Genotypic and phenotypic characteristics of vancomycin-resistant enterococcus isolated from clinical specimens of patients in shahrekord

Background and purpose: Enterococci are important agents of endocarditis and urinary tract infections. They acquire resistance against antimicrobial agents which causes difficulty in their treatment. This study aimed to determine the antimicrobial susceptibility of strains resistant to vancomycin and identification of resistance genes vanA and vanB Enterococcus strains isolated from teaching hospitals in 2014. Material and methods: Hundred and fifty isolates of Enterococcus were isolated from hospitals and the genus and species composition were identified using different biochemical methods. Antibiogram tests were also conducted. The minimum inhibitory concentration was conducted applying broth micro dilution. Finally the strains containing vanA and VanB genes were identified. Results: The antibiogram test showed 10 strains with VanA and VanB phenotype. All strains were resistant to ciprofloxacin. VanA and VanB genes were detected in all 10 isolates of vanA phenotype and two strains of vanB phenotype. VanA and VanB genes were proliferated by PCR in two strains of the10 strains that were VanA phenotype. Conclusion: Among Enterococcus species E. faecium and E. faecalis isolates were detected in teaching hospitals. The highest rate of resistance was to erythromycin and tetracycline and the lowest resistance rate was to vancomycin

The prevalence of methicillin-resistant Staph. Aureus strains producing enterotoxin A and B

Background: Staphylococcus aureus is a gram positive coccus which is able to cause different kinds of infection in certain condition. The function of this bacteria is to provide the conditions for the invasion of it to the host with the secretion of different sorts of toxins such as Staphylococcus aureus enterotoxin, including important virulence factors that super antigens are all factors digestive inconvenience. Staphylococcus aureus enterotoxin-secreting toxins such conditions provides invasion of host genes. There are different types of SE, but type A enterotoxin (SEA) and type B enterotoxin (SEB) are the most important types. Therefore, in this study, the prevalence of Staphylococcus aureus toxin-producing enterotoxin genes (SEB, SEA) in clinical strains isolated from patients in teaching hospitals of Shahrekord city, Iran, were studied. Methods: This cross-sectional and descriptive study, which was conducted from May 2014 to December 2014. A hundred and ten isolates of Staphylococcus aureus from patients collected over a period of 8 months and were first identified using standard biochemical methods and laboratory. Using standard methods and laboratory tests were identified and compared with the antibiotic oxacillin minimum inhibitory concentration were determined by broth micro dilution, and then they were assessed by polymerase chain reaction (PCR) technique. Results: The results indicated that, 110 samples of dairy products infected by Staphylococcus aureus were detected. Two cases (1.8%) of these infected samples were carrying both enterotoxin A and enterotoxin B genes. The frequencies of enterotoxin A genes were twenty-six cases (23/6%) and The frequencies of enterotoxin B genes were two cases (1/8%), respectively. Conclusion: The detection of enterotoxin A and enterotoxin B genes, shows the most important role they have in bringing about superinfection. The detection of enterotoxin A and B genes, shows the most important role they have in bringing about superinfection. Enterotoxins SEA and SEB are heat stable; therefore heating has no effect on dairy products contaminated by enterotoxins and gastritis may occur in a short period of time. As PCR is a rapid, sensitive, specific and inexpensive method, we suggest that it can be replaced to traditionally assays for detecting Staphylococcus aureus enterotoxin. © 2016, Tehran University of Medical Sciences. All rights reserved

Assessment of the Prevalence of Staphylococcus Areus in Nose of the Surgical Staff of Hajar and kashani's Hospital in 2015

زمینه و هدف: استافیلوکوک طلایی یکی از شایع ترین علل عفونت های بیمارستانی است. این مطالعه با هدف بررسی میزان فراوانی حاملین بینی استافیلوکوکوس اورئوس و ارتباط آن با آلودگی صورت گرفت. روش بررسی: در این مقاله تحقیقاتی کوتاه در مرحله ی اول، نمونه ها به صورت تصادفی از دست کارکنان بخش های مختلف بیمارستان کاشانی و هاجر شهرکرد اخذ شد و پس از کشت، نتایج توسط نرم افزار SPSS و آزمون های آماری کای اسکویر و دقیق فیشر مورد تجزیه و تحلیل قرار گرفت. یافته ها: از 90 نفری که در مرحله ی اول انتخاب شدند، 44 نفر (8/48) در هر دو نوبت، نمونه ی دست آلوده داشتند. ارتباط معنی داری از نظر آماری بین تعداد حاملین بینی و آلودگی دست ها مشاهده شد (0/05>P). نتیجه گیری: این مطالعه نشان داد که حاملین بینی استافیلوکوک طلایی می توانند باعث آلودگی دست ها به این باکتری شده و این امر به انتشار این باکتری به خصوص در محیط بیمارستانی کمک می نماید

Prevalence of constitutive and inducible resistance to clindamycin in staphylococci isolates from Hajar and Kashani hospitals in Shahrekord, 2014

چکیده مقدمه: مقاومت به کلیندامایسین در استافیلوکوکها به دو صورت بنیادی و القایی ایجاد می شود. درسویه هایی از این باکتریها که به اریترومایسین مقاوم هستند، ممکن است مقاومت القایی به کلیندامایسین نیز رخ دهد که با روش‌های معمول آنتی بیوگرام قابل تشخیص نیست. این مطالعه با هدف تعیین فنوتیپ های القایی مقاوم به کلیندامایسین در سویه‌های استافیلوکوک جدا شده ازبیماران بستری در بیمارستان‌های هاجر و کاشانی شهرکرد انجام شد. روش بررسی: این مطالعه توصیفی –تحلیلی برروی 200 ایزوله استافیلوکوکوس اورئوس و استافیلوکوک کواگولاز منفی مقاوم به متی سیلین که ازنمونه های بالینی بیماران جدا شده بودند با استفاده ازروش دیسک دیفیوژن انجام گرفت. مقاومت به کلیندامایسین درایزوله ها یی که به اریترومایسین مقاوم بودند، با ظهورهاله حساسیت به شکل D مشخص گردید. یافته‌ها: از بین 200 ایزوله استافیلوکوک مقاوم به متی سیلین، فنوتیپ D در ۶ ایزوله (3) (یک ایزوله استافیلوکوکوس اورئوس و ۵ ایزوله استافیلوکوک کواگولاز منفی) مشاهده شد. در چهار ایزوله فنوتیپ مثبت Dمشاهده شد. 13 ایزوله نیز فنوتیپ D منفی رانشان دادند. نتیجه گیری: تست تعیین مقاومتهای القایی روش مناسبی برای شناسایی الگوهای مقاومت در بین سویه های مختلف استافیلوکوک می باشد. به نظر می رسد انجام تست D درسویه های با فنوتیپ مقاوم به اریترومایسین ضروری بوده، وبا انجام این آزمایش می توان گزارش صحیح تری از حساسیت واقعی این سویه ها نسبت به کلیندامایسین ارائه داد. واژگان کلیدی: تست القا، کلیندامایسین، استافیلوکوکوس اورئوس، استافیلوکوکهای کوآگولازمنف

Antioxidant activity, total phenolic and flavonoid content, and antibacterial effects of Stachys lavandulifolia Vahl. Flowering shoots gathered from Isfahan

Plants are a rich source of phenolic compounds and one of the most important nature-based antioxidants. The compounds derived from plant-based extracts are an important pharmaceutical resource. This study was conducted to investigate the antioxidant activity and total phenolic and flavonoid content, and to investigate the antibacterial effects of Stachys lavandulifolia flowering shoots extract. In this study, S. lavandulifolia was gathered from Isfahan and extracted by maceration using ethanol 70%. Total phenol content was measured by Folin-Ciocalcteu reagent and gallic acid standard, and antioxidant activity was investigated by DPPH with reference to butylated hydroxytoluene (BHT). Antibacterial effects were investigated by Broth Microdilution and minimum inhibitory concentration and minimum bactericidal concentration were determined.The effects of different concentrations of S. lavandulifolia were investigated on Staphylococcus aureus and Enterococcus faecalis by disk diffusion with reference to vancomycin and nitrofurantoin. The findings demonstrated that the inhibition of DPPH free radicals was greater by hydroalcoholic S. aureus extract than BHT, and therefore the IC50 of this extract was lower than BHT. Total phenolic content was obtained 18.61 (mg gallic acid) and the flavonol and flavonoid content was obtained 2.42 and 8.93 mg/g, respectively. In this study, investigating the effects of different concentrations of hydroalcoholic S. lavandulifolia extract on pathogenic bacteria by disk diffusion and Broth Microdilution demonstrated that this extract exerted great inhibitory effects on both bacteria. S. aureus was more susceptible to S. lavandulifolia extract than E. faecalis

In Vitro Evaluation of Antioxidant Activity and Antibacterial Effects and Measurement of Total Phenolic and Flavonoid Contents of Quercus brantii L. Fruit Extract

Plant-based extracts, as alternatives to chemical compounds, are commonly use in pharmaceutical and food industries. Antibacterial properties of extracts are mainly considered in medicine. Because of the high incidence of infectious diseases, it is helpful to identify more agents that are able to treat diseases. Antioxidant effects have been reported for different plant extracts. We aimed to investigate antioxidant activity, total phenolic and flavonoid contents, and antibacterial effect of Quercus brantii L. fruit extract. In this study, Q. brantii L. fruit was extracted by maceration using ethanol 70%. Total phenolic content was determined by Folin-Ciocalceu reagent and gallic acid equivalence, and antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) compared to butylated hydroxytoluene (BHT). Antibacterial effects were investigated by broth microdilution and measurement of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Then the effects of different concentrations of extract on Staphylococcus aureus and Enterococcus faecalis growth were investigated in comparison with vancomycin and nitrofurantoin. Our findings demonstrate that antioxidant activity of hydroalcoholic extract of Q. brantii L. fruit was more than that of BHT. Total phenolic content was derived (3.010 mg GAE/g DW). Flavonol and flavonoid contents were 1.813 and 0.654 mg/g, respectively. The extract was found to exert significant inhibitory effects on both studied bacteria. S. aureus was more susceptible than E. faecalis to Q. brantii L. fruit extract. Q. brantii L. fruit extract can exert considerable antioxidant effects, contains high amounts of phenolic, flavonol, and flavonoid compounds, and inhibits bacterial growth. © 2018 Taylor & Francis Group, LL

In Vitro Evaluation of Antioxidant Activity and Antibacterial Effects and Measurement of Total Phenolic and Flavonoid Contents of Quercus brantii L. Fruit Extract

Plant-based extracts, as alternatives to chemical compounds, are commonly use in pharmaceutical and food industries. Antibacterial properties of extracts are mainly considered in medicine. Because of the high incidence of infectious diseases, it is helpful to identify more agents that are able to treat diseases. Antioxidant effects have been reported for different plant extracts. We aimed to investigate antioxidant activity, total phenolic and flavonoid contents, and antibacterial effect of Quercus brantii L. fruit extract. In this study, Q. brantii L. fruit was extracted by maceration using ethanol 70%. Total phenolic content was determined by Folin-Ciocalceu reagent and gallic acid equivalence, and antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) compared to butylated hydroxytoluene (BHT). Antibacterial effects were investigated by broth microdilution and measurement of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Then the effects of different concentrations of extract on Staphylococcus aureus and Enterococcus faecalis growth were investigated in comparison with vancomycin and nitrofurantoin. Our findings demonstrate that antioxidant activity of hydroalcoholic extract of Q. brantii L. fruit was more than that of BHT. Total phenolic content was derived (3.010 mg GAE/g DW). Flavonol and flavonoid contents were 1.813 and 0.654 mg/g, respectively. The extract was found to exert significant inhibitory effects on both studied bacteria. S. aureus was more susceptible than E. faecalis to Q. brantii L. fruit extract. Q. brantii L. fruit extract can exert considerable antioxidant effects, contains high amounts of phenolic, flavonol, and flavonoid compounds, and inhibits bacterial growth

Mutations in C2ORF71 Cause Autosomal-Recessive Retinitis Pigmentosa

With a worldwide prevalence of 1 in 4,000, retinitis pigmentosa (RP) is the most common form of hereditary retinal degeneration. More than 30 genes and loci have been implicated in nonsyndromic autosomal-recessive (ar) RP. Genome-wide homozygosity mapping was conducted in one Dutch and one Israeli family affected by arRP. The families were found to share a 5.9 Mb homozygous region on chromosome 2p23.1-p23.3. A missense variant in one of the genes residing in this interval, C2ORF71, has recently been reported to be associated with RP. C2ORF71, encoding a putative protein of 1,288 amino acids, was found to be specifically expressed in human retina. Furthermore, RT-PCR analysis revealed that in the mouse eye, C2orf71 is expressed as early as embryonic day 14. Mutation analysis detected a 1 bp deletion (c.946 del; p.Asn237MetfsX5) segregating with RP in the Dutch family, whereas a nonsense mutation (c.556C > T; p.Gln186X) was identified in the Israeli family. Microsatellite-marker analysis in additional Israeli families revealed cosegregation of a C2ORF71-linked haplotype in one other family, in which a 13 bp deletion (c.2756_2768 del; p.Lys919ThrfsX) was identified. Clinically, patients with mutations in C2ORF71 show signs of typical RP; these signs include poor night vision and peripheral field loss, typical retinal bone-spicule-type pigment deposits, pale appearance of the optic disk, and markedly reduced or completely extinguished electroretinograms. In conclusion, truncating mutations in C2ORF71 were identified in three unrelated families, thereby confirming the involvement of this gene in the etiology of arRP