Nutritional Value of some Edible Mushrooms from Egbe Farmland, West Yagba Local Government Area, Kogi State, Nigeria

Studies were carried out on four different Species of wild mushrooms: Lentinus subnudus, Chlorophyllum molybditis, Marasmus species and Pleurotus tuberregium. The fungal species were analyzed for their nutritional compositions, biological and Structural characteristics. The fungal species were rich in proteins (amino acids) and carbohydrates. The fibres contained in the different species were also good sources of roughages. Moreover, their low acidity confirms their edibility. This study strongly recommends the commercialization of mushroom production as a means of additional source of protein requirements and income to the people in the developing economy of Nigeri

Characterization of methicillin-resistant Staphylococcus aureus isolates from apparently healthy individuals in Malete, Kwara state, Nigeria

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a common and continuously growing cause of nosocomial and community-acquired staphylococcal infections around the world. Screening for colonization with MRSA is a major aspect of control and limiting the spread of infections cause by this organism. We investigated the carriage of MRSA among apparently healthy individuals in four rural villages: Eleburu, Tapa, Atere and Apo all around semi-urban town-Malete, in Moro Local Government of Kwara State, Nigeria.Methods: Nasal swabs were collected from volunteered individuals and were cultured on mannitol salt agar and blood agar for isolation and identification of Staph aureus using standard microbiological techniques. Susceptibility to cefoxitin disc (30 ag) was used to determine MRSA status of the isolates. Molecular method was used to detect the gene responsible for resistance among MRSA isolates. Antimicrobial susceptibility test to commonly prescribed antibiotics was carried out using discs diffusion method.Results: Total number of individuals carrying Staph aureus in their nostrils was 42 (37.2 %). Antibiotics susceptibility profile of Staph aureus isolates showed 100 % resistance to cefuroxime, cefotaxime, cloxacillin and augmentin, and were 87 %, 81 %, 69 % and 23.8 % and 19 % resistant to tetracycline, ceftriaxone, erythromycin, ofloxacin and gentamicin respectively. A total of 6 (14%) Community –Acquired MRSA (CA-MRSA) isolates were recovered from individuals living in these villages. Molecular method detected muc and mecA genes in all the 6 (100%) CA-MRSA isolates and lukS-lukF was detected in 3 (50%) of the isolates.Conclusion: Detection of CA-MRSA strains among these rural dweller indicates that they are harbouring enhance virulence organism that may manifest a more severe disease condition. The danger associated with high prevalence of multidrug resistant Staph aureus and CAMRSA; and its consequential effects of poor drug administration in Nigeria was discussed. There is need to establish a more strict legislation and enforcement on drug control; and a body that would monitor production and appropriate use of antibiotics in the Nigeria.KEYWORDS: CA-MRSA, Staph aureus, Antibiotics, Rural Villages and Molecular Characterizatio

Itaconic Acid Production from Date Palm (Phoenix Dactylifera L) Using Fungi in Solid State Fermentation

This study evaluates the potentials of Phoenix dactylifera L (Date fruits) as possible alternative raw materials for itaconic acid production using naturally occurring fungi. Date fruit (pulp) was used as a substrate in solid state fermentation for the production of itaconic acid using naturally occurring fungus.The date fruit (pulp) was de-capped from its seed manually with the aid of a knife and dried in an oven at 60 0C and was grounded using an Excella Mixer grinder. The fungus used was naturally isolated by fermentation of substrate (date pulp) and was identified as Aspergillus niger. Proximate analysis was carried out on the substrate using standard methods. Parameters such as substrate concentration, inoculum size and fermentation period were varied using standard methods to determine its effect on itaconic acid production. Assay for itaconic acid production was carried out using standard methods at a wavelength of 385nm. Amount of itaconic acid produced was derived by translation of absorbance values on the itaconic acid curve. The substrate had a high carbohydrate content of 72.29%. The fermentation results showed maximum production of itaconic acid of 20.75±0.25mg/ml using 40g substrate, 15.13±1.13mg/ml using 2 ml inoculums size of spore suspension (2×105 spores/ml) and a maximum yield of 16.88±0.13mg/ml at day 1 of fermentation period. On optimization with 40g substrate and 2 ml inoculums for 3 days a maximum yield was observed at day 2 of fermentation with a maximum yield of 25.00±1.00mg/ml. The highest acidic level throughout the fermentation period was observed to be at pH 4.2. From the study it was concluded that date pulp is a promising substrate and could be utilized by Aspergillus niger for the production of itaconic acid

PRELIMINARY STUDY ON POTENTIAL EDIBLE COATINGS DERIVED FROM CARBOXYL METHYLCELLULOSE AND FUNGI CULTURED METABOLITES ON THE SHELF-LIFE EXTENSION OF SWEET-ORANGE (CITRUS SINENSIS)

This study focused on assessing the potential of formulated edible coatings derived from a metabolite of Trichoderma viride and Penicillium chrysogenum, combined with carboxyl methylcellulose (CMC) on the postharvest storage quality of orange fruits. The cultured metabolite of fungal bioagents combined with CMC, as well as glycerol (plasticizer), inadequate solution ratio based on wettability, was evaluated for microbiological quality and shelf-life extension of sweet orange. Thereafter, ascorbic acid, total soluble solids, pH, percentage weight loss, among other parameters were assessed for 7 weeks. The results of the study revealed that the pH of CMC + Trichoderma viride and CMC + P. chrysogenum coatings had 3.8 ± 0.02 and 3.17 ± 0.06 respectively, while it was reported 2.90 ± 0.04 for uncoated treatment. Also, the ascorbic acid and total soluble solids of the edible coated oranges were higher than the control. In addition, the percentage of weight loss was higher in the uncoated control compared to the potential edible coated oranges. Further, the microbial load count of the potential edible-coated oranges was less compared to the un-coated oranges. In conclusion, this formulated potential edible coating could be further improved upon and optimized for use in prolonging the storage of sweet oranges

Determinants of Depression among University Students in Nigeria

الملخص: تبحث الدراسة محددات الاكتئاب بين طلاب الجامعات في نيجيريا، وقد اُستخدم المنهج الوصفي من خلال تطبيق استبيان في هذه الدراسة. شملت عيّنة الدراسة جميع طلاب الجامعات في نيجيريا، وتكونت عينة الدراسة من 1050 طالبًا جامعيًا. قام الباحثون بإعداد استبانة خاصة للدراسة، باستخدام نمط الاستجابات المغلقة. أظهرت النتائج أن العمر والدخل عوامل مهمة في مستوى الاكتئاب لدى طلاب الجامعات في نيجيريا، بينما نوع الأسرة لم يكن له تأثير، وعليه فقد جاءت التوصيات على أنه يجب على إدارة الجامعة تحسين خدمات الصحة العقلية من خلال زيادة التثقيف الصحي للكشف المبكر والسيطرة على حالات الاكتئاب بين الطلاب.Abstract: The study examined the determinants of depression among university students in Nigeria. Additionally, descriptive research design of survey type was used in this study. The population for this study comprised of all Nigeria undergraduate students. The sample for the study was 1,050 undergraduates. The instrument used for this study was a self-constructed, structured close ended questionnaire. Findings revealed that age and income were significant determinants of depression among university students in Nigeria while family type is not. It was therefore recommended that the University management should strengthen mental health services through health education to enable early detection and management of depression among students

Vaginal Candida infection in pregnancy and its implications for fetal well-being

Vaginal Candida infection is one of the most common genital tract infections reported in pregnant women. This study was designed to determine the prevalence of vaginal Candida infection and pattern of Candida species isolates in the genital tract of pregnant women during antenatal period and in early labour; and the associated fetal outcome. The study was conducted at the antenatal clinic and labor ward of Olabisi Onabanjo University Teaching Hospital Sagamu, Ogun State, Nigeria. High vaginal swabs were collected from 408 pregnant women at the antenatal clinic and repeated in early labour. The samples were processed to isolate Candida species. Data were analysed using Statistical Package for Social Science (SPSS) windows version 21.0 (IBM Corp., Armonk, NY, USA). Prevalence of Candida infection was significantly higher in early labour (46%) than during antenatal period (38%) (P=0.02). Candida albicans was the predominant isolate, followed by Candida glabrata and Candida tropicalis. Candida infection was associated with increased likelihood of low birth weight babies (AOR 2.8, CI: 1.1-6.8; P= 0.03). However there was no statistically significant effect of Candida infection on the likelihood of preterm delivery (AOR 1.4, CI: 0.7-2.6; P= 0.35). Routine screening and prompt treatment of women at risk of delivering low birth weight babies is advocated. Keywords: Candida; Pregnancy; Preterm birth; Prevalence   L'infection vaginale à Candida est l'une des infections des voies génitales les plus courantes signalées chez les femmes enceintes. Cette étude a été conçue pour déterminer la prévalence de l'infection vaginale à Candida et le profil des isolats d'espèces de Candida dans le tractus génital des femmes enceintes pendant la période prénatale et au début du travail; et l'issue foetale associée. L'étude a été menée à la clinique prénatale et au service d'accouchement de l'hôpital universitaire Olabisi Onabanjo de Sagamu, dans l'État d'Ogun, au Nigéria. Des écouvillons vaginaux élevés ont été prélevés sur 408 femmes enceintes à la clinique prénatale et répétés au début du travail. Les échantillons ont été traités pour isoler les espèces de Candida. Les données ont été analysées à l'aide de la version 21.0 de Windows Package for Social Science (SPSS) (IBM Corp., Armonk, NY, USA). La prévalence de l'infection à Candida était significativement plus élevée au début du travail (46%) qu'au cours de la période prénatale (38%) (P = 0,02). Candida albicans était l'isolat prédominant, suivi de Candida glabrata et Candida tropicalis. L'infection à Candida était associée à une probabilité accrue de bébés de faible poids à la naissance (AOR 2,8, IC: 1,1-6,8; P = 0,03). Cependant, il n'y avait aucun effet statistiquement significatif de l'infection à Candida sur la probabilité d'accouchement prématuré (AOR 1,4, IC: 0,7-2,6; P = 0,35). Le dépistage systématique et le traitement rapide des femmes à risque d'accoucher de bébés de faible poids à la naissance sont recommandés. Mots-clés: Candidose; Grossesse; Naissance prématurée; Prévalenc

Phenolic Extract from Moringa oleifera

This study was designed to determine the antioxidant properties and inhibitory effects of extract from Moringa oleifera leaves on angiotensin-I-converting enzyme (ACE) and arginase activities in vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavenged NO∗, OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50 values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50 values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗, NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by which M. oleifera leaves could be used in the treatment and/or management of erectile dysfunction

Molecular Identification and Phylogenetic Analysis of Fungi Contaminants Associated with In Vitro Cultured Banana Based on ITS Region Sequence

This study characterized, identified and conducted phylogenetic analysis on fungi contaminants in vitro bananas based on the sequence of inter-space (ITS) regions. Genomic DNA was extracted from the pure culture of fungi contaminants, amplified and sequenced using ITS1 and ITS4 markers. Analysis of the sequences using MEGA 7 Software at higher similarity sequence identified five Aspergillus spp., three Penicillium spp., one each of Fusarium, Trichoderma and Cladosporium as the contaminants. The genetic distance between the fungi species was 0.205, which suggests a homogeneous substitution between the sequences, and thiamine was the most stable. The fungi clustered in three major groups at 0.10 genetic distance, subdivided into five clusters. A cluster and sub-cluster consisting of five Aspergillus strains; a major cluster of three Penicillium strains; a cluster comprising of Fusarium chlamydosporum and Trichoderma viride; and a sole fungi Cladosporium tenuissimum. The Aspergillus group were phylogenetically related to A. flavus and A. parvissclerotigenus, the identified Penicillium spp. were closely related to Penicillium citrinum while the detected Cladosporium aligned with Cladosporium tenuissium and Phoma multirostrata. The information provided by this study could be utilized to develop a specific and compelling sterilization protocol to minimize the rate of contamination during in vitro culture procedures