NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis

The aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability, cost, and time required to obtain a result. Metabolomics may offer the potential to identify biomarkers that define calf sepsis in terms of combined clinical, physiological, and pathobiological abnormalities. To our knowledge, this is the first study presenting an NMR- (nuclear magnetic resonance-) based plasma metabolomics at set intervals in neonatal septic calves. Twenty neonatal dairy calves with severe sepsis and ten healthy calves were used. Hematological and biochemical health profiles were gathered in plasma samples at set intervals. Similarly, NMR spectra were acquired. All diseased animals (except one) died after 72 hours. Clinical and laboratory results were in accordance with those of severe septic animals. Multivariate analysis on NMR plasma spectra proved to be an excellent tool for faster identification of calves with severe sepsis from healthy animals. The NMR-based metabolomic profile may contribute to the better understanding of severe sepsis in newborn calves

NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis

The aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability, cost, and time required to obtain a result. Metabolomics may offer the potential to identify biomarkers that define calf sepsis in terms of combined clinical, physiological, and pathobiological abnormalities. To our knowledge, this is the first study presenting an NMR- (nuclear magnetic resonance-) based plasma metabolomics at set intervals in neonatal septic calves. Twenty neonatal dairy calves with severe sepsis and ten healthy calves were used. Hematological and biochemical health profiles were gathered in plasma samples at set intervals. Similarly, NMR spectra were acquired. All diseased animals (except one) died after 72 hours. Clinical and laboratory results were in accordance with those of severe septic animals. Multivariate analysis on NMR plasma spectra proved to be an excellent tool for faster identification of calves with severe sepsis from healthy animals. The NMR-based metabolomic profile may contribute to the better understanding of severe sepsis in newborn calves

NMR-based metabolomic evaluation in dairy cows with displaced abomasum

This study is the first to evaluate nuclear magnetic resonance (NMR) -based metabolomics in cows with displaced abomasum (DA), which is an internationally recognized problem in dairy cows. Some biochemical parameters have been used for monitoring DA. However, to date, few data have been available on the blood metabolomic profile of dairy cows. Forty Holstein multiparous cows with DA (30 left, 10 right) and 10 clinically healthy Holstein multiparous cows were the subjects of the study. All the animals had similar dry matter intake. An NMR-based metabolomics approach and hematological and biochemical analyses were performed. Some changes in biochemical parameters were observed between the groups. Among the cows with left displaced abomasum (LDA), 10 were associated with concomitant ketosis. Disease periods between the 2 DA groups were different. The metabolites identified and quantified by NMR analysis were valine, 3 beta-hydroxybutyrate (BHB), alanine, glutamine, glutamate, and succinate. The last of these was significantly decreased in cows with right displaced abomasum (RDA). Glutamine, glutamate, and 3 BHB levels were significantly different between DA groups. There was a positive correlation between BHB and valine, glutamine, and glutamate in the LDA group. Overall, this work suggests that the additional information obtained by NMR-based metabolomics evaluation may contribute to assessing the metabolic status of cows with DA.Scientific and Technological Research Council of Turkey (TUBITAK) [111O545]This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, project no: 111O545)

Total endoscopic left ventricle lipoma removal

Abstract Background Left ventricle (LV) lipoma is a very rare, benign cardiac tumor. Due to its rarity, LV lipoma is often misdiagnosed. Aspecific symptoms such as murmurs, arrhythmias, memory loss and palpitation may occur due to the mass effect. Case presentation We report a case report of a 42 year old woman who was found to have left ventricle mass after check-up for arrhytmia. By a fully endoscopic approach, the mass was successfully resected from the left ventricle without the need for sternotomy. Conclusion Total endoscopic removal of left ventricle lipoma’s can be done safely and has several advantages to conventional sternotomy. Larger studies are needed to confirm this hypothesis. </jats:sec

Total endoscopic left ventricle lipoma removal

BackgroundLeft ventricle (LV) lipoma is a very rare, benign cardiac tumor. Due to its rarity, LV lipoma is often misdiagnosed. Aspecific symptoms such as murmurs, arrhythmias, memory loss and palpitation may occur due to the mass effect.Case presentationWe report a case report of a 42 year old woman who was found to have left ventricle mass after check-up for arrhytmia. By a fully endoscopic approach, the mass was successfully resected from the left ventricle without the need for sternotomy.ConclusionTotal endoscopic removal of left ventricle lipoma's can be done safely and has several advantages to conventional sternotomy. Larger studies are needed to confirm this hypothesis.BackgroundLeft ventricle (LV) lipoma is a very rare, benign cardiac tumor. Due to its rarity, LV lipoma is often misdiagnosed. Aspecific symptoms such as murmurs, arrhythmias, memory loss and palpitation may occur due to the mass effect.Case presentationWe report a case report of a 42 year old woman who was found to have left ventricle mass after check-up for arrhytmia. By a fully endoscopic approach, the mass was successfully resected from the left ventricle without the need for sternotomy.ConclusionTotal endoscopic removal of left ventricle lipoma's can be done safely and has several advantages to conventional sternotomy. Larger studies are needed to confirm this hypothesis

Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

Background: Displaced abomasum (DA) is a condition of dairy cows that severely impacts animal welfare and causes huge economic losses. Objective: To assess the metabolic status of the disease using metabolomics in serum, urine and liver samples aimed at both water soluble and lipid soluble fractions. Methods: Fifty Holstein multiparous cows with DA (42 left, 8 right) and 20 clinically healthy Holstein multiparous cows were used. Left DA was associated with concomitant ketosis in 19 animals and right in two. NMR-based metabolomics approach and hematological and biochemical analyses were performed. Statistical analysis was carried out on H-1-NMR data after they have been normalized using PQN method. Results: Contrary to generated PCA score plots the OPLS-supervised method revealed differences between healthy animals and diseased ones based on serum water-soluble samples. While water and lipid soluble metabolites decreased in serum samples, fatty acid fractions and cholesterol were increased in liver samples in DA affected cows. The metabolomic and chemical profiles clearly revealed that cows with DA (especially with LDA) were at risk of ketosis and fatty liver. Serum hippuric acid concentration was significantly higher in healthy cows in comparison with LDA, whereas serum glycine concentration was reported higher for healthy when compared to RDA affected animals. Conclusion: A biochemical network and pathway mapping revealed 'valine, leucine and isoleucine biosynthesis' and 'phenylalanine, tyrosine and tryptophan biosynthesis' as the most probable altered metabolic pathway in DA condition. Serum was advocated as the optimal biological matrix for the H-1-NMR analysis.Selcuk University Scientific Research Projects Coordination UnitSelcuk University [17102035]; EC Contract INEXT [653706]This work was financially supported by the Selcuk University Scientific Research Projects Coordination Unit (Project No: 17102035). CERM/CIRMMP center of the ESFRI Instruct is gratefully acknowledged for the NMR access provision financially supported by the EC Contract INEXT No 653706

Plasma metabolomics in calves with acute bronchopneumonia

Background Bovine respiratory disease is one of the main health issues in dairy calves. Inflammatory lung diseases are highly complex with respect to pathogenesis and relationships between inflammation, clinical disease and response to treatment. Metabolomics may offer the potential to identify biomarkers that define calf bronchopneumonia in terms of combined clinical, physiological and patho-biological abnormalities. While metabolomic studies are often encountered in childhood pneumonia, there is no knowledge related to the same approach to calf pneumonia. Objective The aim of this first study was to reveal the new potential biomarkers for acute calf bronchopneumonia by single proton (H-1) Nuclear magnetic resonance (NMR) based quantitative metabolomics. Methods Fifty dairy calves with acute bronchopneumonia presented for treatment to the teaching hospital, and ten healthy dairy calves belonging the teaching farm were used. Laboratory (hematological: complete blood count and blood gas analysis, and biochemical analysis related to health profile) were performed. NMR spectra of the all samples (50 diseased + 10 healthy water soluble extracts, 50 diseased + 10 healthy lipid extracts) were acquired using a standard Nuclear Overhauser Effect Spectroscopy pulse sequence. Results NMR based metabolomics analysis showed that calves suffering from bronchopneumonia and healthy calves have two different and distinguishable metabolic fingerprints using both water soluble and lipid extracts. Alterations in metabolites, increases in 2-methyl glutarate, phenylalanine, phosphatidylcholine, and decreases in ethanol, dimethylsulfone, propionate, acetate, allantoin, free cholesterol, cholesterol (-C18), were meaningful for pathogenic mechanisms of calf bronchopneumonia. Conclusion The NMR based metabolomics may contribute to better understanding bronchopneumonia in calves.Selcuk University Scientific Research Projects Coordination UnitSelcuk University [15401135]; EC iNEXT [653706]; Fondazione VeronesiFondazione Umberto VeronesiThis work was financially supported by Selcuk University Scientific Research Projects Coordination Unit (Project No: 15401135). CERM/CIRMMP center of the ESFRI Instruct is gratefully acknowledged for the NMR access provision financially supported by the EC Contract iNEXT No 653706. This work was partially supported by Fondazione Veronesi that granted L. T through the Post-Doctoral Fellowship-2015