‘Mild Mannered’? Protest and Mobilisation in Portugal under Austerity, 2010–2013

Anti-austerity mobilisations in Southern Europe since 2010 have been widely debated in recent times. Commentators have emphasised the emergence of new political subjects such as the ‘precariat’ organised into loose, IT-connected movements. To what extent do these portrayals reflect the underlying dynamics of this protest cycle, and how do these movements interact with traditional political actors? Using Portugal as a case study, this article maps the cycle of anti-austerity contention between 2010 and 2013 to reveal a more complex picture, where traditional actors, including labour unions and left-wing political parties, emerge as key actors, facilitating and sustaining the discontinuous mobilisation of new forms of activism, while seeking to gain access to new constituencies through them.This is the accepted manuscript. The final version is available from Taylor & Francis at http://www.tandfonline.com/doi/abs/10.1080/01402382.2014.937587#.VGHwa4XziEo

‘Mild mannered’? Protest and mobilisation in Portugal under austerity, 2010–2013

Anti-austerity mobilisations in Southern Europe since 2010 have been widely debated in recent times. Commentators have emphasised the emergence of new political subjects such as the ‘precariat’ organised into loose, IT-connected movements. To what extent do these portrayals reflect the underlying dynamics of this protest cycle, and how do these movements interact with traditional political actors? Using Portugal as a case study, this article maps the cycle of anti-austerity contention between 2010 and 2013 to reveal a more complex picture, where traditional actors, including labour unions and left-wing political parties, emerge as key actors, facilitating and sustaining the discontinuous mobilisation of new forms of activism, while seeking to gain access to new constituencies through them.info:eu-repo/semantics/acceptedVersio

Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection

Circulating skeletal muscle related microRNAs profile in Piedmontese cattle during different age

Abstract Piedmontese cattle is known for double-muscle phenotype. MicroRNAs (miRNAs) play important role as regulators in skeletal muscle physiological processes, and we hypothesize that plasma miRNAs expression profiles could be affected by skeletal muscle growth status related to age. Plasma samples of cattle were collected during four different ages from first week of life until the time of commercial end of the fattening period before slaughter. Small-RNA sequencing data analysis revealed the presence of 40% of muscle-related miRNAs among the top 25 highly expressed miRNAs and, 19 miRNAs showed differential expression too. Using qRT-PCR, we validated in a larger bovine population, miRNAs involved in skeletal muscle physiology pathways. Comparing new-born with the other age groups, miR-10b, miR-126-5p, miR-143 and miR-146b were significantly up-regulated, whereas miR-21-5p, miR-221, miR-223 and miR-30b-5p were significantly down-regulated. High expression levels of miR-23a in all the groups were found. Myostatin, a negative regulator of skeletal muscle hypertrophy, was predicted as the target gene for miR-23a and miR-126-5p and we demonstrated their direct binding. Correlation analysis revealed association between miRNAs expression profiles and animals’ weights along the age. Circulating miRNAs could be promising for future studies on their biomarker potentialities to beef cattle selection

'Mild mannered'? Protest and mobilization in Portugal under austerity, 2010-2013

Em tempos recentes tem-se debatido muito as mobilizações anti-austeridade ocorridas na Europa do Sul desde 2010. Os comentadores frisaram a emergência de novos atores políticos, tais como o «precariado», organizados em movimentos pouco estruturados com base nas tecnologias de informação e comunicação. Até que ponto é que estes movimentos refletem a dinâmica subjacente deste ciclo de protestos e como é que interagem com os atores políticos tradicionais? Utilizando Portugal como um caso-estudo, este artigo traça o mapa das ações políticas reivindicativas anti-austeridade entre os anos de 2010 e 2013, revelando uma panorâmica mais complexa, onde os atores tradicionais, incluindo os sindicatos e os partidos políticos de esquerda, emergem como atores-chave, facilitando e nutrindo a mobilização intermitente de novas formas de ativismo, procurando ao mesmo tempo ganhar com elas acesso a novas clientelas eleitorais.info:eu-repo/semantics/publishedVersio

A biologically inspired neural network controller for ballistic arm movements

<p>Abstract</p> <p>Background</p> <p>In humans, the implementation of multijoint tasks of the arm implies a highly complex integration of sensory information, sensorimotor transformations and motor planning. Computational models can be profitably used to better understand the mechanisms sub-serving motor control, thus providing useful perspectives and investigating different control hypotheses. To this purpose, the use of Artificial Neural Networks has been proposed to represent and interpret the movement of upper limb. In this paper, a neural network approach to the modelling of the motor control of a human arm during planar ballistic movements is presented.</p> <p>Methods</p> <p>The developed system is composed of three main computational blocks: 1) a parallel distributed learning scheme that aims at simulating the internal inverse model in the trajectory formation process; 2) a pulse generator, which is responsible for the creation of muscular synergies; and 3) a limb model based on two joints (two degrees of freedom) and six muscle-like actuators, that can accommodate for the biomechanical parameters of the arm. The learning paradigm of the neural controller is based on a pure exploration of the working space with no feedback signal. Kinematics provided by the system have been compared with those obtained in literature from experimental data of humans.</p> <p>Results</p> <p>The model reproduces kinematics of arm movements, with bell-shaped wrist velocity profiles and approximately straight trajectories, and gives rise to the generation of synergies for the execution of movements. The model allows achieving amplitude and direction errors of respectively 0.52 cm and 0.2 radians.</p> <p>Curvature values are similar to those encountered in experimental measures with humans.</p> <p>The neural controller also manages environmental modifications such as the insertion of different force fields acting on the end-effector.</p> <p>Conclusion</p> <p>The proposed system has been shown to properly simulate the development of internal models and to control the generation and execution of ballistic planar arm movements. Since the neural controller learns to manage movements on the basis of kinematic information and arm characteristics, it could in perspective command a neuroprosthesis instead of a biomechanical model of a human upper limb, and it could thus give rise to novel rehabilitation techniques.</p

Human Milk Protein Production in Xenografts of Genetically Engineered Bovine Mammary Epithelial Stem Cells

BACKGROUND: In the bovine species milk production is well known to correlate with mammary tissue mass. However, most advances in optimizing milk production relied on improvements of breeding and husbandry practices. A better understanding of the cells that generate bovine mammary tissue could facilitate important advances in milk production and have global economic impact. With this possibility in mind, we show that a mammary stem cell population can be functionally identified and isolated from the bovine mammary gland. We also demonstrate that this stem cell population may be a promising target for manipulating the composition of cow's milk using gene transfer. METHODS AND FINDINGS: We show that the in vitro colony-forming cell assay for detecting normal primitive bipotent and lineage-restricted human mammary clonogenic progenitors are applicable to bovine mammary cells. Similarly, the ability of normal human mammary stem cells to regenerate functional bilayered structures in collagen gels placed under the kidney capsule of immunodeficient mice is shared by a subset of bovine mammary cells that lack aldehyde dehydrogenase activity. We also find that this activity is a distinguishing feature of luminal-restricted bovine progenitors. The regenerated structures recapitulate the organization of bovine mammary tissue, and milk could be readily detected in these structures when they were assessed by immunohistochemical analysis. Transplantation of the bovine cells transduced with a lentivirus encoding human β-CASEIN led to expression of the transgene and secretion of the product by their progeny regenerated in vivo. CONCLUSIONS: These findings point to a common developmental hierarchy shared by human and bovine mammary glands, providing strong evidence of common mechanisms regulating the maintenance and differentiation of mammary stem cells from both species. These results highlight the potential of novel engineering and transplant strategies for a variety of commercial applications including the production of modified milk components for human consumption