Evaluation of Allelopathic Potential of Rumex dentatus Root Extract and Allelochemicals on Cicer arietinum

The present study aimed to evaluate the allelopathic potential of root extract of Rumex dentatus L. and various allelochemicals on some physiological parameters in roots of Cicer arietinum L. The tested allelochemicals were benzoic, caffeic, cinnamic, ferulic, gallic, sinapic and vanillic acids. Seed germination of Cicer was inhibited by Rumex extract. Lipid peroxidation and hydrogen peroxide production increased gradually in response to extract concentration as well as allelochemicals treatment. The total soluble protein decreased whereas the total phenol increased under the various treatments. The activities of superoxide dismutase (SOD: 1.15.1.1) and catalase (CAT: 1.11.1.6) increased continuously with increasing extract concentration. However, peroxidase (POD: 1.11.1.7) increased sharply at 2% (w/v) followed by reduction at the higher concentrations and reached 1.1 U g-1 fresh weight at 10% (w/v) which was still higher than that of the control value. Ferulic acid was the most effective inducer for SOD activity followed by cinnamic acid. The POD activity increased remarkably particularly with cinnamic, benzoic and gallic acids. Sinapic, ferulic and coumaric acids enhanced CAT activity by 150.4%, 139.5% and 124.4%, respectively. The results reveal the possible use of R. dentatus as bioherbicide

Osteopontin for Early Detection of Microvascular and Macrovascular Type 1 Diabetic Complication

AIM: To evaluate the relationship between osteopontin and diabetes complication in type 1 diabetic patient. PATIENTS AND METHODS: Seventy types 1 diabetic and 60 healthy volunteers were studied. Full history, examination, laboratory tests of glycosylated haemoglobin (HbA1c), serum lipids {cholesterol, triglyceride (Tg), high-density lipoprotein-cholesterol (HDL-c), low-density lipoprotein – cholesterol (LDL-c)}, oxidised low-density lipoprotein (OxLDL), Osteopontin and urinary microalbuminuria (albumin/creatinine ratio) were done. Image study in the form of a carotid intimal medial thickness (cIMT) and aortic intimal medial thickness (aIMT), renal doppler for resistivity index was also done for all participant included in the study. RESULTS: Urinary albumin/creatinine ratio, lipid profile, osteopontin, cIMT and aIMT were higher in people with diabetes. Osteopontin was higher in people with diabetes with positive microalbuminuria and cIMT. Systolic blood pressure, microalbuminuria and cIMT had a positive correlation with osteopontin in people with diabetes. Stepwise multiple regression analysis showed that osteopontin had a significant correlation with cIMT. Receiver operating characteristic (ROC) curve showed that the cut off value of Osteopontin for detection of cIMT was > 60 with a specificity of 100% and sensitivity 80.5%, while that of albumin/creatinine ratio was > 64 with a specificity of 66.7 and sensitivity of 92.3. CONCLUSION: Osteopontin is higher in type 1 diabetics and is useful for early detection of diabetic microvascular and macrovascular complication

Optimization of Irrigation and Leaching Depths Considering the Cost of Water Using WASH_1D/2D Models

Optimization of water use with consideration of salinity control is a crucial task for crop production. A new scheme, “optimized irrigation”, was recently presented to determine irrigation depth using WASH_1D/2D which are numerical simulation models of water flow and solute transport in soils and crop growth. In the scheme, irrigation depth is determined such that net income is maximized considering the price of water and weather forecasts. To evaluate whether the optimized irrigation is also able to restrict salinity stress and avoid salinization without any intentional leaching, we carried out a numerical experiment for winter wheat grown in northern Sudan under the following scenarios: (1) Available water in the root zone is refilled using freshwater (0.17 g/L of NaCl) at every five days; (2) available water in the root zone is refilled using saline water (1.7 g/L) at every five days; (3) optimized irrigation using fresh water at 7-days interval; (4) optimized irrigation on a weekly basis using saline water; and (5) same as scenario 2, except for leaching is carried out at the middle of the growing season and leaching depth is optimized such that net income is maximized. The results showed that the optimized irrigation scheme automatically instructs additional water required for leaching at each irrigation event and maximizes the net income even under saline conditions

Three Dimentional Transvaginal U/S Measurements Of Uterine Junctional Zone Thickness And Its Relation To Implantation Success Rate In ICSI Cycles

Background: Sub-endometrial junction zone (JZ) plays an important role in most of reproductive functions. Objective: To find out the effect of the sub-endometrial JZ thickness assessment by 3-D transvaginal ultrasound (TVUS) on intracytoplasmic sperm injection (ICSI) outcomes in patients with unexplained recurrent implantation failure (RIF). Setting: ART Unit of Obstetrics and Gynecology Department, Faculty of Medicine, South Valley University, Qena, Egypt. Duration: From April 2016 to October 2018. Study Design: A prospective observational study. Methds: Fifty couples with history of unexplained RIF in previous ICSI cycles and prepared for another ICSI cycle (group I) and fifty couples with unexplained infertility prepared for ICSI for the first time (group II) had been included in this study. At time of ovum pick up, 3-DTVUS was done for all cases in both groups for assessment of sub endometrial junction zone thickness and correlated with ICSI outcome. Results:There were statistically significant differences be- tween group I and group II in JZ thickness in the 3 uterine regions (fundus, anterior and posterior walls) with p < 0.001, but there were mildly statistically significant differences between both groups in chemical and clinical preg- nancy rates with p < 0.01. Conclusions: The thickness of JZ in patients with history of unexplained RIF was higher than those with unexplained infertility scheduled for ICSI. The JZ thickness was inversely correlated with increased embryo implantation rates in ICSI procedures, the thinner the JZ thickness was associated with higher pregnancy rates (both chemical and clinical pregnancy rates)

The safe re-use of wastewater for agriculture is a desired goal in many arid zone countries. The potential of greywater as alternative irrigation source for vegetable crops was investigated. To-mato, pea and cantaloupe plants were drip irri-gated with both fresh Nile water and greywater to access the impact on yield production and asso-ciated environmental and health risks. The biolog-ical properties of the two different sources of wa-ter clearly indicated that greywater was extremely higher in

The safe re-use of wastewater for agriculture is a desired goal in many arid zone countries. The potential of greywater as alternative irrigation source for vegetable crops was investigated. Tomato, pea and cantaloupe plants were drip irrigated with both fresh Nile water and greywater to access the impact on yield production and associated environmental and health risks. The biological properties of the two different sources of water clearly indicated that greywater was extremely higher in bacterial content compared with fresh Nile water. Pea plants showed significantly higher yield irrigated with fresh Nile water, however, tomato and cantaloupe plants gave significantly higher yield irrigated with greywater. Generally, the coliform populations in untreated greywater irrigated plants were higher than those irrigated with Nile water in all tested vegetables. The percentages of increasing in total coliform in untreated-irrigated greywater vegetables were 27.95%, 34.55% and 41.4% for pea, tomato and cantaloupe (averaged over outer and inner fruit tissues), respectively. Unexpectedly, central part of fruits for pea and tomato had highest coliform counts when compared to the outer surface using both Nile and untreated greywater. Overall, irrigation with greywater increased soil bacterial content by 15% while fresh Nile water increased it by 13% at the end of the experiment. In addition, greywater elevated the content of soil total coliform by 52% where fresh Nile water increased it by 30%. The results of this study indicated that untreated greywater should not consider as an alternative irrigation source for edible crops such as vegetables. In current investigation, the beneficial effects in tomato through giving significantly higher yield with greywater became worthless after the enormous fecal contamination that was detected in fruits. Several considerations must be adopted to minimize the health and environmental risks associated with greywater reuse in irrigation of vegetable crops

Arabic gum acacia improves diabetic peripheral neuropathy in rats: a biochemical and histopathological evidence

Background: Diabetic peripheral neuropathy (DPN) is a frequent complication of diabetes mellitus and unfortunately, its present therapeutic alternatives are exceptionally poor. Objectives of this study was to assess the antidiabetic, antioxidant and hypolipidemic action of Gum Arabic (GA) and its role in promoting the functional recovery from diabetic neuropathy developed in in an experimental model of diabetic neuropathy.Methods: Sixty adult male Sprague-Dawley rats were utilized and randomly assigned into six groups (n= 10); control, Arabic gum-treated, untreated diabetic, diabetic received metformin, diabetic received metformin and B12 vitamin and diabetic received metformin, B12 vitamin and AG. Locomotor activity and hyperalgesia were assed at the end of the study. Fasting and two hours post-prandial blood glucose, serum insulin levels, lipid Profile, oxidants/antioxidants parameters were assessed in the blood. Sciatic nerve was assessed histopathologically.Results: The locomotor activity of the untreated diabetic rats was significantly (p<0.001) reduced compared to the control group while it was significantly increased in all treated groups. The lipid profile and Malondialdehyde were significantly improved in all treated groups. Levels of CAT, GSH, SOD, GPx were significantly decreased in untreated diabetic group compared to the control while they were significantly increased in all treated groups compared to the untreated diabetic group. Sciatic nerve fibers of untreated diabetic rats showed degenerated axons with dilated myelin sheaths and degenerated Schwann cells. The nerve had significantly fewer fiber compared to the control. These changes were alleviated in all the treated groups specifically that received metformin, vitamin B12 and GA.Conclusions: It could be concluded that Arabic gum had hypoglycemic, antioxidant and hypolipidemic activity and had a protective effect on diabetic neuropathy. Based on this it is recommended that human clinical trials are necessary to prove this therapeutic effect

EFFECT OF DIFFERENT ALKALI CONCENTRATIONS DURING THE REFINING PROCESS ON THE OXIDATIVE STABILITY OF SUNFLOWER AND SOYBEAN OILS

The oxidative stability of bleached soybean and sunflower oils which were previously refined using NaOH and KOH at concentrations of 12, 14 and 16 Béo was studied in comparison with semirefined sunflower and crude soybean oils. Semirefined sunflower oil and crude soybean oil showed the highest stability. Oils previously refined using 12 Béo NaOH or KOH had an acceptable stability. Higher NaOH concentrations resulted in a decrease of stability

EFFECT OF DIFFERENT ALKALI CONCENTRATIONS DURING THE REFINING PROCESS ON THE FATTY ACID COMPOSITION OF SUNFLOWER AND SOYBEAN OILS

The effect of NaOH and KOH at concentrations of 12, 14 and 16 Bé° during the refining of sunflower and soybean oils on the fatty acid composition was studied using GLC technique. The quantity of linolenic, linoleic, oleic, palmitic and stearic acid as well as the USFA (unsaturated fatty acids): SFA (saturated fatty acids) ratio were measured. Only slight changes in the quantity of individual fatty acids were found particularly at higher alkali concentrations. The changes in the USFA : SFA ratio compared with crude or semirefined oil were not significant

Nitric Oxide Gene Polymorphism is a Risk Factor for Diabetic Nephropathy and Atherosclerosis in Type 1 Diabetic Patients

AIM: To assess the risk factor for diabetic atherosclerosis nephropathy and diabetic nephropathy in type 1 diabetic patients. PATIENTS AND METHODS: Thirty healthy volunteers age and sex-matched and Sixty-five type 1 diabetic patient were in rolled in the study. The mean age of patients was 17.99 ± 2.59 years, mean age of onset of diabetes was 7.00 ± 3.28 years, mean duration of diabetes was 10.91 ± 3.54 years. Glycosylated sex-matched (HbA1c) was assessed in blood samples, serum lipid profile was determined, and serum level of oxidised low-density lipoprotein (OxLDL), and nitric oxide was evaluated by enzyme-linked immunosorbent assay (ELISA) technique. Nitric oxide 894G &gt; T genotype was analysed by (PCR-RFLP) method and confirmed by Sequencing. Assessment of the albumin / creatinine ratio was done in urine samples. Renal Doppler and Carotid intima-media thickness (cIMT) via ultrasound was also performed. RESULTS: OxLDL, lipid profile, albumin/creatinine ratio, cIMT and resistivity index were significantly higher in diabetic patients while nitric oxide was significantly lower. Nitric oxide genotype shows no significant difference between diabetic’s patients and controls. Diabetic patients with homozygous NO had a significantly lower serum level of Nitric oxide, a significantly higher OxLDL, albumin / creatinine ratio and lipid profile. CONCLUSION: diabetic patients are liable for the occurrence of early diabetic nephropathy and atherosclerosis as a result of the presence of low level of nitric oxide. Nitric oxide gene polymorphism 894G &gt; T in diabetic patients is a risk factor for diabetic nephropathy and atherosclerosis

EFFECT OF REFINING ON THE PHYSICAL AND CHEMICAL PROPERTIES OF SUNFLOWER AND SOYBEAN OILS

The effect of different refining processes on the physical (colour, specific gravity, refractive index) and chemical (acidity, saponification number, ester number, iodine value, peroxide value, unsaponifiable matter) properties of sunflower and soybean oils was studied. Degumming and dewaxing procedures caused negligible differences in the properties mentioned above. Treatment with NaOH or KOH of 12, 14 and 16 Be⁰ concentration resulted in a reasonable refining loss and residual acidity. Using Na₂C0₃ and NaHC0₃ or KOH and NaOH of higher concentration (over 16 Be⁰) caused high refining losses and unacceptable residual acidity