Stachybotrys chartarum: A Novel Biological Agent for The Extracellular Synthesis of Silver Nanoparticles and Their Antimicrobial Activity

Microbial assisted biosynthesis of nanoparticles is a rapidly progressing area of nanobiotechnology. Inthis paper Stachybotrys chartarum assisted extracellular synthesis of silver nanoparticles (AgNPs) is reportedwhen challenged with 1mM silver nitrate (AgNO3). The characterization of AgNPs was carried out visualobservation and UV-Vis spectrophotometry. Further analysis carried out by Fourier Transform InfraredSpectroscopy (FTIR), provides evidence for the presence of proteins as capping agent, which helps in increasingthe stability of the synthesized AgNPs. Transmission Electron Microscopy (TEM) investigations confi rmedthat AgNPs were formed. The synthesized silver nanoparticles were found in the range of 65-108 nm. Finally,the antimicrobial susceptibility of AgNPs synthesized was investigated which exhibited more potent activityagainst bacteria than fungi compared with using silver nitrate at concentration 1mM. Keywords: Antimicrobial activity, Stachybotrys chartarum, Silver nanoparticle

Stachybotrys chartarum: A Novel Biological Agent for The Extracellular Synthesis of Silver Nanoparticles and Their Antimicrobial Activity

Microbial assisted biosynthesis of nanoparticles is a rapidly progressing area of nanobiotechnology. Inthis paper Stachybotrys chartarum assisted extracellular synthesis of silver nanoparticles (AgNPs) is reportedwhen challenged with 1mM silver nitrate (AgNO3). The characterization of AgNPs was carried out visualobservation and UV-Vis spectrophotometry. Further analysis carried out by Fourier Transform InfraredSpectroscopy (FTIR), provides evidence for the presence of proteins as capping agent, which helps in increasingthe stability of the synthesized AgNPs. Transmission Electron Microscopy (TEM) investigations confi rmedthat AgNPs were formed. The synthesized silver nanoparticles were found in the range of 65-108 nm. Finally,the antimicrobial susceptibility of AgNPs synthesized was investigated which exhibited more potent activityagainst bacteria than fungi compared with using silver nitrate at concentration 1mM.Keywords: Antimicrobial activity, Stachybotrys chartarum, Silver nanoparticles</div

ASSESSING THE BIOACTIVITY AND ANTIOXIDATIVE PROPERTIES OF SOME COMPOUNDS ISOLATED FROM ABUTILON HIRTUM (LAM.)

ABSTRACTObjectives: The present study on the methanol extract of Abutilon hirtum (Lam.) [Malvaceae] afforded ten compounds. Findings from this assessmentindicated that A. hirtum leaves possessed vast potential as medicinal product especially in liver cancer treatment.Methods: Dried-powdered leaves were boiled under reflux in 10 L of petroleum ether for 8 hrs. After filtration, the solvent was evaporated; afforded15 g of petroleum ether extract followed by boiling with reflux for 8 hrs with chloroform, then filtration and the residue was evaporated to give 34 gchloroform extract. Ethyl acetate was added and refluxed for 8 hrs, then filtration and evaporation to give 31 g ethyl acetate extract. Finally the leaveswere refluxed with 10 L of 85 % aqueous MeOH, after cooling, the solution was filtered and evaporated and the residue was 210 g methanol extractthen the residue was dissolved in de-ionized water (250 ml), then the salt was removed by adding excess methanol solution (2.5 L), and finally filtered.The cytotoxicity of the isolated compounds was evaluated towards HepG2 liver-carcinoma cell line using MTT assay, the antimicrobial activity wastested using the Disc agar plate method and the total antioxidant capacity was determined according to phosphomolybdenum method.Results: The isolated compounds identified as methyl gallate, cuneataside E, bergapten, gallic acid, ellagic acid, epigallocatechin-3-O-gallate,kaempferol-3-O-α-L-rhamnoside, benzyl-1-O-β-D-glucopyranoside, 2(4-hydroxyphenyl)ethyl-O-β-D-glucopyranoside and β-sitosterol. All the isolatedcompounds are known; but they were isolated from Abutilon hirtum for the first time.Conclusion: This report may serve as a footprint concerning the biological and pharmacological activities of A. hirtum leaves.Keywords: Abutilon hirtum, Malvaceae, HepG2, Phenolics, Antioxidant.Â

Effect of Time of Interradilarcular Preparation on Apical Microleakage of Endodontically Treated Teeth

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Correlation between Colour Duplex and Technetium-99m Thyroid Scan in Identifying the Cause of Toxic Goitre

Introduction: Differentiation between thyroiditis induced thyrotoxicosis and Graves' disease is important for selection of proper therapy. It is often difficult to make this distinction without performing thyroid scintigraphy. Color flow Doppler sonography (CFDS) is gaining importance for the functional evaluation of the thyroid disorders. We aimed to determine the value of CFDS for the etiological diagnosis of thyrotoxicosis.Patients and methods: Thirty patients with clinical and lab signs of hyperthyroidism (12 Graves’ disease and, 18 patients with Hashimoto’s thyroiditis) with 10 euthyroid controls were evaluated.Etiological diagnosis was carried out using standard methods. Conventional grey scale sonography was done, followed by CFDS. Doppler patterns of the glands were scored and peak systolic velocity (PSV) measurements were obtained from superior and inferior thyroid arteries. Results were correlated to isotope scanning which was used as a golden standard.Results: Vascular patterns were significantly more prominent, and the mean PSV values were significantly higher in the Graves' Disease patients compared to the thyroiditis patients than controls. CFDS could differentiate the untreated Graves' Disease from the thyroiditis which had similar grey scale findings but higher vascular patterns and significantly higher PSV values, sensitivity = 96.6%, specificity= 96.6 %, and P&gt;0.001Conclusion: As an inexpensive, fast, and noninvasive imaging procedure, CFDS could be helpful in the initial clinical evaluation and may avoid scintigraphy in a substantial number of thyrotoxic patients

Correlation between Colour Duplex and Technetium-99m Thyroid Scan in Identifying the Cause of Toxic Goitre

Introduction: Differentiation between thyroiditis induced thyrotoxicosis and Graves' disease is important for selection of proper therapy. It is often difficult to make this distinction without performing thyroid scintigraphy. Color flow Doppler sonography (CFDS) is gaining importance for the functional evaluation of the thyroid disorders. We aimed to determine the value of CFDS for the etiological diagnosis of thyrotoxicosis. Patients and methods: Thirty patients with clinical and lab signs of hyperthyroidism (12 Graves’ disease and, 18 patients with Hashimoto’s thyroiditis) with 10 euthyroid controls were evaluated.Etiological diagnosis was carried out using standard methods. Conventional grey scale sonography was done, followed by CFDS. Doppler patterns of the glands were scored and peak systolic velocity (PSV) measurements were obtained from superior and inferior thyroid arteries. Results were correlated to isotope scanning which was used as a golden standard.  Results: Vascular patterns were significantly more prominent, and the mean PSV values were significantly higher in the Graves' Disease patients compared to the thyroiditis patients than controls. CFDS could differentiate the untreated Graves' Disease from the thyroiditis which had similar grey scale findings but higher vascular patterns and significantly higher PSV values, sensitivity = 96.6%, specificity= 96.6 %, and P&gt;0.001 Conclusion: As an inexpensive, fast, and noninvasive imaging procedure, CFDS could be helpful in the initial clinical evaluation and may avoid scintigraphy in a substantial number of thyrotoxic patients

Predictive Modeling of Multilayer Graphene Growth by Chemical Vapour Deposition on Co-Ni/Al2O3 Substrate using Artificial Neural Network

The uniqueness of multilayer graphene as extremely high carrier mobility, tune-able band gap and high elasticity has made it be considered as a high prospect engineering material that can be employed for several applications such as solar cells, field effect transistors, super-capacitors, batteries and sensors. In this study, the application of Artificial Neural Networks (ANN) for the predictive modeling of multilayer graphene (MLG) growth by chemical vapor deposition (CVD) on Co-Ni/Al2O3 substrate was investigated. Data comprises temperature, catalyst compositions, ethanol flowrates were generated using central composite experimental design and employed to obtain the MLG yield as the response. The data were subsequently used for predictive modeling using ANN. The findings show that the predictive values of the MLG yields were in good agreement with those obtained from the experimental runs having a coefficient of determination (R2 ) of 0.988

Energy and water saving by using modified closed circuits of drip irrigation system

The aim of this research was determine the energy and water use efficiencies under the modification of closed circuit drip irrigation systems designs. Field experiments carried out on transgenic maize (GDH, LL3), (Zea Mays crop) under two types of closed circuits: a) One manifold for lateral lines or Closed circuits with One Manifold of Drip Irrigation System (CM1DIS); b) Closed circuits with Two Manifolds of Drip Irrigation System (CM2DIS), and c) Traditional Drip Irrigation System (TDIS) as a control. Three lengths of lateral lines were used, 40, 60, and 80 meters. PE tubes lateral lines: 16 mm diameter; 30 cm emitters distance, and GR built-in emitters 4 lph when operating pressure 1 bar under Two levels slope conditions 0% and 2%. Experiments were conducted at the Agric. Res. Fields., Soil and Plant & Agric. System Dept., Agric. Faculty, Southern Illinois University, Carbondale (SIUC), Illinois, USA. Under 0% level slope when using CM2DIS the increase percent of Energy Use Efficiency (EUE) were 32.27, 33.21, and 34.37% whereas with CM1DIS were 30.84, 28.96, and 27.45% On the other hand when level slope 2% were with CM2DIS 31.57, 33.14, and 34.25 while CM1DIS were 30.15, 28.98, and 27.53 under lateral lengths 40, 60 and 80 m respectively relative to TDIS. Water Use Efficiency (WUE) when level slope 0% under CM2DIS were 1.67, 1.18, and 0.87 kg/m3 compared to 1.65, 1.16, and 0.86 kg/m3 with CM1DIS and 1.35, 1.04, and 0.75 kg/m3 with TDIS whereas with level slope 2% when using CM2DIS were 1.76, 1.29, and 0.84 kg/m3 compared to 1.77, 1.30, and 0.87 kg/m3 with CM1DIS and 1.41, 1.12, and 0.76 kg/m3 (for lateral lengths 40, 60, and 80 meters respectively). Water saving percent varied widely within individual lateral lengths and between circuit types relative to TDIS. Under slope 0% level CM2DIS water saving percent values were 19.26, 12.48, and 14.03%; with CM1DIS they were 18.51, 10.50, and 12.78%; and under slope level 2% with CM2DIS they were 19.93, 13.26, and 10.38% and CM1DIS were 20.49, 13.96, and 13.23% (for lateral lengths 40, 60, 80 meters respectively). The energy use efficiency and water saving were observed under CM2DIS and CM1DIS when using the shortest lateral length 40 meters, then lateral length 60 meters, while the lowest value was observed when using lateral length 80 meters this result depends on the physical and hydraulic characteristics of the emitters, lateral line uniformity, and friction losses. CM2DIS was more energy use efficiency, EUE, water saving, and WUE than either CM1DIS or TDIS