A method for studying electron-density-based dynamics of many-electron systems in scaled cylindrical coordinates

The combination of quantum fluid dynamics and density functional theory had led to the formulation of a single time-dependent equation, the generalized nonlinear Schrödinger equation (GNLSE). In this paper, the above GNLSE is written as a nonlinear diffusion-type equation in appropriately scaled cylindrical coordinates and evolved in imaginary time to obtain the electronic energies, densities and other properties of all the noble gas atoms. The close agreement of the values obtained with those from the literature implies that the same method can be used in real time to study the density-based dynamics of many-electron systems in axially symmetric external fields such as intense laser fields, with relatively less computational effort

<b>Critical process parameters optimization for hyperthermostable β amylase production by <i>Bacillus subtilis</i> DJ5 using response surface methodology </b> - doi: 10.4025/actascibiolsci.v36i1.17427

The combined effects of significant physical and chemical factors affect hyperthermostable β amylase production under submerged fermentation by Bacillus subtilis DJ5. The above was studied using the experimental design and response surface methodology. A 23 full-factorial central composite design was chosen to analyze interactions among three factors i.e. substrate concentration, medium pH and incubation temperature. The experimental data were fitted into a polynomial model for the yield of enzyme and an optimum level was arrived at with optimized conditions. Solving the coded values using Excel equation function indicated that maximum enzyme production is possible at a substrate concentration of 7.07 mg mL-1, pH 6.622 and temperature of 35.435°C. Such prediction was validated with practical experiments in which, at the prescribed condition maximum yield of 15.62 U mg-1, nearly 1.5 fold higher than non-optimized condition was observed

Polar lipid profile of strain JS1^T and its closely related species.

<p>Strains: 1, <i>A</i>. <i>suryakundensis</i> JS1^T; 2, <i>A</i>. <i>flavithermus</i> subsp. <i>yunnanensis</i> DSM 23293^T; 3, <i>A</i>. <i>mongoliensis</i> DSM 19169^T; 4, <i>A</i>. <i>flavithermus</i> subsp. <i>flavithermus</i> DSM 2614^T; 5, <i>A</i>. <i>eryuanensis</i> KCTC 13720^T; 6, <i>A</i>. <i>tengchongensis</i> KCTC 13721^T; 7, <i>A</i>. <i>thermarum</i> DSM 17141^T; 8. <i>A</i>. <i>ayderensis</i> NCIMB 13972^T; 9, <i>A</i>. <i>kamchatkensis</i> DSM 14988^T; 10, <i>A</i>. <i>salavatliensis</i> DSM 22626^T; 11, <i>A</i>. <i>kestanbolensis</i> NCIMB 13971^T; 12, <i>A</i>. <i>contaminans</i> DSM 15866^T; 13, <i>A</i>. <i>gonensis</i> NCIMB 13933^T; 14, <i>A</i>. <i>voinovskiensis</i> DSM 17075^T; 15, <i>A</i>. <i>kaynarcensis</i> LMG 25303^T.</p

Neighbour-joining phylogenetic tree showing the position of <i>Anoxybacillus</i><i>suryakundensis</i> strain JS1^T among the related taxa based on 16S rRNA secondary structure information.

<p><i>E</i>. coli 16S rRNA sequence has been selected as reference template. Bootstrap values expressed as percentages of 1000 replications are given at branch points. Accession numbers are given in parentheses. Bar 2 substitutions per 100 nucleotide position.</p

<i>Anoxybacillus suryakundensis</i> sp. nov, a Moderately Thermophilic, Alkalitolerant Bacterium Isolated from Hot Spring at Jharkhand, India

<div><p>Four closely related facultative anaerobe, moderately thermophilic, Gram positive rods (JS1^T, JS5, JS11, and JS15) were isolated from sediment samples from a hot spring at Suryakund, Jharkhand, India. Colonies were pale yellow, rough surface with uneven edges on TSA after 72 h incubation. Heterotrophic growth was observed at 40-60°C and pH 5.5-11.5; optimum growth occurred at 55°C and pH 7.5. 16S rRNA gene sequence analysis revealed the strains belong to genus <i>Anoxybacillus</i>. DNA-DNA homology values among strains were above 70% and showed distinct ERIC and REP PCR profile. On the basis of morphology and biochemical characteristics, strain JS1^T was studied further. Strain JS1^T showed 99.30% sequence similarity with <i>A. flavithermus</i> subsp. <i>yunnanensis</i>, 99.23% with <i>A. mongoliensis</i>, 99.16% with <i>A. eryuanensis</i>, 98.74% with <i>A. flavithermus</i> subsp. <i>flavithermus</i>, 98.54% with <i>A. tengchongensis</i>, 98.51% with <i>A. pushchinoensis</i>, 97.91% with <i>A. thermarum</i>, 97.82% with <i>A. kaynarcensis</i>, 97.77% with <i>A. ayderensis</i> and <i>A. kamchatkensis</i>, 97.63% with <i>A. salavatliensis</i>, 97.55% with <i>A. kestanbolensis</i>, 97.48% with <i>A. contaminans</i>, 97.27% with <i>A. gonensis</i> and 97.17% with <i>A. voinovskiensis</i>. In 16S rRNA secondary structure based phylogenetic comparison, strain JS1^T was clustered with <i>Anoxybacillus eryuanensis</i>, <i>A. mongoliensis</i>, and <i>A. flavithermus</i> subsp. <i>yunnanensis</i> and showed 15 species specific base substitutions with maximum variability in helix 6. Moreover, DNA-DNA relatedness between JS1^T and the closely related type strains were well below 70%. The DNA G+C content was 42.1 mol%. The major fatty acids were C_{15:0 iso}, C_{16:0 iso} and C_17:0iso. The polar lipids were a phosphatidylgylycerol, a diphosphatidylglycerol, a phosphatidylethnolamine, a phosphatidylcholine, a phosphatidyl monomethylethanolamine and four unknown lipids. Based on polyphasic approach, strain JS1^T represent a novel species of the genus <i>Anoxybacillus</i> for which <i>Anoxybacillus suryakundensis</i> sp. nov. is proposed. The type strain is JS1^T (= DSM 27374^T = LMG 27616^T =JCM19211^T).</p> </div

International Journal of Pharma and Bio Sciences RESEARCH ARTICLE MICROBIOLOGY RESPONSE SURFACE METHODOLOGICAL OPTIMIZATION OF PRODUCTION CONDITION OF HYPERTHERMOSTABLE β AMYLASE FROM Bacillus subtilis DJ5 UNDER SOLID STATE FERMENTATION USING BARLEY AS SU

Eight different agro-residues were screened for production of hyperthermostable β amylase by Bacillus subtilis DJ5 using SSF. Barley showed highest production of 120.34 U/gdm after 41 hours of incubation. Three process parameters (initial moisture content, pH and incubation temperature) affecting enzyme production under SSF using barley as substrate were optimized with RSM. RSM experiments designed by ‘Stat Ease ’ software determined optimum conditions for maximum enzyme production. The best preferred physical conditions were 63.40 % initial moisture content, medium pH 6.947 and incubation temperature of 38.50 °C. Under such optimal condition, highest enzyme production of 130.62 U/gdm was recorded after 41 hours of incubation. Incubation temperature was found to play most crucial role for microbial growth and enzyme prodcution under SSF. This article can be downloaded from www.ijpbs.ne

Phytochemical screening and evaluation of cytotoxic and hypoglycemic properties of Mangifera indica peels

Objective: To investigate the presence of different phytoconstituents in Mangifera indica (M. indica) peel and evaluate its cytotoxicity to Artemia salina and hypoglycemic potential in Swiss albino mice. Methods: The methanolic extract of M. indica peel was used to determine the presence of phytoconstituents. Brine shrimp lethality bioassay method was followed to determine the cytotoxic potential of plant extract. In the case of hypoglycemic activity, oral administration of extract at 200 and 400 mg/kg and standard glibenclamide at 10 mg/kg was done, followed by determining the percentage of reduction of plasma glucose from the initial level. Results: The methanolic extract of M. indica peel showed the presence of flavonoid, saponin, steroid, tannins, terpenoids, glycosides and alkaloids. In brine shrimp lethality bioassay, the LC50 of the extract and standard vincristine sulfate was found to be 2.04 and 0.41 μg/mL, respectively. After 90 and 150 min, the methanolic extract at 200 and 400 mg/kg showed prominent plasma glucose reduction of 13.95%, 22.48% and 14.16%, 26.18% respectively compared to standard glibenclamide showing 14.90% and 20.67% plasma glucose reduction. Conclusions: This current research affirms prominent cytotoxic and moderate hypoglycemic potential of M. indica peel. Further bioactivity guided isolation of phytoconstituents and investigation on higher animals can lead to development of new drug molecules