Novel extration techniques of resverattrol from biowaste

The research was partially supported by Russian Science Foundation, grant № 20-66-47017 and by RFBR grant 18-29-12129mk

INNOVATIVE GREEN APPROACH FOR EXTRACTION AND CHARACTERIZATION OF PIPERINE FROM BLACK PEPPER USING RESPONSE SURFACE METHODOLOGY

The work was supported by the Russian Science Foundation, Grant No.20-66-47017

Characterization of low-fat yogurt manufactured with addition of β-glucan as a dietary supplement

Fermented dairy products constitute a vital component of the human diet globally. The nutriceutical advantages of Beta-glucan as a functional bioactive ingredient is considered as an alternative to conventional yoghurt presented in the market [1]. Beta-glucans are polysaccharides which have been suggested to exhibit anti-tumor, immunomodulatory, radioprotective, antioxidant and anti-inflammatory properties and also the ability to lower cholesterol and glucose level in the blood. Fat content is the most critical parameter in yoghurt production. Excessive intake of dietary fat has been reported to cause some health disorders, such as cardiovascular diseases and obesity. Low-fat yoghurts are ideal dietary products for lactose intolerant persons (particularly young children and the elderly)

The potential impact of the microwave treatment on the biotechnological aspects of dairy products

The research work was partly supported by RFBR grant 18-29-12129mk

An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

The extraction efficiency, recovery, quantification, and stability of isoflavones (daidzein, genistein and puerarin) from the wastes /by-products of kudzu roots and soy molasses were evaluated by using natural deep eutectic solvents (NADESs) coupled with ultrasound-assisted extraction. The NADES extraction was compared with the conventional Soxhlet extraction technique, and the extracted isoflavones were quantified using HPLC-UV/Vis. Results showed extractability of puerarin to be significantly increased (p < 0.0001) in proportion to the increased water concentration of NADES (9.7 ± 0.2, 12.6 ± 0.2, and 14.8 ± 0.3 mg/g in NADESs containing 10%, 20%, and 30% water, respectively). Despite having less isoflavone content, kudzu roots and soy molasses extracts obtained using NADES exhibited higher antioxidant activity when compared with methanolic extracts. Further, NADES extracts also markedly reduced the degradation degree of isoflavones. The results of this investigation support the use of green extraction techniques to obtain value-added components like isoflavones from wastes/by-products like that of kudzu roots and soy molasses, which are envisaged to find future potential applications in the food and pharmaceutical industries. © 2022 Elsevier B.V.Russian Science Foundation, RSF: 20–66-47017This work was supported by the Russian Science Foundation RSF (Grant agreement number 20–66-47017 ).The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Elena G. Kovaleva reports financial support was provided by Russian Science Foundation RSF (Grant agreement number 20–66–47017)

Cytotoxic activity of kudzu flower and red clover extracts against cultivated tumor cells

The purpose of the study is to investigate the cytotoxic effect of kudzu flower (Pueraria lobata flowers) and red clover (Trifolium pratense flowers) extracts on cultured tumor cells and normal human cells.Цель исследования - изучение цитотоксического действия экстрактов цветка кудзу (Pueraria lobata flowers) и красного клевера (Trifolium pratense flowers) на культивируемых опухолевых клетках и нормальных клетках человека

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses Against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This work was supported by the Russian Science Foundation, Grant No. 20-66-47017

Astaxantin and Isoflavones Inhibit Benign Prostatic Hyperplasia in Rats by Reducing Oxidative Stress and Normalizing Ca/Mg Balance

Benign prostatic hyperplasia (BPH) is a common pathology among aging men. Despite the broad pharmacological interventions, the available remedies to treat BPH are yet not devoid of side effects. Herbal compounds are suggested to be an alternative option for the BPH treatment. In our study, we evaluated the effect of kudzu isoflavones and astaxanthin on the BPH animal model. The animals were randomly divided into five groups: control; testosterone-induced BPH group; and three BPH-induced groups, which received intragastrically for 28 days finasteride (5 mg/kg) as a positive control, isoflavones (200 mg/kg), and astaxanthin (25 mg/kg). BPH was induced by castration of animals and subsequent subcutaneous injections of prolonged testosterone (25 mg/kg). Prostate index and histology, biochemical parameters, and antioxidant activity were evaluated. A significant decrease in prostate weight, immunohistochemical markers, and normalization of prostate Ca/Mg ratio was found in all treatment groups. Astaxanthin treatment also resulted in decreased epithelial proliferation and normalized superoxide dismutase activity. In conclusion, both isoflavones and astaxanthin inhibited BPH development at a level comparable to finasteride in terms of prostate weight, prostatic epithelium proliferation, and prostate tissue cumulative histology score. These results suggest that isoflavones and especially astaxanthin could serve as a potential alternative therapy to treat BHP. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Funding: This research was funded by Russian Science Foundation, grant number 20-65-47025

Cupping Therapy (Al-Hijamah): An Exploratory Study of Healthcare Professionals Controversial Beliefs and Conceptions, Kingdom of Saudi Arabia

Background: Cupping therapy (Al-Hijamah) is a traditional therapy, supported and practised by people around the world since ancient times. Cupping therapy related to complementary and alternative medicine is associated with controversial, false beliefs and misconceptions. Objective: This study aimed at exploring controversial beliefs and conceptions about cupping therapy among healthcare professionals, Riyadh, Kingdom of Saudi Arabia (KSA). Methods: A cross-sectional study involving 241 healthcare professionals was carried out from January to June 2016. All participants requesting license for practicing cupping therapy underwent one week Al-Hijamah training course at National Center for Complementary and Alternative (NCCAM). Prior to training course, all subjects completed a 23-item predesigned questionnaire related to controversial beliefs and misconceptions concerning cupping therapy. All participants gave written informed consent. Results: Healthcare professionals who participated in the study (n=241) were 93 physicians, 56 physiotherapists, 61 nurses and 31 other specialists. Saudi participants represented 46.1%. 60.6% were adult males with mean clinical experience of 13.0±9.37 years. "Al-Hijamah gets rid of bad blood" was the commonest controversial belief reported by 68.1% of participants. A proportion of participants (31.5%) reported "Al-Hijamah is absolutely safe" and "Al-Hijamah is effective in the treatment of all diseases". The least controversial beliefs reported by 12%, 8.7% and 2.9% of participants were "Cupping can be used for all patients and all diseases”, "Cupping like a blood donation is not different" and "The scarification of cupping (in wet cupping) must be deep", respectively. Conclusion: The explored controversial beliefs and misconceptions related to cupping therapy may impact knowledge, attitude and practice of cupping practitioners. Therefore, all healthcare professionals need to be regularly exposed to a training course targeting these beliefs and misconceptions concerning cupping therapy prior to giving them license for practicing this traditional modality. Continuing research in cupping mechanisms of action is another prioritized area which can provide scientific explanations underlying its efficacy and outcome together with alleviating its false beliefs and misconceptions