combination of the drug with TP is an excelling alternative to develop a novel drug for the treatment of SARS-CoV-2 infected patients. The development of TP based delivery of repurposing drugs might be an excellent approach to enhance the efficacy of the existing drugs for the treatment of COVID-19. The predictions from the results obtained provide invaluable information that can be utilized for the choice of candidate drugs for in vitro, in vivo and clinical trials. The outcome from this work prove crucial for exploring and developing novel cost-effective and biocompatible TP conjugated anti-SARS-CoV-2 therapeutic agents in immediate future

Factors influencing in-hospital length of stay and mortality in cancer patients suffering from febrile neutropenia

Introduction: Febrile neutropenia (FN) is a major complication of chemotherapy, costly in terms of morbidity, mortality and associated financial expenditure. The present study was conducted with the goal of highlighting FN as a serious problem in Pakistan, with the longer term objective of improved cancer survival, reduction in length of stay (LOS) in hospital, morbidity, mortality and costs in our existing developing country scenario. Methods: A cross-sectional descriptive study was conducted on Patients, \u3e or =18 years, admitted with FN as a consequence of chemotherapy at a referral hospital in Karachi from 1st September 2006 to 30th April 2007. Results: A total of 80 Patients [43 (53.8%) males and 37 (46.2%) females] were selected. The mean age was 47.4 (SD +/-16.6, range 18-79) years. Sixty eight Patients (86%) were \u3c or = 65 years, 50% were \u3c or = 50 years. Overall, inhospital mortality was 11%, 4% for Patients on granulocyte colony stimulating factor (G-CSF) prophylaxis as against 20% for those without. The cause of death was either pneumonia or septic shock. Mean LOS was 7.53 (SD +/-3.8, range 2-17) days. Hematological malignancies, older age, severity of dehydration, pneumonia and culture positivity were significantly associated with LOS and death. Those above 50 years of age were 1.5 times as likely to be hospitalized longer and \u3e three times as likely to die. Bacteremia conferred a 5-fold and pneumonia an 8-fold increase in the risk of death. Conclusion: The results of this study indicate that age, vital instability, dehydration, high creatinine, culture positivity and hematological malignancies are high risk factors in chemotherapy induced FN. Identification of FN risk factors with poor outcomes may help in devising protocols for modified dosage or including GCFs initially. This may help reduce the cost of cancer care as well as mortality and morbidity. Prospective studies of FN in multiple centers in Pakistan may be beneficial in evaluating these risk factors further

Métastases leptoméningées d’un adénocarcinome gastrique simulant un hématome extradural subaigu

Les métastases intracrâniennes sont de loin les tumeurs malignes les plus fréquentes du système nerveux central, puisqu’elles représentent un peu plus du quart des ces néoplasmes de l’adulte. Des formes simulant des méningiomes ou un hématome sous dural ont été décrites. Les métastases leptoméningées dans le cadre du cancer gastrique sont extrêmement rares. Observation: Un patient de 69 ans, ayant comme antécédent un adénocarcinome gastrique opéré il y a un an ; qui a présenté des céphalées rebelles aux antalgiques usuels et des troubles du comportement, faiblesse progressive de l’hémi-corps droit. L’examen neurologique trouve une hémiparésie spastique droite cotée à 4 /5 à prédominance brachiale, et un syndrome frontal. Un scanner et l’IRM cérébrale objectivaient une lésion extra-durale frontale gauche spontanément hyperdense à la TDM et en hyper signal T1 avec oedème à l’IRM. Une craniectomie frontale gauche effectuée, en urgence, a permis de découvrir une tumeur leptoméningée. L’examen histologique qui a confirmé l’origine gastrique. Discussion: Les métastases cérébrales se produisent dans environ 20% des patients atteints de tumeurs malignes. Le risque est plus élevé dans les cancers bronchiques et cancer du sein. Les métastases cérébrales des cancers gastriques sont extrêmement rares et diagnostiquées chez moins de 1% des patients touchés. La réponse au traitement est médiocre. Conclusion: Bien que son incidence ne soit que de 1%, mais il est particulièrement important d’en faire le diagnostic car, un traitement adapté spécifique combinant plusieurs armes thérapeutiques peut permettre une survie prolongée avec une bonne qualité de vie

Modeling the Bulk and Nanometric Dielectric Functions of Au and Ag

In this work, we model the dielectric functions of gold (Au) and silver (Ag) which are typically used in photonics and plasmonics. The modeling has been performed on Au and Ag in bulk and in nanometric states. The dielectric function is presented as a complex number with a real part and an imaginary part. First, we will model the experimental measurements of the dielectric constant as a function of the pulsation ω by appropriate mathematical functions in an explicit way. In the second part we will highlight the contributions to the dielectric constant value due to intraband and interband electronic transitions. In the last part of this work we model the dielectric constant of these metals in the nanometric state using several complex theoretical models such as the Drude Lorentz theory, the Drude two-point critical model, and the Drude three-point critical model. We shall comment on which model fits the experimental dielectric function best over a range of pulsation