Qualitative analysis of meat and meat products by multiplex polymerase chain reaction (PCR) technique

Multiplex polymerase chain reaction (M-PCR) assay was applied to processed and raw meats for the identification of the most used species in foodstuffs such as, ruminant, poultry, fish and pork materials. Specific-species primers, designed according to the conservative regions of 16S rRNA, were used after alignment of the available sequences in the GenBank database. The primers generated specific DNA fragments of 183, 224, 290 and 374 bp length for poultry, fish, pork and ruminant, respectively. The optimized M-PCR assay was applied to 93 commercial meat products and it showed the presence of poultry meat in red meat analyzed, although, it was not indicated on the label.Key words: Multiplex polymerase chain reaction (M-PCR), meat products, food, salami, sausage

Immobilization of urease on copper chelated EC-Tri beads and reversible adsorption

In the present study, Eupergit C® macroporous beads were functionalized with amino triazole and characterized by FTIR-ATR and SEM. Cu2+ ions were chelated on the triazole modified Eupergit C® (EC®), and then the metal chelated beads were used in the adsorption of urease. Maximum reaction rate (Vmax) and Michaelis-Menten constant (km) were determined for the free and immobilized enzymes. Various characteristics of immobilized urease such as the temperature activity curve, thermal stability, operational stability and storage stability were evaluated. The results demonstrated that triazole functionalized Eupergit C® beads can be applied to metal sorption and enzyme immobilization.Key words: Urease, immobilization, Eupergit C®, triazole, chelating beads

Acetone soluble mutagenicity assessment tested by a salmonella/microsome system (AMES) in the strait of marmara (Black Sea and the Sea of Marmara)

Many researches have been conducted on various parameters related to the pollution in the Black Sea, the Sea of Marmara and the Strait of Istanbul. However, there is no report on the mutagenicity tested by evaluating the mutagenic effects of the total pollution, dissolving water samples in acetone, and using a salmonella/microsome test system

Re-exploring Planaria as a model organism for genotoxicity monitoring by an "improved random amplified polymorphic DNA (RAPD)*" approach

In monitoring genotoxicity, it is important to have sensitive, but non-specific assays to indicate a wide range of DNA damage mechanisms. In this paper, a new and flexible approach for the qualitative detection of induced DNA effects, DNA damages and mutations, accelerated by exploiting planaria regeneration and using an improved RAPD assay, is presented

A preliminary report on target organ genotoxicity biomonitoring by an "improved random amplified polymorphic DNA" assay

Genetic changes, genotoxicity and even target organ genotoxicity can be monitored by using RAPD assays that have a great potential in detecting DNA effects as well as pathologic and/or physiologic ones, when as little as 2-5% of the cells have the same affected genotype