Antibiotic susceptibility pattern of fish pathogens : A new approach of emerging the bacterial resistance through biofilm formation in in-vitro condition

Background: The ability of many bacteria to adhere on the host surfaces and forming biofilms has major implications in a wide variety of industries including the food industry, where biofilms may create a persistent source of contamination. In the same environmental condition, the multiple bacterial species can closely interact with each other and may easily enhance their drug resistance capability, which finally increases the multidrug resistant (MDR) attribute of the species. Objective: The present study examined whether the mixed-species biofilm possesses any impact on the enhancement of the antibiotic resistance of the planktonic or single-cell bacterial isolates present in the fish samples. Methods: In this regard, Cyprinus rubrofuscus (Koi), Heteropneustes fossilis (Shing) and Mystus vittatus (Tengra) fishes were collected and subjected to form an in vitro biofilm by shaking condition into the wise bath. The drug-resistant pattern was determined by the Kirby Bauer technique. Results: All the samples exhibited a huge array (up to 10(7) cfu/ml or g) of bacteria such as E. coli, Klebsiella spp., Vibrio spp., Salmonella spp., Proteus spp. and Staphylococcus spp. The isolates from both the bulk samples and their corresponding biofilms were subjected to antibiogram assay using antibiotics such as Ampicillin (10 mu g), Erythromycin (15 mu g), Streptomycin (STP 10 mu g), Oxacillin (10 mu g), Nalidixic acid (30 mu g). Before biofilm formation, few of the isolates were found to be sensitive and few were resistant against the antibiotics. But when the species were isolated from the biofilm the sensitive one acquired drug resistance and resistant strain unveiled more resistance towards the same antibiotics. The present study revealed extensive bacterial contamination in fish samples among those some were resistant against the supplied drugs. Conclusion: After the formation of multi-species biofilm, the isolates became more resistant against the same drugs that is alarming for consumers and major obstacles to maintain sustainable health. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.Peer reviewe

GUT SYMPTOMS LINKED WITH COVID-19 : A SYSTEMATIC REVIEW

The most prevalent symptoms at the onset of COVID-19 are fever, cough, fatigue, myalgia, and dyspnea (shortness of breath). Initially, it was thought that the virus only causes respiratory distress in patients until the viral RNA has been detected in the patient's stool. Recently, several new studies have depicted that COVID-19 has impact on gut patients. We hypothesized that, there may have a link between gut symptoms and COVID-19. Therefore, the present study was reviewed to explore this study question; searches were conducted to identify the articles related to the association between gut symptoms and COVID-19, which were published between 2019 to 2020. Multiple searches were conducted in Google Scholar and ResearchGate using keywords. In this review, a total of 2639 cases of COVID-19 from 20 articles had been analyzed with special emphasize on gut symptoms. Among 20 studies, Diarrhea (highest 71.62% and lowest 2%) was the most prevalent symptoms, respectively, nausea (highest 17.1% and lowest 1%) & vomiting (highest 16.7% and lowest 1%), anorexia (highest 66.7% and lowest 17.9%), and abdominal pain (highest 8.8% and lowest 1.9%). Along with the main symptoms, we studied some commonly associated symptoms, such as, fever (highest 98.6% and lowest 55.6%) and coughing (highest 91.67% and lowest 35%) were heavily linked with COVID 19. Despite all the GI symptoms associated with COVID 19, there are currently no recommendations for a diagnostic approach in the presence of gastrointestinal symptoms associated with the corona virus, and there is no definitive knowledge of the role of COVID-19 in the gastrointestinal diseases. So, further studies are needed to identify the better relationship between gut symptoms and SARS-CoV-2 for suppressing the spread of COVID-19.Peer reviewe

Impact of gut microbiome on skin health : gut-skin axis observed through the lenses of therapeutics and skin diseases

Publisher Copyright: © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.The human intestine hosts diverse microbial communities that play a significant role in maintaining gut-skin homeostasis. When the relationship between gut microbiome and the immune system is impaired, subsequent effects can be triggered on the skin, potentially promoting the development of skin diseases. The mechanisms through which the gut microbiome affects skin health are still unclear. Enhancing our understanding on the connection between skin and gut microbiome is needed to find novel ways to treat human skin disorders. In this review, we systematically evaluate current data regarding microbial ecology of healthy skin and gut, diet, pre- and probiotics, and antibiotics, on gut microbiome and their effects on skin health. We discuss potential mechanisms of the gut-skin axis and the link between the gut and skin-associated diseases, such as psoriasis, atopic dermatitis, acne vulgaris, rosacea, alopecia areata, and hidradenitis suppurativa. This review will increase our understanding of the impacts of gut microbiome on skin conditions to aid in finding new medications for skin-associated diseases.Peer reviewe

海藻Padina属の分泌する水溶性物質であるプロゲステロン膜受容体(ｍPR)反応性天然化合物の精製とその性状解析

博士（理学）創造科学技術大学院静岡大学甲第1239号non

Purification and characterization of water soluble natural compounds from the marine algae Padina that interact with the membrane progesterone receptor (mPR)

博士（理学）創造科学技術大学院静岡大学甲第1239号non

Screening the in-vitro Antibacterial Activity of Different Naturally Produced Coal and Coal Mediated Commercial Products on Clinically Isolated Pathogens

Background: The rise of microbial illnesses caused by drug-resistant microflora is one of the most serious public health concerns, therefore identifying novel antimicrobial agents is crucial in combating multidrug-resistant bacterial strains. Methods: Present Investigation designated to assess the microbiological status of some naturally produced coal and coal mediated commercial products collected from Barapukuria Coal Mine Dinajpur and different retailer in Dhaka city, respectively through conventional cultural and biochemical tests. Moreover, the anti-bacterial potential of both categories of coal samples was also aimed to be checked by the agar well diffusion method and minimal inhibitory concentration (MIC) assay. Results: Out of 10 samples, total viable bacteria was estimated at high range in all the samples excluding the charcoal. In case of specific bacteria, only Staphylococcus spp. was found in both types of samples while Klebsiella spp. was found in coal mediated commercial products. Both categories of samples showed their anti-bacterial activity with satisfactory range of zone diameter (9 mm–26 mm). However, the naturally produced coal was unable to show their anti-bacterial activity against Bacillus spp., Shigella spp. and Vibrio spp. through agar well diffusion methods. Meanwhile, the minimal concentration of all the samples was successfully quantified as MIC value at which the bacterial growth was retarded. In case of naturally produced coal, the lowest concentration 4 mg/ml was recorded for wooden coal powder against Escherichia coli while the maximum concentration was estimated at 34 mg/ml. Conversely, the MIC value was varied between 4 mg/ml to 24 mg/ml in case of coal mediated commercial products. Conclusion: The results of this study suggest that the naturally produced coal and coal-mediated products have excellent antibacterial characteristics which indicating their economic potential for treating various infectious disorders

Additional file 1: of Assessment of survival of pathogenic bacteria in fresh vegetables through in vitro challenge test

Growth decay and survival capacity of the test isolates in “Chilli, Onion, Capsicum and Coriander”. (DOC 69 kb

In silico prediction of siRNA to silence the SARS-CoV-2 omicron variant targeting BA.4, BA.5, BQ.1, BQ1.1. and XBB: an alternative to traditional therapeutics

Abstract Background After the first infection in December 2019, the mutating strains of SARS-CoV2 have already affected a lot of healthy people around the world. But situations have not been as devastating as before the first pandemic of the omicron strains of SARS-CoV2. As of January 2023, five more Omicron offshoots, BA.4, BA.5, B.Q.1, B.Q.1.1 and XBB are now proliferating worldwide. Perhaps there are more variants already dormant that require only minor changes to resurrect. So, this study was conducted with a view to halting the infection afterwards. The spike protein found on the virus outer membrane is essential for viral attachment to host cells, thus making it an attractive target for vaccine, drug, or any other therapeutic development. Small interfering RNAs (siRNAs) are now being used as a potential treatment for various genetic conditions or as antiviral or antibacterial therapeutics. Thus, in this study, we looked at spike protein to see if any potential siRNAs could be discovered from it. Results In this study, by approaching several computational assays (e.g., GC content, free energy of binding, free energy of folding, RNA–RNA binding, heat capacity, concentration plot, validation, and finally molecular docking analysis), we concluded that two siRNAs could be effective to silence the spike protein of the omicron variant. So, these siRNAs could be a potential target for therapeutic development against the SARS-CoV2 virus by silencing the spike protein of this virus. Conclusion We believe our research lays the groundwork for the development of effective therapies at the genome level and might be used to develop chemically produced siRNA molecules as an antiviral drug against SARS-CoV2 virus infection. Graphical abstrac

Two-Step Mechanism of Cyclin B Degradation Initiated by Proteolytic Cleavage with the 26 S Proteasome in Fish

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