13 research outputs found
âLooking Through the Shariah Window" : A Qualitative Study on the Swedish Main BanksÂŽ Opinions about Islamic Banking and How They Develop a New Service.
Detection of dsRNA in Particles of Vicia Cryptic Virus and in Vicia faba Tissues and Protoplasts
Dorsal dartos flap rotation versus suturing tunica albuginea to the pubic periosteum for correction of penile torsion: A prospective randomized study
Ameliorative effect of selenium nanoparticles and fish oil on cisplatin and gamma irradiation-induced nephrotoxicity in male albino rats
Properties of cocksfoot streak and cocksfoot cryptic, two viruses infecting cocksfoot (Dactylis glomerate) in Scotland
A Scottish isolate of cocksfoot streak virus (CSVâS) was found to have flexuous filamentous particles which, in sap of infected cocksfoot plants, had a modal length of 712 nm. It was transmitted from infected to healthy cocksfoot plants in a nonâpersistent manner by Myzus persicae and by mechanical inoculation of infective sap extracts containing an antiâoxidant. Apart from cocksfoot, mechanical inoculation of infective sap succeeded in infecting only four of 22 plant species tested. The infectivity of sap extracts containing 0.2% thioglycerol was lost after heating for 10 min at 55oC but not 50oC, storage at room temperature for 48 but not 24 hours, and after diluting 10â2 to 10â3. Highly purified preparations of CSVâS particles sedimented as a single component with a sedimentation coefficient of 139S and had a buoyant density in rubidium bromide of 1.31 g/cm3. Virus particles were composed of one protein and one ssRNA species with estimated Mr of 31 000 and 3.2 times 106 respectively. In ELISA, an antiserum prepared to CSVâS detected the virus in all aerial parts of infected cocksfoot plants and, when present in the ratio of 1 infected leaf: 1000 healthy leaves. Both CSVâSâinfected and âuninfected cocksfoot also contained a previously undescribed virus with isometric particles c. 30 nm in diameter. This virus, named cocksfoot cryptic virus (CCV), was seedâborne in two cvs of cocksfoot tested and its particles contained two dsRNA species of estimated Mt of 1.14 times 106 and 1.27 times 106. Despite the fact that particles of CSVâS were largely free from CCV particles following exclusion chromatography on agarose beads prior to immunisation, immunoelectron microscopy (IEM) showed that the antiserum prepared to CSVâS also contained some antibodies to CCV. Evidence from IEM suggested a possible distant serological relationship of CCV to ryegrass and beet (BCV 1 or BCV 2, or both) cryptoviruses, all members of subâgroup A of cryptoviruses.</p