Prebiotics may alter bile salt hydrolase activity: Possible implications for cholesterol metabolism

Probiotics secrete bile salt hydrolase (BSH) which catalyses the deconjugation and excretion of bile salts in the GI tract altering cholesterol metabolism in the liver. Many probiotic preparations include prebiotics to promote probiotic growth but little is understood about how prebiotics affect BSH activity. In this study the ability of probiotic Lactobacilli species to deconjugate bile salts in the presence of various prebiotics was determined by measuring cholic acid release. The kinetic properties of BSH was assessed to determine the impact the prebiotics on bile salt deconjugation. When L. acidophilus NCTC 1723 was incubated with inulin (1%) there was a significant (p < 0.01) increase in cholic acid release by 0.16 nmol/min. Lactulose and lactobionic acid at 1% decreased cholic acid release to 0.2 nmol/min and 0.06 nmol/min respectively. In the presence of the pure BSH, inulin and lactulose (0-6%) altered Kₘ and Vₘₐₓ of the enzyme with a Kᵢ of 12% and 10.5% respectively. By contrast, lactobionic acid (2%) increased BSH activity two-fold (p < 0.01). These results confirm that prebiotics are capable of altering BSH activity in vitro. Similar changes in vivo could potentially affect the claimed health benefits of synbiotics particularly where the desired outcome is lowering of serum cholesterol

Hypoxemia predicts death from severe falciparum malaria among children under 5 years of age in Nigeria: The need for pulse oximetry in case management

Background: Oxygen saturation is a good marker for disease severity in emergency care. However, studies have not considered its use in  identifying individuals infected with Plasmodium falciparum at risk of deaths.Objective: To investigate the prevalence and predictive value of  hypoxaemia for deaths in under-5s with severe falciparum malaria infection.Methods: Oxygen saturation was prospectively measured alongside other indicators of disease severity in 369 under-5s admitted to a tertiary  hospital in Nigeria. Participants were children in whom falciparum malaria parasitaemia was confirmed with blood film microscopy in the presence of any of the World Health Organization-defined life-threatening features for malaria.Results: Overall mortality rate was 8.1%. Of the 16 indicators of the disease severity assessed, hypoxaemia (OR=7.54; 95% CI=2.80, 20.29), co-morbidity with pneumonia (OR=19.27; 95% CI=2.87, 29.59), metabolic acidosis (OR=6.21; 95% CI=2.21, 17.47) and hypoglycaemia (OR=19.71; 95% CI=2.61, 25.47) were independent predictors of death. Cerebral malaria, male gender, wasting, hypokalaemia, hyponatriaemia, azotaemia and renal impairment were significantly associated with death in univariate analysis but not logistic regression model.Conclusions: Hypoxaemia predicts deaths in Nigerian children with severe malaria, irrespective of other features. Efforts should always be made to measure oxygen saturation as part of the treatments for severe malaria in children.Keywords: Severe malaria, Under-5 mortality, Oxygen saturation,   Hypoxaemi

Sourcing antimicrobial agents from Globimetula braunii: An in silico molecular docking and dynamic approach

The continued emergence of multi-drug resistance pathogens has been a major setback to lifting the burden of infectious diseases, especially bacterial illnesses. Natural- and/or nature-inspired compounds have so far become a therapeutic backbone on which many novel antibacterial agents are optimized. It is against this backdrop that we used an in silico molecular interaction-based approach to screen five previously identified compounds from Globimetula braunii, for lead inhibitors against bacterial illnesses. The compounds were chromatographed from the leaf ethyl acetate fraction and were characterized by spectroscopic means as 13,27-cycloursane (1), 13,27-cycloursan-3-one (2), methyl-3,5-dihydroxy-4-methoxybenzoate (3), 3-methyl-4-hydroxybenzoate (4), and 2-methoxyphenol (5). Upon their molecular docking at the active pocket of the Staphylococcus aureus gyrase B and the Escherichia coli DNA gyrase, 2 showed the highest binding affinities, with energy scores of -10 and -9.6 Kcal/mol. These were better than the standard antibiotics, Ampicillin (-7.5 and -8.0 Kcal/mol), and Ciprofloxacin (-6.9, -8.4 Kcal/mol). Further evaluation of the most promising compound 2 by molecular dynamics simulation showed the mean RMSD values of the 13,27-cycloursan-3-one - E. coli DNA gyrase protein complexes (complex 1) and 13,27-cycloursan-3-one - S. aureus gyrase B protein (complex 2) to be 0.7 and 0.9 Ǻ respectively, attaining stability at 102 and 108 ns. In contrast, complexes 1 and 2’s RMSF analysis revealed the fewest fluctuations and was generally stable over the course of the 120 ns. In conclusion, 13,27-cycloursan-3-one is unquestionably the most promising inhibitory candidate against the bacterial growth protein DNA gyrase, hence, it can be considered as a druggable substance against bacterial disease

Epidemiology and clinical profile of vitiligo in Ibadan, Nigeria

Background: Vitiligo patients are seen routinely in the Dermatology Outpatient Clinic of the University College Hospital Ibadan, Nigeria. However, the epidemiologic and clinical profile of these patients is not documented. The aim of this study was to document the clinical (age of onset, location, severity, class of vitiligo) and sociodemographic characteristics of these patients.Methods: This is a retrospective descriptive study of patients treated for vitiligo from January 2005 to December 2009 at the University College Hospital (UCH) Ibadan dermatology outpatient clinic. Patient records were retrieved and a study proforma was used to assess patient’s information. Data was analyzed using SPSS version 16.Results: Over the study period, 130 vitiligo patients seen but only 111 case notes were retrieved. The mean age at onset in children was 7.8±4.0 and 34.6±17.1 in adults, M:F was 1:1. The commonest type of vitiligo was acrofacial, area of onset was the face/scalp in 59.2%, re-pigmentation was reported in 66%, active lesions in 59.7%, severity of vitiligo was <9% in 90%, Vitiligo was in visible (exposed) parts of the body 84.7% and asymptomatic in 92.7%.Conclusions: The clinical profile of vitiligo in Ibadan, Nigeria is similar to that observed in other places with only females having a genital onset and acrofacial vitiligo being the dominant type seen. Also, treatment modality varies between children and adults

Pollution Studies on Ground Water Contamination: Water Quality of Abeokuta, Ogun State, South West Nigeria

Changes in water levels in wells reflect changes in recharge to, and discharge from an aquifer. Generally groundwater is rain water or water from surface water bodies, like rivers, lakes or streams that permeate into the soil and bedrock and is stored underground in the pores spaces between soil particles. Groundwater pollution occurs when hazardous substances come into contact and dissolve in the water in the soil or on the surface. Groundwater is generally less susceptible to contamination and pollution when compared to surface water, naturally impurities in rainwater or surface water which replenishes groundwater systems, get removed while infiltrating through soil strata. In Nigeria, groundwater is used intensively for irrigation and domestic purposes, a variety of land and water-based human activities are causing pollution of this precious resource. Its over-exploitation is causing aquifer contamination in certain instances, while in certain others its unscientific development with insufficient knowledge of groundwater flow dynamic and geo-hydro-chemical processes has led to its mineralization. Adequate supply of safe and portable fresh water is an inevitable factor for socio economic development.  Although the recent global attention focuses on how the current and foreseeable water crisis and associated consequences would be addressed, quite a lot of factors such as  low level of education, insufficient budgetary funding, inefficient government policies,  drought are increasingly contributing to the pollution of domestic water in Nigeria. Keywords: Nigeria, Groundwater, Pollution, soil strata, Qualit

Community Based Study of the Influence of Social class on the Prevalence and Clinical Profile of Adolescent Facial Acne Vulgaris

Background: Facial acne vulgaris is the most common disease of the skin in adolescents. In Nigeria, studies of the prevalence of&nbsp; dolescent facial acne vulgaris, lesion type and distribution, and influence of social class on prevalence are few. The aim of this study was to determine the prevalence of adolescent facial acne vulgaris, clinical characteristics, and the influence of social class on this prevalence. Methods: This was a community‑based cross‑sectional study. One thousand and seventy‑nine students from four secondary schools (two private and two public) were assessed. Students were clinically examined for facial acne vulgaris, and acne severity was graded. A structured questionnaire for sociodemographic variables was administered to the students. Data were analyzed using SPSS 16. Results: One thousand and seventy‑nine students were studied; 52% were male, and 47.9% were female. The students were aged 9–20 years with a mean age of 14 ± 2.1 years. The prevalence of facial acne vulgaris was found to be 53.2%. More females (56.7%) had acne than males (50%), the prevalence was found to increase from 28% in the 9–11 years age group to 71.1% in the 18–20 years age group and more prevalent in adolescents from the high social class (60.7%). The severity of acne was mild in 67.2%, moderate in 28.9%, and severe in 4.9%. Conclusion: Prevalence of adolescent facial acne vulgaris increases with age, more prevalent in adolescents from a high social class. Male gender, age, a family history of acne, and previous treatment of acne contribute to the severity of acne. Keywords: Acne vulgaris, adolescent, clinical profile, prevalence, severity, social class&nbsp

Using Marketing Performance Appraisal to Develop Bank Marketing Strategy

The 'strategic posture' of banks depends partly on the competitive environment, partly on its allocation of marketing resources. Strategic posture is determined in the context of the strengths, limitations, and corporate objectives of the bank. This paper presents different marketing strategies for bankers, suggesting that the selection of appropriate strategy should be based on the internal conditions and external forces facing the firm. The selected strategy should then be implemented by a prudent marketing plan. If a bank's marketing strategy is to be effective, due attention should be given to the marketing organizational structure and its department responsibilities. Finally, the study suggests several alternative criteria for evaluating the performance of the selected marketing strategy. Dynamic conditions and strategic objectives should be continually appraised in order that bankers might retain the market advantag