3D INTERPRETATION AND FUSION OF MULTIDISCIPLINARY DATA FOR HERITAGE SCIENCE: A REVIEW

Activities related to the protection of tangible heritage require extensive multidisciplinary documentation. The various raw data that occur have been oftentimes been processed, visualized and evaluated separately leading to aggregations of unassociated information of varying data types. In the direction of adopting complete approaches towards more effective decision making, the interpretation and fusion of these data in three dimensions, inserting topological information is deemed necessary. The present study addresses the achieved level of three-dimensional interpretation and fusion with geometric models of data originating from different fields, by providing an extensive review of the relevant literature. Additionally, it briefly discusses perspectives on techniques that could potentially be integrated with point clouds or models

An updated comparison on contemporary approaches for digitization of heritage objects

Continuous developments on sensors, data acquisition techniques, algorithms and computational systems have enabled automation, higher processing velocities and increased metric accuracy regarding the modeling of tangible heritage. For applications on heritage artefacts or architectural details, scanning and photogrammetric systems based on structure-from-motion (SfM) approach have prevailed, due to lower costs, fast acquisition and processing, re-producibility of workflows and ability to capture high-resolution texture. This study presents an updated comparison of contemporary digitization approaches to examine in extent required processing stages, compare costs and evaluate produced 3D results according to their metric properties, quality of texture and visual fidelity

γδTCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis.

γδ T cells have been implicated in inflammatory diseases as an important link between the innate and adaptive immune responses, however, their role in inflammatory arthritis remain unclear. To define the contribution of γδ T cells in the pathogenesis of inflammatory arthritis, we performed gene transfer of IL-23 in B10.RIII mice to establish joint inflammation in the presence or absence of γδ T cells. We demonstrated that γδ T cell blockade has a protective effect on arthritis incidence and severity by preventing neutrophil accumulation in the blood, spleen and bone marrow as well as by reducing neutrophil infiltration into the joints. Furthermore, our data demonstrate that absence of γδ T cells was associated with an increase of IL-27 levels produced by neutrophils and dendritic cells, and systemic IL-27 expression also prevents IL-23-induced inflammatory arthritis and limits neutrophil expansion. Collectively our findings reveal an immunomodulatory effect of γδ T cells on neutrophils associated with IL-27 synthesis and secretion and indicate a novel link between IL-27 and the modulation of γδ T cells and neutrophils that can be targeted in the treatment of inflammatory arthritis

AUTOMATIZING DEGRADATION MAPPING OF ANCIENT STELAE BY DUAL-BAND IMAGING AND MACHINE LEARNING-BASED CLASSIFICATION

Degradation patterns are the visible consequence of the impacts of environmental factors and biological agents on stone heritage. Accurately documenting them is a key requisite when studying exposed stone antiquities to interpret weathering causes, identify conservation needs, and plan cleaning interventions. However, a significant gap can be identified in practical automatized procedures for mapping patterns on stone antiquities, such as ancient stelae. This work evaluates a workflow that uses visible and near-infrared imaging, combined with machine learning-based digital image segmentation tools, to classify degradation patterns on marble stelae correctly and cost-effectively. For this work, different classification methods are considered. Results are analyzed using error matrixes and reference degradation maps. The application cases include stelae displayed in the courtyard of the Archaeological Museum of Eretria (Euboea, Greece). The proposed methodology aims at being easily adapted to facilitate the conservators’ work

Immune regulation of bone loss by Th17 cells

A significant macrophage and T-cell infiltrate commonly occurs in inflammatory joint conditions such as rheumatoid arthritis that have significant bone destruction. Cytokines produced by activated macrophages and T cells are implicated in arthritis pathogenesis and are involved in osteoclast-mediated bone resorption. The scope of the present review is to analyze current knowledge and to provide a better understanding of how macrophage-derived factors promote the differentiation of a novel T-helper subset (Th17) that promotes osteoclast formation and activation

FIRST ASSESSMENTS ON HERITAGE SCIENCE ORIENTED IMAGE-BASED MODELING USING LOW-COST MODIFIED AND MOBILE CAMERAS

Three-dimensional modeling of cultural heritage, especially concerning large scale studies, as for example, archaeometry, diagnostics and conservation intervention applications, which usually require high-resolution and multi-spectral analyses, necessitates the use of complicate and often expensive equipment. Recent developments regarding low-cost commercially available spectrally modified digital reflex cameras, smartphones with good quality image sensors, mobile thermal cameras in combination with automated or semi-automated photogrammetric software implementing Structure from Motion (SfM) and Multiview Stereo (MVS) algorithms constitute some cheaper and simpler alternatives. Although, the results of the integration of these types of sensors and techniques are often not evaluated as metric products. The presented research combines the above-mentioned instrumentation and software to implement and evaluate low-cost 3D modeling solutions on heritage science-oriented case studies, but also to perform some first assessments on the resulting models' metric properties, quality of texture and usefulness for further scientific investigations

Three-dimensional thermal mapping from IRT images for rapid architectural heritage NDT

Thermal infrared imaging is fundamental to architectural heritage non-destructive diagnostics. However, thermal sensors’ low spatial resolution allows capturing only very localized phenomena. At the same time, thermal images are commonly collected with independence of geometry, meaning that no measurements can be performed on them. Occasionally, these issues have been solved with various approaches integrating multi-sensor instrumentation, resulting in high costs and computational times. The presented work aims at tackling these problems by proposing a workflow for cost-effective three-dimensional thermographic modeling using a thermal camera and a consumer-grade RGB camera. The discussed approach exploits the RGB spectrum images captured with the optical sensor of the thermal camera and image-based multi-view stereo techniques to reconstruct architectural features’ geometry. The thermal and optical sensors are calibrated employing custom-made low-cost targets. Subsequently, the necessary geometric transformations between undistorted thermal infrared and optical images are calculated to replace them in the photogrammetric scene and map the models with thermal texture. The method’s metric accuracy is evaluated by conducting comparisons with different sensors and the efficiency by assessing how the results can assist the better interpretation of the present thermal phenomena. The conducted application demonstrates the metric and radiometric performance of the proposed approach and the straightforward implementability for thermographic surveys, as well as its usefulness for cost-effective historical building assessments

Interleukin-17A upregulates receptor activator of NF-kappaB on osteoclast precursors.

IntroductionThe interaction between the immune and skeletal systems is evidenced by the bone loss observed in autoimmune diseases such as rheumatoid arthritis. In this paper we describe a new mechanism by which the immune cytokine IL-17A directly affects osteoclastogenesis.MethodsHuman CD14+ cells were isolated from healthy donors, cultured on dentine slices and coverslips and stimulated with IL-17A and/or receptor activator of NF-kappaB ligand (RANKL). Osteoclast differentiation was evaluated by gene expression, flow cytometry, tartrate-resistant acid phosphatase staining, fluorescence and electron microscopy. Physiologic bone remodelling was studied in wild-type (Wt) and IL-17A-/- mice using micro-computer tomography and serum RANKL/osteoprotegerin concentration. Functional osteoclastogenesis assays were performed using bone marrow macrophages isolated from IL-17A-/- and Wt mice.ResultsIL-17A upregulates the receptor activator for NF-kappaB receptor on human osteoclast precursors in vitro, leading to increased sensitivity to RANKL signalling, osteoclast differentiation and bone loss. IL-17A-/- mice have physiological bone homeostasis indistinguishable from Wt mice, and bone marrow macrophages isolated from these mice develop fully functional normal osteoclasts.ConclusionsCollectively our data demonstrate anti-IL-17A treatment as a selective therapeutic target for bone loss associated with autoimmune diseases

Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology.

Autism spectrum disorders (ASDs) are complex and heterogeneous developmental disabilities affecting an ever-increasing number of children worldwide. The diverse manifestations and complex, largely genetic aetiology of ASDs pose a major challenge to the identification of unifying neuropathological features. Here we describe the neurodevelopmental defects in mice that carry deleterious alleles of the Wdfy3 gene, recently recognized as causative in ASDs. Loss of Wdfy3 leads to a regionally enlarged cerebral cortex resembling early brain overgrowth described in many children on the autism spectrum. In addition, affected mouse mutants display migration defects of cortical projection neurons, a recognized cause of epilepsy, which is significantly comorbid with autism. Our analysis of affected mouse mutants defines an important role for Wdfy3 in regulating neural progenitor divisions and neural migration in the developing brain. Furthermore, Wdfy3 is essential for cerebral expansion and functional organization while its loss-of-function results in pathological changes characteristic of ASDs

Myeloid DAP12-associating lectin (MDL)-1 regulates synovial inflammation and bone erosion associated with autoimmune arthritis.

DNAX adaptor protein 12 (DAP12) is a trans-membrane adaptor molecule that transduces activating signals in NK and myeloid cells. Absence of functional Dap12 results in osteoclast defects and bone abnormalities. Because DAP12 has no extracelluar binding domains, it must pair with cell surface receptors for signal transduction. There are at least 15 known DAP12-associating cell surface receptors with distinct temporal and cell type-specific expression patterns. Our aim was to determine which receptors may be important in DAP12-associated bone pathologies. Here, we identify myeloid DAP12-associating lectin (MDL)-1 receptor (also known as CLEC5A) as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Activation of MDL-1 leads to enhanced recruitment of inflammatory macrophages and neutrophils to the joint and promotes bone erosion. Functional blockade of MDL-1 receptor via Mdl1 deletion or treatment with MDL-1-Ig fusion protein reduces the clinical signs of autoimmune joint inflammation. These findings suggest that MDL-1 receptor may be a therapeutic target for treatment of immune-mediated skeletal disorders