2,565 research outputs found

    Breeding Perennial Warm-Season Grasses for the Subtropical Belt in South America

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    Perennial warm-season grasses share several agronomic characteristics, such as marked seasonal growth, cold susceptibility and photoperiod sensitivity. Breeding efforts in South America have been focused on attempting to improved cool-season growth, cold tolerance and also adaptation to the alternation of flooding and drought periods. Warm-season grasses also have in common that most of them are polyploid and some have very low fertility. Apomixis is also a common trait among these species. For polyploid species with limited seed yield, which commonly have stolons or rhizomes, F1 hybrids are created and released as cultivars. Acroceras macrum and Hemarthria altissima will be used as examples. For polyploid species without seed fertility issues, such as Setaria sphacelta, recurrent phenotypic selection (RPS) is used to generate improved populations adapted to these transition zones. For polyploid and apomitic species, such as Paspalum spp. and Brachiara spp., several breeding approaches are now available. The generation of F1 apomictic hybrids is currently used. It has been recently observed that the efficiency of this breeding method can be improved if the genetic distance among parents is considered. There is also new information indicating the great potential of using apomixis-linked molecular markers for the early identification of apomictic hybrids. Population-breeding approaches, such as RPS and selection based on combining ability, can also be used to assess the generation of superior apomictic hybrids. Finally, the challenge of breeding perennial warm-season grasses for the subtropics mainly relates to improving adaptation to extreme conditions (cold winters and warm summers and alternation of flooding and drought), developing specific breeding techniques for polyploid or apomictic species

    Conversation I

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    Documento PDF, 24 páginasGuía de estudio para el curso Conversation I, código 5009, que imparte la Escuela de Ciencias Sociales y Humanidades de la UNED.Universidad Estatal a Distancia de Costa Ric

    A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production

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    IndexaciĂłn: Scopus; Web of Science.Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. The inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant E. coli (ECPi) and P. putida (JBOi) strain such as specific growth rate and biomass yield under standard growth conditions. A different scenario was observed in the recombinant JBOi strain when subjected to PHA-producing conditions, where biomass production was reduced by 25% but the mcl-PHA content was maintained at about 30% of the cell dry weight. Importantly, the genetic construct worked well under PHA-producing conditions (nitrogen-limiting phase), where more than 95% of the cell population presented membrane disruption 16 h post induction, with 75% of the total synthesized biopolymer recovered at the end of the fermentation period. In conclusion, this new lysis system circumvents traditional, costly mechanical and enzymatic cell-disrupting procedures.https://www.nature.com/articles/s41598-017-04741-2.pd

    Contrasting human perceptions of and attitudes towards two threatened small carnivores, Lycalopex fulvipes and Leopardus guigna, in rural communities adjacent to protected areas in Chile

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    Indexación: Scopus.The interaction between humans and small carnivores is a phenomenon especially frequent in rural fringes, as is the case of communities surrounding natural areas. In Chile, two species of threatened carnivores, the Darwin's Fox and the Guigna, have increased their contact with humans due to human-induced changes in their habitat. The objective of this study was to characterize the interactions of these species with humans by assessing human perceptions and attitudes toward them, and to assess livestock and poultry ownership and management practices in local communities to evaluate their possible roles in the phenomenon. We conducted semi-structured interviews in rural communities adjacent to natural protected areas of two different regions in southern Chile. We found that people have a more positive perception of Darwin's Foxes than Guignas, but both species are considered damaging due to poultry attacks. Livestock and poultry management was generally deficient. Improvements in animal management and education programs could lead to a significant decrease in negative interactions. © Sacristan et al. 2018.https://www.threatenedtaxa.org/index.php/JoTT/article/view/4030/442
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