An evaluation of teachers and students in high schools: knowledge of first aid and resuscitation

The aim of this study was twofold. Firstly, to investigate the number of teachers with Resuscitation training and secondly, to identify how many students are receiving Resuscitation instruction while at school. Presently, there is no uniformity in the method of First Aid and Resuscitation instruction for students in New South Wales High Schools. Current epidemiological data suggest that the selected region examined in this study is representative of the state of New South Wales. The author posits Resuscitation skills as an essential part of schooling and daily living. The teacher/student relationship is such that the teacher is under a duty of care requiring, among other things, that the teacher knows Resuscitation skills in case of an emergency

The Reverend Jim Jones and Religious, Political, and Racial Radicalism in Peoples Temple

On November 18, 1978 over 900 members of Peoples Temple committed suicide or were murdered in Jonestown, Guyana under the direction of Reverend Jim Jones. This thesis explores the radical ideology of Jones leading up to and including the day of the murder-suicides by poisoned Flavor-Aid. Jones was a radical theologically, politically, and in racial thinking, although he was not an advocate for women’s rights. Jones claimed to be a prophet and then God, criticized the Bible and became atheistic, called himself a Marxist, a socialist, and a Communist, and strove for equal rights for minorities in the United States through his interactions with the Black Panther Party and prominent black religious figures, including Father Divine. The “cult” was said to have committed “revolutionary suicide,” a phrase used by Huey Newton. Jones’ radicalism in Peoples Temple may be one factor that ultimately led to the destruction of Jonestown that day

Dipeptidyl Peptidase 10 (DPP10789): A Voltage Gated Potassium Channel Associated Protein Is Abnormally Expressed in Alzheimer’s and Other Neurodegenerative Diseases

Copyright © 2014 Tong Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.The neuropathological features associated with Alzheimer’s disease (AD) include the presence of extracellular amyloid- peptidecontaining plaques and intracellular tau positive neurofibrillary tangles and the loss of synapses and neurons in defined regions of the brain. Dipeptidyl peptidase 10 (DPP10) is a protein that facilitates Kv4 channel surface expression and neuronal excitability. This study aims to explore DPP10789 protein distribution in human brains and its contribution to the neurofibrillary pathology of AD and other tauopathies. Immunohistochemical analysis revealed predominant neuronal staining of DPP10789 in control brains, and the CA1 region of the hippocampus contained strong reactivity in the distal dendrites of the pyramidal cells. In AD brains, robust DPP10789 reactivity was detected in neurofibrillary tangles and plaque-associated dystrophic neurites, most of which colocalized with the doubly phosphorylated Ser-202/Thr-205 tau epitope. DPP10789 positive neurofibrillary tangles and plaque-associated dystrophic neurites also appeared in other neurodegenerative diseases such as frontotemporal lobar degeneration, diffuse Lewy body disease, and progressive supranuclear palsy. Occasional DPP10789 positive neurofibrillary tangles and neurites were seen in some aged control brains.Western blot analysis showed both full length and truncatedDPP10789 fragments with the later increasing significantly in AD brains compared to control brains. Our results suggest that DPP10789 is involved in the pathology of AD and other neurodegenerative diseases

Bioactivity of the Murex Homeopathic Remedy and of Extracts from an Australian Muricid Mollusc against Human Cancer Cells

Marine molluscs from the family Muricidae are the source of a homeopathic remedy Murex, which is used to treat a range of conditions, including cancer. The aim of this study was to evaluate the in vitro bioactivity of egg mass extracts of the Australian muricid Dicathais orbita, in comparison to the Murex remedy, against human carcinoma and lymphoma cells. Liquid chromatography coupled with mass spectrometry (LC-MS) was used to characterize the chemical composition of the extracts and homeopathic remedy, focusing on biologically active brominated indoles. The MTS (tetrazolium salt) colorimetric assay was used to determine effects on cell viability, while necrosis and apoptosis induction were investigated using flow cytometry (propidium iodide and Annexin-V staining, resp.). Cells were treated with varying concentrations (1–0.01 mg/mL) of crude and semi-purified extracts or preparations (dilute 1 M and concentrated 4 mg/mL) from the Murex remedy (4 h). The Murex remedy showed little biological activity against the majority of cell lines tested. In contrast, the D. orbita egg extracts significantly decreased cell viability in the majority of carcinoma cell lines. Flow cytometry revealed these extracts induce necrosis in HT29 colorectal cancer cells, whereas apoptosis was induced in Jurkat cells. These findings highlight the biomedical potential of Muricidae extracts in the development of a natural therapy for the treatment of neoplastic tumors and lymphomas

Bilateral enucleation alters gene expression and intraneocortical connections in the mouse

<p>Abstract</p> <p>Background</p> <p>Anatomically and functionally distinct sensory and motor neocortical areas form during mammalian development through a process called arealization. This process is believed to be reliant on both activity-dependent and activity-independent mechanisms. Although both mechanisms are thought to function concurrently during arealization, the nature of their interaction is not understood. To examine the potential interplay of extrinsic activity-dependent mechanisms, such as sensory input, and intrinsic activity-independent mechanisms, including gene expression in mouse neocortical development, we performed bilateral enucleations in newborn mice and conducted anatomical and molecular analyses 10 days later. In this study, by surgically removing the eyes of the newborn mouse, we examined whether early enucleation would impact normal gene expression and the development of basic anatomical features such as intraneocortical connections and cortical area boundaries in the first 10 days of life, before natural eye opening. We examined the acute effects of bilateral enucleation on the lateral geniculate nucleus of the thalamus and the neocortical somatosensory-visual area boundary through detailed analyses of intraneocortical connections and gene expression of six developmentally regulated genes at postnatal day 10.</p> <p>Results</p> <p>Our results demonstrate short-term plasticity on postnatal day 10 resulting from the removal of the eyes at birth, with changes in nuclear size and gene expression within the lateral geniculate nucleus as well as a shift in intraneocortical connections and <it>ephrin A5 </it>expression at the somatosensory-visual boundary. In this report, we highlight the correlation between positional shifts in <it>ephrin A5 </it>expression and improper refinement of intraneocortical connections observed at the somatosensory-visual boundary in enucleates on postnatal day 10.</p> <p>Conclusions</p> <p>Bilateral enucleation induces a positional shift of both <it>ephrin A5 </it>expression and intraneocortical projections at the somatosensory-visual border in only 10 days. These changes occur prior to natural eye opening, suggesting a possible role of spontaneous retinal activity in area border formation within the neocortex. Through these analyses, we gain a deeper understanding of how extrinsic activity-dependent mechanisms, particularly input from sensory organs, are integrated with intrinsic activity-independent mechanisms to regulate neocortical arealization and plasticity.</p