Clinical Use of OCT in the Management of Epiretinal Membranes

Epiretinal membranes (ERM) are frequently seen in an aging eye, especially after posterior vitreous detachment, and can cause decreased vision, and/or metamorphopsia. Not all of the ERMs detected in routine ophthalmological examination are indicated to be removed with surgery. Optical coherence tomography (OCT) examination reveals the microanatomy of all the retinal layers and enables the vitreoretinal surgeon to make decision to follow-up or to perform a vitrectomy to peel the ERM. OCT imaging clearly shows all the tractions on the retina and the intraretinal layers; and can have a prognostic value for the surgery. OCT imaging is also very valuable in the differential diagnosis of pseudoholes with macular and lameller holes; much better than the clinical examination. It is a routine part of the detailed retinal examination of an eye with an ERM. This chapter covers OCT findings in ERMs, and examples of cases with ERMs indicated for surgery of follow-up will be shown, explaining the clinical results of the cases

Farklı İBA konsantrasyonları ve bekletme sürelerinde bozkır otu çeliklerinin çoğaltılması

Forage kochia, a subshrub forage plant, grows naturally in the pastures of dry areas in Turkey. Their seeds grow slowly in nature, and being short duration of seed viability could be caused by limitations on the cultivation of forage kochia in Turkey. Forage kochia seedlings can be produced serially quickly with cutting propagation as vegetation propagation method. Therefore, forage kochia cuttings were exposed to 12 different IBA concentrations (250, 500, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 10000, and 15000 mg kg-1 ) at four holding times (5, 30, 180, and 900 s) in this research carried out Completely Randomized Design in the greenhouse. We investigated the percentage of rooting, the number of the root, shoot and root length, shoot and root weight, dry root weight, and leave yellowing of these cutting. The best developments in the percentage of rooting (100%), number of roots (> 7), shoot length (> 20 cm), root length (>10 cm), root weight (>0.60 g), dry root weight (>0.1 g) and leave yellowing (score >1.60) were obtained from 500 mg kg-1 IBA at 900 s, 5000 mg kg-1 IBA at 180 s, and 10000 mg kg-1 IBA at 5 s interactions. As a result, we advised 500 mg kg-1 IBA at 900 s, 5000 mg kg-1 IBA at 180 s and 10000 mg kg-1 IBA at 5 s for the quick and economically cutting propagation of forage kochia.Yarı çalı bir yem bitkisi olan bozkır otu, Türkiye’nin kurak alanlarındaki meralarda doğal olarak yetişmektedir. Bozkır otunun doğada tohum ile büyümesi çok yavaş ve tohum canlılığı süresinin kısa olması ülkemizde bozkır otu tarımında kısıtlamaya neden olmaktadır. Vejetatif çoğaltma yöntemi olarak çelikle çoğaltma ile bozkır otu fidesi daha kısa sürede seri bir şekilde üretilebilir. Bu sebeple, Tesadüf Parselleri Deneme Desenine göre serada yürütülen bu çalışmada 4 adet sürede (5, 30, 180 ve 900 s) ve 12 farklı IBA konsantrasyonlarına (250, 500, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 10000 ve 15000 mg kg-1 ) bozkır otu çelikleri maruz bırakıldı. Bu çeliklerin köklenme yüzdesi, kök sayısı, fide ve kök uzunluğu, yaş fide ve kök ağırlığı, kuru kök ağırlığı ve yaprak sararması incelenmiştir. Çeliklerde köklenme yüzdesi (%100), kök sayısı (>7 adet), fide uzunluğu (>20 cm), kök uzunluğu (>10 cm), kök ağırlığı (>0.60 g), kuru kök ağırlığı (>0.1 g) ve yaprak sararması (Skor >1.60) açısından en iyi gelişim 900 saniyede 500 mg kg-1 IBA, 180 saniyede 5.000 mg kg-1 IBA ve 5 saniyede 10.000 mg kg-1 IBA konsantrasyonlarından elde edilmiştir. Sonuç olarak bozkır otunun ekonomik ve hızlı bir şekilde çelikle çoğaltılması için 900 saniyede 500 mg kg-1 IBA, 180 saniyede 5.000 mg kg-1 IBA ve 5 saniyede 10.000 mg kg-1 IBA konsantrasyonları önerilmektedir

The Determination of Botanical Properties of Forage Kochia Population Grown in Konya Conditions

Adverse soil and environmental factors cause a decrease in pasture yield in our country. Shrub species are given importance in breeding studies carried out in order to increase the yield in marginal pastures in the world. Forage kochia (Kochia prostrata), which is a naturally growing and semi-shrub in Turkey%252339%253Bs flora, shows tolerance to adverse soil and climatic conditions. This research was established in Konya in October 2017 according to the Randomized Complete Block Design with 4 replications. In the research, the morphological and yield values of the forage kochia populations collected from 5 different locations in Konya (i.e., Karapınar Kartal Kayaları, Bahri Dağdaş I.A.R.I, Campus Beltway-Selçuklu, Ardıçlı Rural- Selçuklu, and S.U.F.A. Forage Kochia Demonstration Garden) were examined during 2018-2019. We investigated the blooming time (Scoring), plant height (cm), canopy diameter (cm), number of branch, stem diameter (mm), shape of habitus (Scoring), leaf length (mm), leaf width (mm), color of anther and stigma (Scoring), fodder yield per plant (g) and hay yield per plant (g). In this study, the Campus Beltway- Selçuklu Population (3P) bloomed the earliest in this area between the end of August and early September (Score 5,36). Among the forage kochia populations showing semi-decumbent habitus (Score 7,05-7,63) the Karapınar Kartal Kayaları Population (1P) had the highest yield potential regarding plant height (i.e., 46,63 cm), canopy diameter (i.e., 50,50 cm), fodder yield per plant (i.e., 112 g), and hay yield per plant (i.e., 45,28 g). In line with the findings obtained in the study, while the Karapınar Kartal Kayaları Population (1P) and the Campus Beltway- Selçuklu Population (3P) stand out in terms of yield and yield components. These results show us that each population is a valuable gene resource in plant breeding for pasture improvement

The study of urban form as an Archipelago : the case of Ankara

Since the founding of the Turkish Republic, Ankara has been an experimental city that became a laboratory for urban production in line with the ideals of the republic. This experimentation resulted in multifarious urban forms. The initial emphasis of this research is to extract autonomous urban forms of Ankara as a city built with a set of aggregations on a fluvial land that has been transformed into an ambiguous entity remarkably varied in the form in time. To understand episodes of the city based on geography, it is crucial to founding a dialogue between the morphological layers of the city and the elevation of the topography. To be able to develop this kind of dialogue this paper aims to reread the city by referring to the concept of "archipelago" as an analytical tool from a typo-morphological perspective. The word archipelago is referred by O.M.Ungers and Rem Koolhaas to describe typologically the ensemble of self-sufficient built urban forms that are delimited via a common ground. In the case of Ankara, the common ground appears as the distinctive topography consisting of valley floors shaping the physical pattern of the city. A qualitative morphological decomposition method is pursued to generate a catalogue of types in several layers, mainly building footprints, street systems, and topography, in order to achieve three-dimensional morphological analysis. In accordance with this decomposition, a character mapping results in categorizing the city form as follows: gated enclaves, stacks, and objects. In addition to the extraction, pursuing two major trajectories as landform and urban form in the character mapping leads to the exploration of analogies on the urban scale

Content Analysis of College Students' Autobiographical Memories about Circumcision

This study examined college students' autobiographical memories about circumcision by using the content analysis method and laid out the main themes as social and cultural experiences, medical elements, religious elements, manhood, emotionally positive experiences, emotionally negative experiences, and reference to past/time. After content analysis was completed, chi-square tests were run and the most prominent theme in circumcision memories were found to be social and cultural experiences (χ2 = 25.37, p < .001). In order to compare the frequency of emotionally positive and negative experiences in circumcision memories, chi-square test was run and it was found that while college students were narrating their autobiographical memories about circumcision, they referred to emotionally negative experiences more frequently (χ2 = 52.32, p < .001). In conclusion, in this study the participants were found to remember their circumcision memories most frequently within the context of social and cultural practices, and usually with negative emotions; and in this way the content landscape of circumcision experience was laid out

Investigation of Wheat Germ and Oil Characteristics with Regard to Different Stabilization Techniques

Pozadina istraživanja. Upotreba pšeničnih klica i njihovog ulja ograničena je zbog velike aktivnosti enzima u klicama i prisutnosti nezasićenih masnih kiselina u ulju, što zahtijeva primjenu stabilizacijskih tehnika. Eksperimentalni pristup. U ovom su istraživanju procijenjeni učinci metoda stabilizacije (suho zagrijavanje u konvekcijskoj pećnici na 90 i 160 °C, mikrovalno zračenje na 180 i 360 W i uparavanje u autoklavu) na pšenične klice i njihovo ulje. Rezultati i zaključci. Uparavanje je prouzročilo najdramatičnije promjene aktivnosti lipoksigenaze, udjela slobodnih masnih kiselina, aktivnosti uklanjanja radikala DPPH i masenih udjela tokoferola i tokotrienola. Niži peroksidni broj izmjeren je u uzorcima ulja obrađenim konvekcijskim zagrijavanjem (160 °C) i parom na temperaturama iznad 100 °C. Međutim, vrijednosti p-anisidina u uzorcima tretiranim na višim temperaturama bile su znatno veće od onih u uzorcima stabiliziranim pri nižim temperaturama. Zagrijavanje u pećnici na 160 °C je nakon uparavanja bilo jedno od najučinkovitijih postupaka za inaktivaciju lipoksigenaze. Primjena pare bitno je smanjila maseni udjel ukupnih tokoferola, što je izravno povezano s povećanim gubitkom β-tokoferola. Suprotno tome, pronađeno je znatno više γ- i δ-tokoferola te homologa tokotrienola u uparenim uzorcima. Izomeri najotporniji na procese stabilizacije bili su α-tokoferol i γ-tokotrienol. Novina i znanstveni doprinos. Ovo istraživanje pokazuje da metoda zagrijavanja u pećnici na visokoj temperaturi, koja se često koristi u industriji za toplinsku stabilizaciju pšeničnih klica, ne poboljšava njihovu oksidacijsku stabilnost u usporedbi s obradom parom i mikrovalovima. Uparavanje odgađa oksidacijske procese u klici te inhibira aktivnost lipoksigenaze, a osim toga smanjuje gubitak tokotrienola. Za industrijsku se primjenu preporučuje uporaba mikrovalne pećnice male snage (180 umjesto 360 W) i zagrijavanje u pećnici na nižoj temperaturi (90 umjesto 160 °C).Research background. Utilization of wheat germ and wheat germ oil is limited due to high enzymatic activity and the presence of unsaturated fatty acids, which require stabilization techniques to overcome this problem. Experimental approach. In this study, the effects of stabilization methods (dry convective oven heating at 90 and 160 °C, microwave radiation at 180 and 360 W, and autoclave steaming) on both wheat germ and its oil were evaluated. Results and conclusions. Steaming caused the most dramatic changes in lipoxygenase activity, free fatty acid content, DPPH radical scavenging activity, and mass fractions of tocopherols and tocotrienols. Lower peroxide values were measured in the oil samples treated with convectional heating (160 °C) and steaming at temperatures above 100 °C. However, p-anisidine values of samples treated at higher temperatures were considerably greater than those of samples stabilized at lower temperatures. Oven heating at 160 °C was also one of the most effective treatments, after steaming, for the inactivation of lipoxygenase. Steaming significantly reduced mass fraction of total tocopherols, which was directly associated with the greater loss of β-tocopherol content. On the contrary, γ- and δ-tocopherol and tocotrienol homologues were abundant with higher amounts in steamed samples. α-Tocopherol and γ-tocotrienol were the most resistant isomers to stabilization processes. Novelty and scientific contribution. This study shows that the high temperature oven heating method, which is widely used in the industry for thermal stabilization of wheat germ, does not provide an advantage in oxidative stability compared to steaming and microwave applications. Steaming delayed oxidation in the germ, while further inhibiting lipoxygenase activity. Moreover, tocotrienols were more conservable. In industrial application, low-power microwave (180 instead of 360 W) and oven heating at lower temperature (90 instead of 160 °C) would be preferable

Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus

Abstract Background/ Aims: Sjögren’s syndrome (SS) and diabetes mellitus (DM) are common causes of dry eye disease (DED) and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the healthy individuals. Methods: Twenty-three SS patients (mean age 52.70±7.55 years), 25 DM patients (mean age 56.68±6.53 years), and 23 healthy subjects (mean age 51.70±9.14 years) were included in the study. After detailed ophthalmological examination, Schirmer test, tear break-up time (TBUT) and Ocular Surface Disease Index (OSDI) scores were measured. Serum levels of six different proinflammatory interleukins (IL), five growth factors, matrix metalloproteinase-9, and fibronectin were measured by immunoassay. One-way ANOVA or Kruskal-Wallis tests and Dunn-Bonferroni post hoc analysis were used for comparison and p&lt;0.05 was considered significant. Results: Schirmer test and TBUT were significantly lower in the SS group (2.08±1.72 mm/5 min and 3.08±2.08 s) than in the DR (10.24±4.63 mm/5 min and 4.20±3.09 s) and control groups (13.30±5.95 mm/5 min and 9.00±1.75 s) (p&lt;0.001). Among the parameters studied, mean serum IL-23 level was significantly higher in the SS group (156.66±207.94 pg/mL) than in the DM and control groups (73.48±95.91 and 69.59±105.39 pg/mL, respectively) (p&lt;0.05). Serum insulin-like growth factor 1 (IGF-1) level was lowest in DM patients (DM: 12.89±21.09, SS: 30.77±19.85, and control: 27.08±21.93 ng/mL) (p&lt;0.05). Sjögren’s syndrome disease activity index (ESSDAI) showed a negative correlation with TBUT and a positive correlation with IL-1, IL-2 and fibronectin (p&lt;0.005). Conclusions: Except IL-23 and IGF-1, the contents of serum obtained from patients with SS and DM are similar with the healthy individuals. Therefore, autologous serum seems to be a good option to replace deficient tear fluid in these subjects

Examination of emotion regulation in the family context: A mixed method study from Turkey

Individuals use different strategies dealing with their negative emotions and these strategies are associated with various adjustment outcomes. In the family context, parental emotion regulation strategies are also linked to the children’s emotion regulation. One aim of the current study was to examine the type of emotion regulation strategies used by parents in the Turkish cultural context. Another aim was to exploratorily investigate whether parents’ emotion regulation processes emerge in a way that predicts their children’s emotion dysregulation. One hundred seven families that had a child attending primary school participated in this study. We conducted semi-structured interviews with the parents at their homes. Child emotion dysregulation was also measured with the Emotion Regulation Checklist. Based on content analysis, the findings revealed four main themes showing a converging pattern among parents, namely non-confrontation, proactive strategies, metacognitive strategies, and dysregulated expression. In the subsequent quantitative part of the study, parental adaptiveness scores for emotion regulation strategies were calculated to examine relationships with children’s emotion dysregulation. The findings of separate hierarchical regression analyses for mothers and fathers indicated that the maternal, but not paternal, adaptiveness level in emotion regulation significantly predicted the child’s emotion dysregulation after controlling for parental education and the child’s age and gender. These qualitative and quantitative findings could contribute to the literature by drawing a comprehensive picture of emotion regulation in the family context in Turkey

Bakteriyel Vajinal Mikrobiyotanın Metagenomik Yaklaşımla Tanımlanması

Amaç: Bu çalışmanın amacı, alem düzeyinden tür düzeyine kadar farklı taksonomik seviyelerde yüksek verimli yeni nesil dizileme ve metagenomik yaklaşım kullanarak 38 Türk kadınının vajinal bakteriyel mikrobiyotasını belirlemektir. Gereç ve Yöntem: Yeditepe Üniversitesi Hastanesi Kadın Hastalıkları ve Doğum Kliniği’nde Haziran 2021’de DNA/RNA koruma toplama tüplerine vajinal sürüntü örnekleri (n=38) alındı ve ZymoBIOMICS DNA miniprep kiti ile DNA ekstraksiyonu yapıldı. Hastaların yaşı, medeni durumu, ön tanı ve anamnez durumu ile ilgili bilgiler toplandı. Vajinal mikrobiyotayı belirlemek için 16S rRNA amplikon DNA dizilimi kullanılarak metagenomik bir yaklaşım uygulandı. Bulgular: Vajinal örneklerde baskın filum Firmicutes’i Proteobacteria, Actinobacteria, Tenericutes, Fusobacteria ve Synergistetes izledi. Lactobacillus en fazla bulunan “cins düzeyinde bakteri” olup onu Prevotella, Enterobacter, Gardnerella ve Dialister izledi. Vajinal sürüntü örneklerinde tür düzeyinde Lactobacillus iners baskın bulundu, bunu Gardnerella vaginalis, Enterobacter tabaci, Prevotella timonensis, Prevotella bivia ve Lactobacillus jensenii izledi. Kanonik uyum analizi (CCA), filum düzeyinde Proteobacteria ve Fusobacteria’nın en yüksek yüzdelerle evli/bekar değişkeni ile ilişkili olduğunu, ancak Actinobacteria ve Tenericutes’in yaş değişkeni ile ilişkili olduğunu gösterdi. Campylobacter, Atopobium, Enterobacter ve Lactococcus en yüksek yüzdelerle evli/bekar değişkeni ile ilişkili bulunurken, Anaerococcus, Streptococcus, Sutterella ve Veillonella en yüksek yüzdelerle yaşla ilişkili bulundu. Ayrıca, CCA, Campylobacter ureolyticus, Lb. jensenii ve Atopobium vajinae türlerinin evli/bekar değişkeni ile en yüksek yüzdelerle ilişkilendirirken, Lactobacillus johnsonii ve G. vaginalis en yüksek yüzdelerle yaş değişkeninde ilişkili bulundu. Sonuç: Vajinal hastalıklar hala önemli bir halk sağlığı sorunudur. Son yıllarda teknolojik gelişmeler sayesinde daha derinlemesine çalışılan vajinal mikrobiyotanın sanıldığından daha karmaşık olduğu keşfedilmiştir. Bu bulguları doğrulamak ve geliştirmek için daha fazla hasta araştırmasına ihtiyaç vardır

Alteration of boza microbiota in the fermentation process

Boza is a fermented beverage containing beneficial microorganisms for human health. In our study, microbiota present in raw materials used boza production (corn flour, wheat flour, and mayşe), 1st day, 3rd day of boza fermentation and 4th day final product of boza, has been identified by Next Generation DNA Sequencing and metagenomic analysis. As a result of genus-level analysis directly from corn flour and wheat flour samples contained dominantly Streptophyta and Pleomorphobacterium, while in the 1st day, 3rd day, the final product of boza and boza ferment the dominant bacteria were Leuconostoc and Lactococcus at genus level. In the analysis of the pre-enriched samples, the dominant bacteria in corn flour were Enterococcus, Klebsiella, and Micromonospora and in wheat flour were Pantoea and Bacillus. Boza ferment, boza on the 1st day, boza on the 3rd day and the final product of boza dominantly contained Lactococcus. The bacterial diversity, similarity and differences among samples were analyzed by Principal Coordinate Analysis and dendrogram construction. The contribution of raw materials used in the production of boza change to the products at the fermentation stage and to the microbiota during the fermentation process and their contribution to the final product were determined by metagenomic analysis at DNA level.Boza, insan sağlığı için yararlı mikroorganizmaları içeren fermente bir içecektir. Çalışmamızda boza üretiminde ham madde olarak kullanılan (mısır unu, buğday unu, mayşe) ve boza fermantasyonunun 1. günü, 3. günü ve 4. gün son ürün boza’nın içerdiği mikrobiyota Yeni Nesil DNA Dizileme yöntemi ve metagenomik analiz ile ortaya çıkarılmıştır. Örneklerden doğrudan cins düzeyinde yapılan analiz sonucunda, mısır unu ve buğday ununda dominant olarak Streptophyta ve Pleomorphobacterium bulunurken; bozanın 1. gün, 3. gün ve son ürün ile boza mayasında dominant bakterilerin Leuconostoc ve Lactococcus cinsine ait olduğu tespit edilmiştir. Ön zenginleştirme yapılan örneklerin analizinde, mısır ununda dominant bakteriler Enterococcus, Klebsiella ve Micromonospora, buğday ununda ise Pantoea ve Bacillus olduğu, boza mayası, 1. gün boza, 3. gün boza ve satışa sunulan son üründe dominant bakteri Lactococcus olarak belirlenmiştir. Çalışmamızda örnekler arasındaki bakteriyel çeşitlilik, benzerlik ve farklılıklar Principal Coordinate Analiz ve dendrogram oluşturulması ile ortaya konmuştur. Boza üretiminde kullanılan ham maddelerin bozanın fermantasyon aşamalarındaki ürünler ile fermantasyon sürecinde mikrobiyotasına nasıl değiştiği ve son ürüne olan katkıları, DNA düzeyinde yapılan metagenomik analizler ile belirlenmiştir