Consequences of Design Decisions on Material Waste during Construction Survey of Architects’ Point of View, the Case of Jordan

The price of crude oil is ever increasing in Jordan. Energy is the main and most fundamental source for economy and construction industries. Given the fact that material manufacturing and construction in building sector need certain amounts of energy, which is called “embodied energy”. The increase of energy prices is reflected on material manufacturing and therefore, any waste means energy waste and more environmental pollution. Construction waste minimization and management are considered as indicators for sustainable construction. Construction industry is considered as one of the major pillars in the Jordanian economy; consequently, it contributes to a high percent of the national energy bill among many other sectors. This may be considered one of the major reasons to consider the study of building materials in the Jordanian construction sector. Construction waste increases the cost of building as the energy and natural resources are consumed during manufacturing. The construction industry fundamental aims are to reduce the wastage of construction materials. This study addresses the incidence of material waste in the Jordanian construction industry and sheds light on decision making during the design phase and its effect on material wastage during construction. This study is intended for the material wastage reduction during construction as a tool to reduce construction costs in Jordan and consequently to reduce the oil bill. At the end, this paper points out the design related major causes of material wastage during construction through a questionnaire designed by the authors

How do Wireless Chains Behave? The Impact of MAC Interactions

In a Multi-hop Wireless Networks (MHWN), packets are routed between source and destination using a chain of intermediate nodes; chains are a fundamental communication structure in MHWNs whose behavior must be understood to enable building effective protocols. The behavior of chains is determined by a number of complex and interdependent processes that arise as the sources of different chain hops compete to transmit their packets on the shared medium. In this paper, we show that MAC level interactions play the primary role in determining the behavior of chains. We evaluate the types of chains that occur based on the MAC interactions between different links using realistic propagation and packet forwarding models. We discover that the presence of destructive interactions, due to different forms of hidden terminals, does not impact the throughput of an isolated chain significantly. However, due to the increased number of retransmissions required, the amount of bandwidth consumed is significantly higher in chains exhibiting destructive interactions, substantially influencing the overall network performance. These results are validated by testbed experiments. We finally study how different types of chains interfere with each other and discover that well behaved chains in terms of self-interference are more resilient to interference from other chains

VEGF binding to NRP1 is essential for VEGF stimulation of endothelial cell migration, complex formation between NRP1 and VEGFR2, and signaling via FAK Tyr407 phosphorylation

In endothelial cells, neuropilin-1 (NRP1) binds vascular endothelial growth factor (VEGF)-A and is thought to act as a coreceptor for kinase insert domain-containing receptor (KDR) by associating with KDR and enhancing VEGF signaling. Here we report mutations in the NRP1 b1 domain (Y297A and D320A), which result in complete loss of VEGF binding. Overexpression of Y297A and D320A NRP1 in human umbilical vein endothelial cells reduced high-affinity VEGF binding and migration toward a VEGF gradient, and markedly inhibited VEGF-induced angiogenesis in a coculture cell model. The Y297A NRP1 mutant also disrupted complexation between NRP1 and KDR and decreased VEGF-dependent phosphorylation of focal adhesion kinase at Tyr407, but had little effect on other signaling pathways. Y297A NRP1, however, heterodimerized with wild-type NRP1 and NRP2 indicating that nonbinding NRP1 mutants can act in a dominant-negative manner through formation of NRP1 dimers with reduced binding affinity for VEGF. These findings indicate that VEGF binding to NRP1 has specific effects on endothelial cell signaling and is important for endothelial cell migration and angiogenesis mediated via complex formation between NRP1 and KDR and increased signaling to focal adhesions. Identification of key residues essential for VEGF binding and biological functions provides the basis for a rational design of antagonists of VEGF binding to NRP1

Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration

c-Src activates Ras-MAPK/ERK signaling pathway and regulates cell migration, while trihydrophobin 1 (TH1) inhibits MAPK/ERK activation and cell migration through interaction with A-Raf and PAK1 and inhibiting their kinase activities. Here we show that c-Src interacts with TH1 by GST-pull down assay, coimmunoprecipitation and confocal microscopy assay. The interaction leads to phosphorylation of TH1 at Tyr-6 in vivo and in vitro. Phosphorylation of TH1 decreases its association with A-Raf and PAK1. Further study reveals that Tyr-6 phosphorylation of TH1 reduces its inhibition on MAPK/ERK signaling, enhances c-Src mediated cell migration. Moreover, induced tyrosine phosphorylation of TH1 has been found by EGF and estrogen treatments. Taken together, our findings demonstrate a novel mechanism for the comprehensive regulation of Ras/Raf/MEK/ERK signaling and cell migration involving tyrosine phosphorylation of TH1 by c-Src

A Pilot Study on Developing Mucosal Vaccine against Alveolar Echinococcosis (AE) Using Recombinant Tetraspanin 3: Vaccine Efficacy and Immunology

Humans and rodents become infected with E. multilocularis by oral ingesting of the eggs, which then develop into cysts in the liver and progress an endless proliferation. Untreated AE has a fatality rate of >90% in humans. Tetraspanins have been identified in Schistosoma and showed potential as the prospective vaccine candidates. In our recent study, we first identified seven tetraspanins in E. multilocularis and evaluated their protective efficacies as vaccines against AE when subcutaneously administered to BALB/c mice. Mucosal immunization of protective proteins is able to induce strong local and systemic immune responses, which might play a crucial role in protecting humans against E. multilocularis infection via the intestine, blood and liver. We focused on Em-TSP3, which achieved significant vaccine efficacy via both s.c. and i.n. routes. The adjuvanticity of nontoxic CpG OND as i.n. vaccine adjuvant was evaluated. The widespread expression of Em-TSP3 in all the developmental stages of E. multilocularis, and the strong local and systemic immune responses evoked by i.n. administration of rEm-TSP3 with CpG OND adjuvant suggest that this study might open the way for developing efficient, nontoxic human mucosal vaccines against AE