182 research outputs found

    Editing My Own Drum

    Get PDF
    The author examines her art and poetry practice exploring how the Badlands of Alberta, Canada in particular Áísínai\u27pi (Writing-on-Stone Provincial Park), are the bones of the earth . She looks at the visual and linguistic poetry within them and examines her wording to decolonize her practice and learning/unlearning from the land and the cultures; Blackfoot and Settler, and peoples that inhabit it. She highlights how her process of editing a specific poem “My Own Drum” prompted and echoes an examination of her practice that leads to the writing of a new poem written during this process, revealing some of her findings. The original, edited and new poems show her process and struggles with fear, knowledge, skill, experience, art, poetry, nature and decolonization as a descendant of white settlers on Treaty 7 land. This is her first attempt to de-colonize her practice and she hopes that sharing her journey will inspire others to do so as well

    Changing Adolescents\u27 Food-Related Behavior Via Nutrition Education

    Get PDF
    A nutrition education behavior change strategy for secondary health classes was developed, implemented, and evaluated with 159 adolescents in 6 schools. The experimental design was pretest/posttest, treatment/control group with two treatment groups--one receiving only the behavior change strategy (BC) and one receiving the strategy plus a traditional knowledge-oriented component (BC+). Fundamental concepts of the behavior change strategy included personalization, goal setting, self-management, self-implementation, structured feedback, and structured self-evaluation. Based on a personal nutrient analysis, each adolescent in a treatment group selected improvement of one nutrient as a goal. Effectiveness of the strategy was tested by changes made in nutrient intake related to goal set. Pre- /post-assessments included three-day food records and written questionnaires of food practices and nutrition attitudes and knowledge. A rating instrument was administered to adolescents in treatment groups to assess their perceptions of activities facilitating change. Setting a goal for specific nutrient improvement had the strongest effect on change. No difference between treatment groups was found. Adolescents who set a goal related to calcium, vitamin A, and vitamin C increased intake and those with a sodium-related goal decreased sodium. No positive changes in nutrient intake occurred for the control group. Gender was related to positive change for calcium, vitamin A, folacin, and vitamin C, with males making significant improvements. Nutrition attitudes and knowledge generally were not related to change. Food practice scores, as assessed by the written questionnaire, increased for the BC+ group only. Knowledge scores increased in both treatment groups but not in the control group. No differences in attitudes were seen, either within or among groups. Activities perceived as most helpful in facilitating change included keeping food records, assessing personal nutrient intake, implementing a plan for nutrient improvement, and participating in follow-up classes

    Methods and systems for identifying a particle using dielectrophoresis

    Get PDF
    A system for identifying a particle. The system includes a microfluidic device; a microelectrode array including a plurality of electrodes, the microelectrode array disposed within the microfluidic device; a plurality of particles suspended in a solution and delivered to the microelectrode array using the microfluidic device; a signal generator operatively coupled to the microelectrode array; a particle detector adjacent to the microelectrode array; and a controller in operative communication with the signal generator and the particle detector. The controller is configured to apply an oscillating voltage signal to the microelectrode array between a low frequency and a high frequency at a sweep rate, wherein the sweep rate is no more than a maximum sweep rate, and determine a distribution of the plurality of particles relative to the microelectrode array at a plurality of frequency levels between the low frequency and the high frequency.https://digitalcommons.mtu.edu/patents/1139/thumbnail.jp

    Anaerobic Oxidation of Short-Chain Alkanes in Hydrothermal Sediments: Potential Influences on Sulfur Cycling and Microbial Diversity

    Get PDF
    Short-chain alkanes play a substantial role in carbon and sulfur cycling at hydrocarbon-rich environments globally, yet few studies have examined the metabolism of ethane (C2)(C_2), propane (C3)(C_3), and butane (C4)(C_4) in anoxic sediments in contrast to methane (C1)(C_1). In hydrothermal vent systems, short-chain alkanes are formed over relatively short geological time scales via thermogenic processes and often exist at high concentrations. The sediment-covered hydrothermal vent systems at Middle Valley (MV, Juan de Fuca Ridge) are an ideal site for investigating the anaerobic oxidation of C1–C4C_1–C_4 alkanes, given the elevated temperatures and dissolved hydrocarbon species characteristic of these metalliferous sediments. We examined whether MV microbial communities oxidized C1–C4C_1–C_4 alkanes under mesophilic to thermophilic sulfate-reducing conditions. Here we present data from discrete temperature (25, 55, and 75∘C75^{\circ}C) anaerobic batch reactor incubations of MV sediments supplemented with individual alkanes. Co-registered alkane consumption and sulfate reduction (SR) measurements provide clear evidence for C1–C4C_1–C_4 alkane oxidation linked to SR over time and across temperatures. In these anaerobic batch reactor sediments, 16S ribosomal RNA pyrosequencing revealed that Deltaproteobacteria, particularly a novel sulfate-reducing lineage, were the likely phylotypes mediating the oxidation of C2–C4C_2–C_4 alkanes. Maximum C1–C4C_1–C_4 alkane oxidation rates occurred at 55∘C55^{\circ}C, which reflects the mid-core sediment temperature profile and corroborates previous studies of rate maxima for the anaerobic oxidation of methane (AOM). Of the alkanes investigated, C3C_3 was oxidized at the highest rate over time, then C4C_4, C2C_2, and C1C_1, respectively. The implications of these results are discussed with respect to the potential competition between the anaerobic oxidation of C2–C4C_2–C_4 alkanes with AOM for available oxidants and the influence on the fate of C1C_1 derived from these hydrothermal systems.Molecular and Cellular BiologyOrganismic and Evolutionary Biolog

    Genetic and biochemical differences in populations bred for extremes in maize grain methionine concentration

    Get PDF
    Background: Methionine is an important nutrient in animal feed and several approaches have been developed to increase methionine concentration in maize (Zea mays L.) grain. One approach is through traditional breeding using recurrent selection. Using divergent selection, genetically related populations with extreme differences in grain methionine content were produced. In order to better understand the molecular mechanisms controlling grain methionine content, we examined seed proteins, transcript levels of candidate genes, and genotypes of these populations. Results: Two populations were selected for high or low methionine concentration for eight generations and 40 and 56% differences between the high and low populations in grain methionine concentration were observed. Mean values between the high and low methionine populations differed by greater than 1.5 standard deviations in some cycles of selection. Other amino acids and total protein concentration exhibited much smaller changes. In an effort to understand the molecular mechanisms that contribute to these differences, we compared transcript levels of candidate genes encoding high methionine seed storage proteins involved in sulfur assimilation or methionine biosynthesis. In combination, we also explored the genetic mechanisms at the SNP level through implementation of an association analysis. Significant differences in methionine-rich seed storage protein genes were observed in comparisons of high and low methionine populations, while transcripts of seed storage proteins lacking high levels of methionine were unchanged. Seed storage protein levels were consistent with transcript levels. Two genes involved in sulfur assimilation, Cys2 and CgS1 showed substantial differences in allele frequencies when two selected populations were compared to the starting populations. Major genes identified across cycles of selection by a high-stringency association analysis included dzs18, wx, dzs10, and zp27. Conclusions: We hypothesize that transcriptional changes alter sink strength by altering the levels of methionine-rich seed storage proteins. To meet the altered need for sulfur, a cysteine-rich seed storage protein is altered while sulfur assimilation and methionine biosynthesis throughput is changed by selection for certain alleles of Cys2 and CgS1

    Adherence to the updated guidelines for the prevention of perinatal Group B streptococcal disease

    Get PDF
    In 2010, the Centers for Disease Control and Prevention updated the Guidelines for the Prevention of Perinatal Group B Streptococcal (GBS) Disease. Previous studies of adherence to GBS guidelines have focused on the treatment of carriers of GBS. Our objective was to determine whether there was any difference in adherence to the guidelines for screening and treatment of women who delivered at our institution between the beginning of 2011 and the end of 2011 as the revised guidelines were published in November 2010. Our secondary outcome was to determine whether any differences in adherence occurred between prenatal provider types (OB/Gyn, Certified Nurse Midwives, and Family Practice)

    Oophorectomy Reduces Estradiol Levels and Long-Term Spontaneous Neurovascular Recovery in a Female Rat Model of Focal Ischemic Stroke

    No full text
    Although epidemiological evidence suggests significant sex and gender-based differences in stroke risk and recovery, females have been widely under-represented in preclinical stroke research. The neurovascular sequelae of brain ischemia in females, in particular, are largely uncertain. We set out to address this gap by a multimodal in vivo study of neurovascular recovery from endothelin-1 model of cortical focal-stroke in sham vs. ovariectomized female rats. Three weeks post ischemic insult, sham operated females recapitulated the phenotype previously reported in male rats in this model, of normalized resting perfusion but sustained peri-lesional cerebrovascular hyperreactivity. In contrast, ovariectomized (Ovx) females showed reduced peri-lesional resting blood flow, and elevated cerebrovascular responsivity to hypercapnia in the peri-lesional and contra-lateral cortices. Electrophysiological recordings showed an attenuation of theta to low-gamma phase-amplitude coupling in the peri-lesional tissue of Ovx animals, despite relative preservation of neuronal power. Further, this chronic stage neuronal network dysfunction was inversely correlated with serum estradiol concentration. Our pioneering data demonstrate dramatic differences in spontaneous recovery in the neurovascular unit between Ovx and Sham females in the chronic stage of stroke, underscoring the importance of considering hormonal-dependent aspects of the ischemic sequelae in the development of novel therapeutic approaches and patient recruitment in clinical trials
    • 

    corecore