A STUDY ON THE CAUSES OF UNEMPLOYMENT AMONG UNIVERSITY GRADUATES IN KENYA: A CASE OF GARISSA COUNTY, KENYA

Unemployment is a major problem in Kenya. It has made many young university graduates demoralized. Unemployment rate has risen so high that in every 10 young people, close to 4 are jobless with requisite qualifications. Successive governments have done little to arrest the situation. This research was done to understand the causes of unemployment in Kenya and the solutions that can be put in place to mitigate the problem. The effects of unemployment and the relationship between creation of opportunities and the growth of economy. The research found out that unemployment in Kenya is very high. This shows lack of confidence they have the system in place. The main effects of unemployment are crime, corruption, nepotism and favourism, high dependency and drug abuse. Being a job creator rather than a job seeker is the major solution of unemployment in Kenya. The research also found out that aligning the education curriculum in line with the demand of the market is paramount and should be hastened. In conclusion, unemployment has caused a lot of problems in Kenya. The research recommended a raft of measures to reduce the issue of unemployment in the country. Encourage the youth to be job creators and not job seekers only. Universities should play an important role in this case. Universities should develop courses that are relevant and demand driven. Duplication of courses with fewer demands should be minimized as this will flood graduates with similar courses that are not needed at all. Technical education should be enhanced and proper mechanisms put in place to sponsor and encourage students to take up these courses. Strict regulations should be enacted to fight corruption, nepotism and favourism. Kenya needs a practical and proactive solution for this monster.  Article visualizations

SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum

Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist responsible for the most virulent form of human malaria, remain unknown. Here we present a thorough characterisation of PfTKL3 (PF13_0258), an enzyme that belongs to the tyrosine kinase-like kinase (TKL) group. We demonstrate by reverse genetics that PfTKL3 is essential for asexual parasite proliferation in human erythrocytes. PfTKL3 is expressed in both asexual and gametocytes stages, and in the latter the protein co-localises with cytoskeleton microtubules. Recombinant PfTKL3 displays in vitro autophosphorylation activity and is able to phosphorylate exogenous substrates, and both activities are dramatically dependent on the presence of an N-terminal "sterile α-motif” domain. This study identifies PfTKL3 as a validated drug target amenable to high-throughput screenin

Scorpions of the Horn of Africa (Arachnida, Scorpiones). Part XVII.Revision of \u3cem\u3eNeobuthus\u3c/em\u3e, with description of seven new species from Ethiopia, Kenya and Somaliland (Buthidae)

New information about the taxonomy and distribution of the genus Neobuthus Hirst, 1911 is presented, based on material recently collected mainly from Somaliland, but also Djibouti and Kenya. Emended diagnoses are proposed for N. berberensis Hirst, 1911 and N. ferrugineus (Kraepelin, 1898) sensu stricto. New species described are: N. amoudensis sp. n. from Ethiopia and Somaliland; N. erigavoensis sp. n., N. factorio sp. n., N. gubanensis sp. n., N. maidensis sp. n., and N. montanus sp. n. from Somaliland; and N. kloppersi sp. n. from Kenya. This doubles the number of species in Neobuthus, unearthing a rich diversification of this genus of diminutive buthids in the Horn of Africa. Included is a key and distribution map. In N. gubanensis sp. n., we report a second known case of anomalous pectine development with tarsal-like structures that implicate homeotic mutation, providing further evidence of pectine-leg homology in scorpions

Serological Conservation of Parasite-Infected Erythrocytes Predicts Plasmodium falciparum Erythrocyte Membrane Protein 1 Gene Expression but Not Severity of Childhood Malaria.

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), expressed on P. falciparum-infected erythrocytes, is a major family of clonally variant targets of naturally acquired immunity to malaria. Previous studies have demonstrated that in areas where malaria is endemic, antibodies to infected erythrocytes from children with severe malaria tend to be more seroprevalent than antibodies to infected erythrocytes from children with nonsevere malaria. These data have led to a working hypothesis that PfEMP1 variants associated with parasite virulence are relatively conserved in structure. However, the longevity of such serologically conserved variants in the parasite population is unknown. Here, using infected erythrocytes from recently sampled clinical P. falciparum samples, we measured serological conservation using pools of antibodies in sera that had been sampled 10 to 12 years earlier. The serological conservation of infected erythrocytes strongly correlated with the expression of specific PfEMP1 subsets previously found to be associated with severe malaria. However, we found no association between serological conservation per se and disease severity within these data. This contrasts with the simple hypothesis that P. falciparum isolates with a serologically conserved group of PfEMP1 variants cause severe malaria. The data are instead consistent with periodic turnover of the immunodominant epitopes of PfEMP1 associated with severe malaria.Wellcome Trust (084535, 077092, 084538, and 098635)This is the final version of the article. It first appeared from the American Society for Microbiology via http://dx.doi.org/10.1128/IAI.00772-1

The mRNA content of plasma extracellular vesicles provides a window into the brain during cerebral malaria disease progression

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/The impact of cerebral malaria on the transcriptional profiles of cerebral tissue is difficult to study using non-invasive approaches. We isolated plasma extracellular vesicles (EVs) from patients with cerebral malaria and community controls and sequenced their RNA content. Deconvolution of the tissue origins of the EV-RNA revealed that EVs from cerebral malaria patients are predominantly enriched in transcripts of brain origin. Next, we used manifold learning on the EV-RNAseq data to determine pseudotime against the community control samples as the baseline reference. We found that neuronal transcripts in plasma EVs decreased as pseudotime progressed, while transcripts of glial, endothelial, and immune cell origins increased over pseudotime. Pseudotime was associated with clinicopathological parameters of disease severity, including retinopathy, metabolic acidosis, respiratory rate, anaemia, malnutrition, depth of unconsciousness and death. Plasma EVs further provided evidence of platelet activation, TNF signalling, neurotrophin signalling, long-term potentiation and glutamatergic signalling during late disease stages of cerebral malaria. The transcriptional responses of cerebral tissue in cerebral malaria can be studied non-invasively using EVs circulating in peripheral blood.Non peer reviewe

Differential Plasmodium falciparum surface antigen expression among children with Malarial Retinopathy

Retinopathy provides a window into the underlying pathology of life-threatening malarial coma (“cerebral malaria”), allowing differentiation between 1) coma caused by sequestration of Plasmodium falciparum-infected erythrocytes in the brain and 2) coma with other underlying causes. Parasite sequestration in the brain is mediated by PfEMP1; a diverse parasite antigen that is inserted into the surface of infected erythrocytes and adheres to various host receptors. PfEMP1 sub-groups called “DC8” and “DC13” have been proposed to cause brain pathology through interactions with endothelial protein C receptor. To test this we profiled PfEMP1 gene expression in parasites from children with clinically defined cerebral malaria, who either had or did not have accompanying retinopathy. We found no evidence for an elevation of DC8 or DC13 PfEMP1 expression in children with retinopathy. However, the proportional expression of a broad subgroup of PfEMP1 called “group A” was elevated in retinopathy patients suggesting that these variants may play a role in the pathology of cerebral malaria. Interventions targeting group A PfEMP1 may be effective at reducing brain pathology

Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition

Malnutrition impacts approximately 50 million children worldwide and is linked to 45% of global mortality in children below the age of five. Severe acute malnutrition (SAM) is associated with intestinal barrier breakdown and epithelial atrophy. Extracellular vesicles including exosomes (EVs; 30–150 nm) can travel to distant target cells through biofluids including milk. Since milk-derived EVs are known to induce intestinal stem cell proliferation, this study aimed to examine their potential efficacy in improving malnutrition-induced atrophy of intestinal mucosa and barrier dysfunction. Mice were fed either a control (18%) or a low protein (1%) diet for 14 days to induce malnutrition. From day 10 to 14, they received either bovine milk EVs or control gavage and were sacrificed on day 15, 4 h after a Fluorescein Isothiocyanate (FITC) dose. Tissue and blood were collected for histological and epithelial barrier function analyses. Mice fed low protein diet developed intestinal villus atrophy and barrier dysfunction. Despite continued low protein diet feeding, milk EV treatment improved intestinal permeability, intestinal architecture and cellular proliferation. Our results suggest that EVs enriched from milk should be further explored as a valuable adjuvant therapy to standard clinical management of malnourished children with high risk of morbidity and mortality

Chloroquine resistance before and after its withdrawal in Kenya

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

From contigs towards chromosomes: automatic improvement of long read assemblies (ILRA)

Recent advances in long read technologies not only enable large consortia to aim to sequence all eukaryotes on Earth, but they also allow individual laboratories to sequence their species of interest with relatively low investment. Long read technologies embody the promise of overcoming scaffolding problems associated with repeats and low complexity sequences, but the number of contigs often far exceeds the number of chromosomes and they may contain many insertion and deletion errors around homopolymer tracts. To overcome these issues, we have implemented the ILRA pipeline to correct long read-based assemblies. Contigs are first reordered, renamed, merged, circularized, or filtered if erroneous or contaminated. Illumina short reads are used subsequently to correct homopolymer errors. We successfully tested our approach by improving the genome sequences of Homo sapiens, Trypanosoma brucei, and Leptosphaeria spp., and by generating four novel Plasmodium falciparum assemblies from field samples. We found that correcting homopolymer tracts reduced the number of genes incorrectly annotated as pseudogenes, but an iterative approach seems to be required to correct more sequencing errors. In summary, we describe and benchmark the performance of our new tool, which improved the quality of novel long read assemblies up to 1 Gbp. The pipeline is available at GitHub: https://github.com/ThomasDOtto/ILRA

Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum

© 2023 The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extracellular vesicles is not only periodic throughout the asexual intraerythrocytic developmental cycle but is also highly conserved across P. falciparum isolates. We further demonstrate that the phases of RNA secreted via extracellular vesicles are discernibly shifted compared to those of the intracellular RNA within the secreting whole parasite. Finally, transcripts of genes with no known function during the asexual intraerythrocytic developmental cycle are enriched in PfEVs compared to the whole parasite. We conclude that the secretion of extracellular vesicles could be a putative posttranscriptional RNA regulation mechanism that is part of or synergise the classic RNA decay processes to maintain intracellular RNA levels in P. falciparum.Peer reviewe