27 research outputs found
Analysis of the Origin of Emiratis as Inferred from a Family Study Based on HLA-A, -C, -B, -DRB1, and -DQB1 Genes
In this study, we investigated HLA class I and class II allele and haplotype frequencies in Emiratis and compared them to those of Asian, Mediterranean, and Sub-Saharan African populations. Methods: Two-hundred unrelated Emirati parents of patients selected for bone marrow transplantation were genotyped for HLA class I (A, B, C) and class II (DRB1, DQB1) genes using reverse sequence specific oligonucleotide bead-based multiplexing. HLA haplotypes were assigned with certainty by segregation (pedigree) analysis, and haplotype frequencies were obtained by direct counting. HLA class I and class II frequencies in Emiratis were compared to data from other populations using standard genetic distances (SGD), Neighbor-Joining (NJ) phylogenetic dendrograms, and correspondence analysis. Results: The studied HLA loci were in Hardy-Weinberg Equilibrium. We identified 17 HLA-A, 28 HLA-B, 14 HLA-C, 13 HLA-DRB1, and 5 HLA-DQB1 alleles, of which HLA-A*02 (22.2%), -B*51 (19.5%), -C*07 (20.0%), -DRB1*03 (22.2%), and -DQB1*02 (32.8%) were the most frequent allele lineages. DRB1*03~DQB1*02 (21.2%), DRB1*16~DQB1*05 (17.3%), B*35~C*04 (11.7%), B*08~DRB1*03 (9.7%), A*02~B*51 (7.5%), and A*26~C*07~B*08~DRB1*03~DQB1*02 (4.2%) were the most frequent two- and five-locus HLA haplotypes. Correspondence analysis and dendrograms showed that Emiratis were clustered with the Arabian Peninsula populations (Saudis, Omanis and Kuwaitis), West Mediterranean populations (North Africans, Iberians) and Pakistanis, but were distant from East Mediterranean (Turks, Albanians, Greek), Levantine (Syrians, Palestinians, Lebanese), Iranian, Iraqi Kurdish, and Sub-Saharan populations. Conclusions: Emiratis were closely related to Arabian Peninsula populations, West Mediterranean populations and Pakistanis. However, the contribution of East Mediterranean, Levantine Arab, Iranian, and Sub-Saharan populations to the Emiratis' gene pool appears to be minor.This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors in the UAE. This work was supported by the National Institutes of Health (NIH) and National Institute of Allergy and Infectious Disease (NIAID), grant R01AI128775 (SJM). The content is solely the responsibility of the authors and does not necessarily reflect the official views of the NIAID, NIH, and United States government.Scopu
Association of HLA Class II Alleles and Haplotypes with Type 1 Diabetes in Tunisian Arabs
HLA Class I and Class II two-Locus haplotypes in Tunisians.
<p>HLA Class I and Class II two-Locus haplotypes in Tunisians.</p
The closest populations to Arabs using standard genetic distances (SGD) based on <i>HLA-DRB1*</i> alleles.
<p>The closest populations to Arabs using standard genetic distances (SGD) based on <i>HLA-DRB1*</i> alleles.</p
Correspondence analysis showing a global view of the relationship among Mediterranean populations according to HLA allele frequencies in three dimensions (bi-dimensional representation).
<p>HLA-DRB1 allele frequencies data. Only individuals with defined DRB1 subtypes are considered.</p
Most frequent <i>HLA-A*</i> and–<i>B*</i> alleles in Arab populations.
<p>Most frequent <i>HLA-A*</i> and–<i>B*</i> alleles in Arab populations.</p
The most frequent (%) <i>HLA</i> extended haplotypes in Arabs.
<p>The most frequent (%) <i>HLA</i> extended haplotypes in Arabs.</p
Most frequent <i>HLA-DRB1*</i> and–<i>DQB1*</i> alleles in Arab populations.
<p>Most frequent <i>HLA-DRB1*</i> and–<i>DQB1*</i> alleles in Arab populations.</p
Neighbor-Joining dendrogram showing relatedness between Tunisians and other populations.
<p>Standard genetic distances (SGD) between populations were calculated by using generic HLA-DRB1 and -DQB1 genotyping. Data from other populations were taken from references detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136909#pone.0136909.t001" target="_blank">Table 1</a>. Bootstrap values from 1.000 replicates are shown.</p
Standard genetic distances (SGD<sup>1</sup>) between Tunisians and other populations.
<p><sup>1.</sup> Obtained by using HLA-DRB1 allele frequencies.</p><p><sup>2.</sup> Refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136909#pone.0136909.t001" target="_blank">Table 1</a> for profile of populations studied.</p><p>Standard genetic distances (SGD<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136909#t003fn001" target="_blank"><sup>1</sup></a>) between Tunisians and other populations.</p