Investigation of the antioxidant activity and quantification of the amount of heavy metals and some vitamins in carrot

Background: Carrot is a root vegetable from the Umbelliferae family. It is a biennial plant grown for their edible root. Carrots are a good source of carbohydrates and minerals like Calcium, Phosphorus, Iron and Magnesium and may contain toxic amounts of metals as a result of run off effects. It is also rich in carotene, niacin, riboflavin, thiamine and vitamin C.Objectives: To determine the amounts of toxic heavy metals, quantify the amounts of vitamins A and E and investigate the antioxidant activities of Carrot.Method: The research investigated the antioxidant properties of carrot on the basis of the radical scavenging activity on DPPH (1,1-diphenyl-2-picryl hydrazyl), heavy metal analysis were carried out using Flame Atomic Absorption Spectrophotometer while analysis of vitamins was done using HPLC (High Performance Liquid Chromatography).Result: The carrot sample analyzed contained considerable amount of some toxic metals of interest (Cr 0.024, 0.105ppm; Cu 15.76, 30.95ppm; Fe 66.94, 103.95ppm; Zn 16.57, 44.22ppm; Pb 0.018, 0.021ppm) in the leaves and root respectively. The samples also contain a very good amount of the vitamin A (12.863, 44.977ppm) and Vitamin E (0.087, 0.22ppm) in leaves and root respectively. It also showed some antioxidant activity and test positive for most phytochemicals.Conclusion: The actual concentrations of the respective heavy metals found in two parts of the D. carota samples were within the threshold limit but there was slight variation in the amount present in the root D. carota as compared to its leaf. The root had more concentrations of the metals and this could be due to the fact that the root is more exposed to these metals during plant uptake.Keywords: Heavy metals, Vitamin, DPPH, Antioxidant, Daucus carot

Adequacy of Bristol stool form scale in the assessment of stools by mothers of healthy infants in Ibadan, Nigeria

Background: An important complaint of mothers is the stool form of their infants. In the evaluation of stool forms, healthcare workers usually depend on mothers’ reports which are subjective and influenced by interpretation bias by health care personnel. There is therefore the need for an objective method of describing stool forms. In the present study, we evaluated the utility of the 7-point Bristol Stool Form Scale (BSFS) when used by mothers of healthy Nigerian infants. Materials and Methods: The mothers of 122 healthy infants delivered at term, with infants less than six months of age attending the Infant Welfare Clinics of two health facilities in Ibadan, South West, Nigeria for routine immunisation were enrolled. Mothers were requested to identify their child’s most recent stool form using the 7-point BSFS. Results: The mean ± SD age of the 122 study infants was 80 ±41.6 days (range 4-180 days). Eighty-eight (72.1%) infants were exclusively breastfed and 34 (27.9%) were on mixed feeding. Almost all (120; 98.4%) mothers felt that the BSFS adequately described their infants’ stools. The most commonly reported stool types were T ype 6 (56.7%) and T ype 7 (33.3%). There was no association between reported stool consistency and infant feeding type, level of maternal education and number of previous babies nursed. Conclusion: This study indicated that BSFS may be a reliable tool for mothers to describe stool consistency in healthy Nigerian infants

Phytochemical screening, antioxidant activity, antimicrobial activity and heavy metal quantification of Chrysophyllum albidum fruit extract (sapotaceae)

Background: Chrysophyllumalbidum (Sapotaceae) is a forest fruit tree described by the Scottish botanist George Don. It is commonly found throughout tropical Africa with the common name “white star apple”. Chrysophyllumalbidumis popularly called Agbalumo in the Southwestern region of Nigeria andclosely related to the African star apple (Chrysophyllumafricanum).Objectives: This study aims to determine the phytochemical constituents of the Chrysophyllumalbidum fruits, quantify its heavy metals composition and also determine the antioxidant and antimicrobial activity of the fruit.Material and Methods: Phytochemical screening of the fruit extract was carried out using standards methods while the antioxidant activity was done using DPPH (2, 2-Diphenyl-1-picrylhydrazyl). Atomic absorption spectrophotometer was used to detect and quantify the level of some toxic metal (Cadmium, Lead, Iron, Copper, Chromium, Zinc) contamination of the fruit. Antibacterial assay was carried out using disc diffusion method with measured zones of inhibition.Results: Results obtained reveal the presence of flavonoids, indicative of its antioxidant potentials, tannins and saponin. Heavy metals were also found to exist in varying amount in the fruit with the conspicuous absence of Cadmium. The antimicrobial assay showed increasing activities with increasing concentrations.Conclusion: The fruit of C. albidum, thus possesses antibacterial and antioxidant activities and are so beneficial for consumption. However stringent environmental control needs to be put in place to minimise the level of soil contamination with heavy metals through various human explorative activities.Keywords: Heavy metal, Antioxidant, Antimicrobial, Chrysophyllumalbidu

Assessment of some heavy metals concentrations in soil and groundwater around refuse dumpsite in Ibadan Metropolis, Nigeria

This study reports the determination of some heavy metals’ concentration in soil and groundwater around refuse dumpsites in five locations in Ibadan Metropolis, Nigeria. Five samples were collected from each soil and ground water locations and analysed for heavy metals(Cd, Co, Pb, Cr, Zn, Mn and Ni)using Atomic Absorption Spectrophotometry. The soil samples were obtained in triplicates at 25cm depth intervals whereas the water samples were obtained from hand dug wells less than 10 m deep. The range of the values of heavy metal concentrations obtained in water is Co (0.01-0.04), Pb (0.01-0.02), Cr (0.02-0.05), Zn (0.40-0.70), Mn (0.02-0.05) and Ni (0.02-0.05), but Cadmium was not detected. The concentrations of Pb and Ni in ground water obtained from Arapaja, Aleshinloye and Alafara were higher than the World Health Organization (WHO) and Standard Organisation of Nigeria (SON) limits. The results obtained showed that the concentrations of Cd, Co, Pb, Ni and Cr in soil exceeded the WHO and SON permissible limits. However, Zn and Mn were below the limit. Keywords: Heavy metals, Dumpsite, Groundwater, Soil samples, Concentrations

Phenotypic identification of soil bacterial and fungal communities inhabiting an archaeological monument at Augustine University, Ilara Epe, southwest Nigeria

Background: The Sungbo Eredo Monument is an ancient public work with a system of defensive walls and ditches located in Eredo Local Council Development Area of Epe, Lagos State, southwest Nigeria. A huge section of the monument cuts through the Augustine University campus, forming two-sided vertical walls with a deep ridge in-between. The objective of this investigative study is to determine the microbial profile of soil samples from the monument in the University campus. Methodology: Soil samples were collected from the topsoil at a depth of 7.5cm from four randomly selected points along the edge of the monument. The samples were transported to the microbiology laboratory of the Department of Biological Sciences of Augustine University for analysis. Samples were cultured on Nutrient agar (NA) and incubated aerobically for 24-48 hours for bacteria isolation and on Sabouraud’s Dextrose agar (SDA) for 72 hours for fungi isolation. Bacterial colonies on NA were preliminarily identified to genus level by Gram reaction and conventional biochemical test scheme for Gram-positive (catalase, coagulase, starch hydrolysis) and Gram-negative isolates (oxidase, urease test, indole, methyl red, Voges Proskauer and sugar fermentation tests). Fungi colonies on SDA were identified using conventional macroscopic and microscopic characteristics. Antibiotic susceptibility test of the bacterial isolates to selected antibiotics was done using the Kirby Bauer disc diffusion method. Results: A total of twenty-three bacterial isolates in four genera; Bacillus, Staphylococcus, Cellobiococcus and Micrococcus and nine fungal isolates in three genera; Saccharomyces, Aspergillus and Botrytis were identified from the cultures. The bacterial isolates were sensitive (>50% sensitivity) to only gentamicin and ofloxacin, with 65.2% and 78.3% sensitivity rates respectively, while they were largely resistant to all other antibiotics such as ceftriaxone, erythromycin, cefuroxime, cloxacillin, ceftazidime and augmentin, with resistance rates of 65.2%, 65.2%, 73.9%, 82.6%, 86.9%, 91.3% respectively. Conclusion: The results of this investigative study revealed the presence of antibiotic-resistant bacteria (mainly Gram-positive) and fungi on the archaeological monument of Augustine University, adding to the existing data on microbial spectrum of archaeological monuments that could be useful for unraveling human cultural habits and microbe-related human diseases. However, further studies on molecular identification of these microbial spectrum will be required to ascertain their genetic relatedness and ancestral phylogeny, which will be useful for archaeologists in their study of the Sungbo-Eredo ancestral monument.   French title: Identification phénotypique des communautés bactériennes et fongiques du sol habitant un monument archéologique à l'Université Augustine, Ilara Epe, sud-ouest du Nigeria Contexte: Le monument Sungbo Eredo est un ancien ouvrage public doté d'un système de murs défensifs et de fossés situé dans la zone de développement du conseil local d'Eredo à Epe, dans l'État de Lagos, au sud-ouest du Nigéria. Une énorme section du monument traverse le campus de l'Université Augustine, formant des murs verticaux à deux côtés avec une crête profonde entre les deux. L'objectif de cette étude d'investigation est de déterminer le profil microbien d'échantillons de sol provenant du monument du campus universitaire. Méthodologie: Des échantillons de sol ont été prélevés dans la couche arable à une profondeur de 7,5 cm à partir de quatre points choisis au hasard le long du bord du monument. Les échantillons ont été transportés au laboratoire de microbiologie du Département des sciences biologiques de l'Université Augustine pour analyse. Les échantillons ont été cultivés sur gélose nutritive (NA) et incubés en aérobie pendant 24 à 48 heures pour l'isolement des bactéries et sur gélose au dextrose de Sabouraud's(SDA) pendant 72 heures pour l'isolement des champignons. Les colonies bactériennes sur NA ont été préalablement identifiées au niveau du genre par réaction de Gram et schéma de test biochimique conventionnel pour les isolats Gram-positif (catalase, coagulase, hydrolyse de l'amidon) et Gram-négatif (oxydase, test à l'uréase, indole, rouge de méthyle, Voges Proskauer et sucre essais de fermentation). Les colonies de champignons sur SDA ont été identifiées en utilisant des caractéristiques macroscopiques et microscopiques conventionnelles. Le test de sensibilité aux antibiotiques des isolats bactériens à des antibiotiques sélectionnés a été effectué en utilisant la méthode de diffusion sur disque de Kirby Bauer. Résultats: Un total de vingt-trois isolats bactériens dans quatre genres; Bacillus, Staphylococcus, Cellobiococcus et Micrococcus et neuf isolats fongiques de trois genres; Saccharomyces, Aspergillus et Botrytis ont été identifiés à partir des cultures. Les isolats bactériens étaient sensibles (sensibilité >50%) uniquement à la gentamicine et à l'ofloxacine, avec des taux de sensibilité de 65,2 % et 78,3 % respectivement, alors qu'ils étaient largement résistants à tous les autres antibiotiques comme la ceftriaxone, l'érythromycine, la céfuroxime, la cloxacilline, la ceftazidime et l'augmentine avec des taux de résistance de 65,2%, 65,2%, 73,9%, 82,6%, 86,9%, 91,3% respectivement. Conclusion: Les résultats de cette étude d'investigation ont révélé la présence de bactéries résistantes aux antibiotiques (principalement à Gram positif) et de champignons sur le monument archéologique de l'Université Augustine, ajoutant aux données existantes sur le spectre microbien des monuments archéologiques qui pourraient être utiles pour démêler l'homme. les habitudes culturelles et les maladies humaines liées aux microbes. Cependant, d'autres études sur l'identification moléculaire de ces spectres microbiens seront nécessaires pour déterminer leur parenté génétique et leur phylogénie ancestrale, ce qui sera utile aux archéologues dans leur étude du monument ancestral Sungbo-Eredo

Paediatric ocular and adnexa malformation in Birnin Kebbi, northwestern Nigeria

Objectives: Congenital eye and adnexa malformations although rare, are however a major challenge to health professionals in terms of diagnosis. There are also emotional, financial and stigmatization challenges experienced by the patients and their relatives. Appropriate management of these patients requires multidisciplinary approach. The study is to determine the pattern and presentation; and also create awareness among health workers and the public on the congenital eye/adnexal malformation.Methods: A 3-year retrospective review of all patients who presented at our hospital on account of congenital eye/adnexal malformations from 1st January 2011 through 31st December 2013 was done. Information on socio-demographics, laterality, type of malformation, presentation and Visual acuity were extracted from patients' folders. The data was analyzed by SPPS version 18.Results: 78 cases of congenital eye/adnexal malformations were recorded during the study period. There were equal numbers of males and females (39 each). Most of the patients were 1year and above (57.7%) at the time of diagnosis. Both eyes were involved in 54 (69%) patients and right eye were 17 (22%). The common congenital eye/adnexal defects identified were congenital ptosis 12 (15.4%), glaucoma 10 (12.8%) and esotropia 9 (11.5%). Majority of the mothers were between 21 and 25years of age (65.4%) as at the time of giving birth to these patients.Conclusion: Congenital ptosis and glaucoma were the commonest types of congenital eye/adnexal defects seen in this environment. These defects were important causes of poor vision and blindness. Parents/caregivers and health professionals awareness on examination, early presentation and treatment is highly required.Keywords: Congenital eye/adnexal malformations, paediatric, parent/caregive

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Fungal strains isolated from fermented maize (ogi) (PW) and sorghum-based brewery wastewaters (BW) and two soil isolates were evaluated for oleaginicity. The fungal isolates from the wastewater that had lipid content of at least 20% of their biomass were identified by both culture methods and internal transcribed spacer (ITS) 1-5.8S-ITS2 ribosomal DNA sequencing. The isolates were identified to be Aspergillus fumigatus (PW8), Aspergillus flavus (PW10), Candida tropicalis (PW16) and Aspergillus tubingensis (PW3), Trichosporon luoberi (BW7), Aspergillus sp. (BW4) and Candida tropicalis (BW1; BW3). FAMEs composition was determined for the four strains with the highest lipid content by acid-catalyzed transesterification and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID). Palmitoleic acid was the dominant fatty acid in M. circinelloides and T. reesei, and the best producers of capric and lauric acids were Aspergillus fumigatus and Aspergillus sp. (BW4), respectively. These fatty acids are beneficial in making cosmetics and pharmaceuticals (antimicrobials and dietary supplements). The analysis of the FAMEs profile in the species indicated low amounts or absence of some key long chain fatty acid (LCFA) constituents of biodiesels. Based on the FAMEs profile of M. circinelloides investigated, this strain could hold promise for use as feedstock for biodiesel with genetic engineering and a tailored lipid production favouring enrichment of LCFA. Keywords: Fungal lipids, wastewater, fatty acid methyl ester, GC-FI

The influence of social networking on food security status of cassava farming households in Nigeria

Open Access Journal; Published online: 4 Jul 2020Food security, at national and household levels, is on the decline because traditional capital (physical, natural, human and financial) has not fully led to its improvement. There is an increasing shift of attention to social capital as an element that explains household food security, but there is a lack of adequately documented information on this. Therefore, this study investigates the effects of social capital on food security, using data collected on a cross-section of 775 cassava farming households from four zones of Nigeria. About 58% of the respondents (cassava farming households) fall under the intermediate category in terms of the benefits received from belonging in social groups. Age and educational level increased the probability to receive benefit from group activities (p < 0.05), while membership density, labor contribution and decision making significantly affected the level of benefit received (p < 0.10). Based on the estimated food security line, 41% of the cassava households were food secure, while 59% were food insecure. Membership density, cash and labor contribution significantly affected food security. Membership density (p < 0.10) and cash contribution (p < 0.05) increased the probability of being food secure. It was recommended that cassava farming households should be encouraged or aided to form cooperatives or farmers’ groups, participate in the activities, and make financial contributions to investments that reduce manual labor-input in the farming activities to enhance household food security

Estimating multidimensional poverty AmongCassava producers in Nigeria: patterns and socioeconomic determinants

Open Access Journal; Published online: 02 Jul 2020The scourge of poverty, including its correlates, has been witnessing an incremental sequence over the years in Nigeria despite the natural endowment of the country. Efforts by various stakeholders to address this problem have not yielded tangible results. Using cross-sectional data collected in 2015 on 775 cassava farmers spread across four geographical zones, this study estimates multidimensional poverty of cassava producers in Nigeria. This is to determine the factors responsible for poverty increase and contribution(s) of these factors to poverty. The study found that about 74% of the respondents were multidimensionally poor. Assets and public/housing utility were the main contributors to aggregate multidimensional poverty index (MPI), while education and health contributed most to povertyreduction. The results also showed major contributing indicators to MPI to be formal employment, school enrolment, years of schooling, frequency of hospital visits, and household assets’ ownership. The South-eastzone of Nigeria had the highest adjusted headcount of poverty among cassava producers. The estimated coefficient of age, farming experience, years of schooling, household size, and access to informal credit were significant determinants of poverty in the study area. In conclusion, the results suggest that although Nigeria is a federation of more than 30 states that continue to rely on nation-wide policy initiatives of the central government, policies on cassava aiming to lift millions of people out of poverty should instead vary according to the peculiar poverty dimensions of each federation unit. We suggest reform in the agriculture sector that will emphasize facilitation and access to incentives (credits, training, extension, cooperate system, etc.) by younger farmers to engage in modern cassava farming, thereby, enhancing the chances of rural cassava growers to move out of poverty

Physical activity level and energy expenditure of clinical physiotherapists and physiotherapy educators in selected tertiary health and educational institutions in South-West Nigeria

Background: Physiotherapists are trained in the use of physical activity for health promotion, and therefore are expected to be physically active themselves. Aim of the study: This study determined the physical activity level and energy expenditure of clinical physiotherapists and physiotherapy educators. Material and methods: The research design is cross-sectional survey. Sixty participants were selected using the consecutive sampling technique. A pedometer (Omron) was used to measure the number of steps taken by the participants, the distance covered, and the overall energy expenditure. The number of steps was used to classify the participants’ physical activity levels. The data obtained were analysed using descriptive statistics and frequency, percentages and chi-square inferential statistics, Pearson product-moment correlation, Spearman’s rank correlation, independent t-tests and Mann-Whitney U tests. The alpha level was set at 0.05. Results: The mean age and BMI of all participants were 36.81±7.86 years and 26.16±4.46kg/m² respectively. The average number of steps taken per day was 8002±3411 and the mean energy expenditure was 248.26±182.92kcal. This study revealed that 21.3% of the participants were sedentary, 27.7% were ‘low active’, 27.7% were moderately active, 10.6% were active and 12.8% were highly active. The Pearson product-moment correlation coefficient showed a significant negative relationship between the number of steps and age (r = -0.292 p= 0.047) and a significant positive relationship between the number of steps and energy expenditure (r = 0.325; p = 0.026), respectively. There was a significant inverse relationship between the number of steps, the energy expenditure and the age of the participants. There was no significant difference in the number of steps per day, PAL and energy expenditure between clinical physiotherapists and physiotherapy educators. Conclusions: Physiotherapists should improve their physical activity levels, as most of them (76.7%) were classified in the low activity level category