Power calculations for isentropic compressions of cryogenic nitrogen

A theoretical analysis was made of the power required for isentropic compressions of cryogenic nitrogen in order to determine the extent to which the drive power for cryogenic tunnels might be affected by real-gas effects. The analysis covers temperatures from 80 to 310 K, pressures from 1.0 to 8.8 atm, and fan pressure ratios from 1.025 to 1.200. The power required to compress cryogenic nitrogen was found to be as much as 9.5 percent lower than that required to compress an ideal diatomic gas. Simple corrections to the ideal-gas values were found to give accurate estimates of the real-gas power values

Epigenetics and chromatin remodeling play a role in lung disease

Epigenetics is defined as heritable changes that affect gene expression without altering the DNA sequence. Epigenetic regulation of gene expression is facilitated through different mechanisms such as DNA methylation, histone modifications and RNA-associated silencing by small non-coding RNAs. All these mechanisms are crucial for normal development, differentiation and tissue-specific gene expression. These three systems interact and stabilize one another and can initiate and sustain epigenetic silencing, thus determining heritable changes in gene expression. Histone acetylation regulates diverse cellular functions including inflammatory gene expression, DNA repair and cell proliferation. Transcriptional coactivators possess intrinsic histone acetyltransferase activity and this activity drives inflammatory gene expression. Eleven classical histone deacetylases (HDACs) act to regulate the expression of distinct subsets of inflammatory/immune genes. Thus, loss of HDAC activity or the presence of HDAC inhibitors can further enhance inflammatory gene expression by producing a gene-specific change in HAT activity. For example, HDAC2 expression and activity are reduced in lung macrophages, biopsy specimens, and blood cells from patients with severe asthma and smoking asthmatics, as well as in patients with chronic obstructive pulmonary disease (COPD). This may account, at least in part, for the enhanced inflammation and reduced steroid responsiveness seen in these patients. Other proteins, particularly transcription factors, are also acetylated and are targets for deacetylation by HDACs and sirtuins, a related family of 7 predominantly protein deacetylases. Thus the acetylation/deacetylation status of NF-κB and the glucocorticoid receptor can also affect the overall expression pattern of inflammatory genes and regulate the inflammatory response. Understanding and targeting specific enzymes involved in this process might lead to new therapeutic agents, particularly in situations in which current anti-inflammatory therapies are suboptimal

The impact of working wives\u27 income on household food consumption and expenditures

Economic theory has utilized price and income elasticities of demand in measuring and explaining household food purchase behavior. While these elasticity coefficients have explained quantity change measurements with respect to different income and price levels, a more accurate explanation of the resultant elasticity coefficient may be obtained if causes of variations between households of given income levels can be established. To obtain this added information, a more detailed analysis, with the inclusion of variables in addition to price and income constituents, would be required. An important source of additional income for households in the United States has been that earned by wives in the labor force. The 1960 Census of Population indicated that about 24 percent of all households in metropolitan Knoxville, Tennessee, had both the husband and wife gainfully employed. Results of this study indicate that approximately 26 percent of those households surveyed in Knoxville, Tennessee, had both the husband and wife in the labor force. The effect of the wives\u27 household income upon household food purchases with respect to poundage and expenditure per capita is the primary concern of this study

Dexamethasone and RU24858 induce survival and growth factor receptor bound protein 2, leukotriene B4 receptor 1 and annexin-1 expression in primary human neutrophils

Glucocorticoids are widely used anti-inflammatory medication in diseases like asthma and chronic obstructive pulmonary disease. Glucocorticoids can either activate (transactivation) or inhibit (transrepression) transcription. RU24858 was introduced as a dissociated glucocorticoid and it has been reported to transrepress but not to transactivate. The aim of this study was to compare the effects of RU24858 and dexamethasone in human neutrophils. RU24858 delayed spontaneous neutrophil apoptosis and further enhanced GM-CSF- induced neutrophil survival to a similar extent as dexamethasone. Like dexamethasone RU24858 also reduced CXCL8 and MIP-1α. Unexpectedly however, RU24858 increased the expression of the glucocorticoid-inducible genes BLT-1, Annexin-1 and Grb-2 in neutrophils to a similar level as seen with dexamethasone. We have shown here that dexamethasone and RU24858 both increase Grb-2, BLT1 and Annexin-1 expression and inhibit CXCL8 and MIP-1α production. This suggests that RU24858 was not able to dissociate between transactivation and transrepression in human neutrophils but enhanced neutrophil survival. © the author(s), publisher and licensee Libertas Academica Ltd

Simulation of flight test conditions in the Langley pilot transonic cryogenic tunnel

The theory and advantages of the cryogenic tunnel concept are briefly reviewed. The unique ability to vary temperature independently of pressure and Mach number allows, in addition to large reductions in model loads and tunnel power, the independent determination of Reynolds number, Mach number, and aeroelastic effects on the aerodynamic characteristics of the model. Various combinations of Reynolds number and dynamic pressure are established to represent accurately flight variations of aeroelastic deformation with altitude changes. The consequences of the thermal and caloric imperfections of the test gas under cryogenic conditions were examined and found to be insignificant for operating pressures up to 5 atm. The characteristics of the Langley pilot transonic cryogenic tunnel are described and the results of initial tunnel operation are presented. Tests of a two-dimensional airfoil at a Mach number of 0.85 show identical pressure distributions for a chord Reynolds number of 8,600,000 obtained first at a stagnation pressure of 4.91 atm at a stagnation temperature of 322.0 K and then at a stagnation pressure of 1.19 atm at a stagnation temperature of 116.5 K

Analysis of validation tests of the Langley pilot transonic cryogenic tunnel

A pilot transonic cryogenic pressure tunnel has recently been developed and proof tested at the NASA Langley Research Center. In addition to providing an attractive method for obtaining high Reynolds number results at moderate aerodynamic loadings and tunnel power, this unique tunnel allows the independent determination of the effects of Reynolds number, Mach number, and dynamic pressure (aeroelasticity) on the aerodynamic characteristics of the model under test. The proof of concept experimental and theoretical studies are briefly reviewed. Experimental results obtained on both two- and three-dimensional models have substantiated that cryogenic test conditions can be set accurately and that cryogenic gaseous nitrogen is a valid test medium

The cryogenic wind tunnel concept for high Reynolds number testing

Theoretical considerations indicate that cooling the wind-tunnel test gas to cryogenic temperatures will provide a large increase in Reynolds number with no increase in dynamic pressure while reducing the tunnel drive-power requirements. Studies were made to determine the expected variations of Reynolds number and other parameters over wide ranges of Mach number, pressure, and temperature, with due regard to avoiding liquefaction. Practical operational procedures were developed in a low-speed cryogenic tunnel. Aerodynamic experiments in the facility demonstrated the theoretically predicted variations in Reynolds number and drive power. The continuous-flow-fan-driven tunnel is shown to be particularly well suited to take full advantage of operating at cryogenic temperatures

Exosomes and Exosomal miRNA in Respiratory Diseases

Exosomes are nanosized vesicles released from every cell in the body including those in the respiratory tract and lungs. They are found in most body fluids and contain a number of different biomolecules including proteins, lipids, and both mRNA and noncoding RNAs. Since they can release their contents, particularly miRNAs, to both neighboring and distal cells, they are considered important in cell-cell communication. Recent evidence has shown their possible importance in the pathogenesis of several pulmonary diseases. The differential expression of exosomes and of exosomal miRNAs in disease has driven their promise as biomarkers of disease enabling noninvasive clinical diagnosis in addition to their use as therapeutic tools. In this review, we summarize recent advances in this area as applicable to pulmonary diseases