Robotic additive manufacturing system featuring wire deposition by electric arc for high-value manufacturing

Increasing demand from the high-value manufacturing industries of quality, productivity, efficiency and security aligns with the ambition and driving need for novel automated robotic systems. This paper describes the motivation, design and implementation phases of the SERFOW project (Smart Enabling Robotics driving Free Form Welding). SERFOW is an automated additive manufacturing arc and wire tungsten inert gas (TIG) welding prototype to support industrial manufacturing requirements of the nuclear, aerospace and automotive industry sectors. Key innovations are found in the integration of a 3D vision system with a robotic manipulator to perform automatic free-form fusion welding for the multiple layer additive material build-up required to expand Additive Manufacturing (AM) with minimum human intervention. Welding trials were performed on samples made of Super Duplex stainless steel alloy. Metallographic observations were performed to analyze the porosity distribution and penetration on the material after welding. Also, temperature, feritescope and tensile measurements were performed. The results showed that the welding and AM process performed with the SERFOW cell are within an acceptable quality tolerance range according to the ISO 5817 and the ASME A789 welding standards

Proof of concept for a virtual reality environment used for intervention planning and training in highly radioactive environments

This paper presents a novel way to predict radiation dose using immersive Virtual Reality (VR). The platform allows an assessment of proposed interventions in as much detail and time as required. Its purpose is to give users the maximum amount of agency while in the environment. Workers get a realistic experience practising jobs and supervisors can oversee the expected radiation doses for each intervention. A proof of concept performed and showed the platform returned a comparable result to the real radiation exposure for a predefined route. The errors of the system are dependant on the dose map. With an accurate dose map, the system will produce reliable results

Tricyclic antidepressants inhibit hippocampal α7*and α9α10 nicotinic acetylcholine receptors by different mechanisms

The activity of tricyclic antidepressants (TCAs) at α7 and α9α10 nicotinic acetylcholine receptors (AChRs) as well as at hippocampal α7-containing (i.e., α7*) AChRs is determined by using Ca2+ influx and electrophysiological recordings. To determine the inhibitory mechanisms, additional functional tests and molecular docking experiments are performed. The results established that TCAs (a) inhibit Ca2+ influx in GH3-α7 cells with the following potency (IC50 in µM) rank: amitriptyline (2.7 ± 0.3) > doxepin (5.9 ± 1.1) ~ imipramine (6.6 ± 1.0). Interestingly, imipramine inhibits hippocampal α7* AChRs (42.2 ± 8.5 μM) in a noncompetitive and voltage-dependent manner, whereas it inhibits α9α10 AChRs (0.53 ± 0.05 µM) in a competitive and voltage-independent manner, and (b) inhibit [3H]imipramine binding to resting α7 AChRs with the following affinity rank (IC50 in μM): imipramine (1.6 ± 0.2) > amitriptyline (2.4 ± 0.3) > doxepin (4.9± 0.6), whereas imipramine’s affinity was no significantly different to that for the desensitized state. The molecular docking and functional results support the notion that imipramine noncompetitively inhibits α7 AChRs by interacting with two overlapping luminal sites, whereas it competitively inhibits α9α10 AChRs by interacting with the orthosteric sites. Collectively our data indicate that TCAs inhibit α7, α9α10, and hippocampal α7* AChRs at clinically relevant concentrations and by different mechanisms of action

The urgent need for robust coral disease diagnostics

Coral disease has emerged over recent decades as a significant threat to coral reef ecosystems, with declines in coral cover and diversity of Caribbean reefs providing an example of the potential impacts of disease at regional scales. If similar trends are to be mitigated or avoided on reefs worldwide, a deeper understanding of the factors underlying the origin and spread of coral diseases and the steps that can be taken to prevent, control, or reduce their impacts is required. In recent years, an increased focus on coral microbiology and the application of classic culture techniques and emerging molecular technologies has revealed several coral pathogens that could serve as targets for novel coral disease diagnostic tools. The ability to detect and quantify microbial agents identified as indicators of coral disease will aid in the elucidation of disease causation and facilitate coral disease detection and diagnosis, pathogen monitoring in individuals and ecosystems, and identification of pathogen sources, vectors, and reservoirs. This information will advance the field of coral disease research and contribute knowledge necessary for effective coral reef management. This paper establishes the need for sensitive and specific molecular-based coral pathogen detection, outlines the emerging technologies that could serve as the basis of a new generation of coral disease diagnostic assays, and addresses the unique challenges inherent to the application of these techniques to environmentally derived coral samples