36 research outputs found
G-CSF receptor truncations found in SCN/AML relieve SOCS3-controlled inhibition of STAT5 but leave suppression of STAT3 intact
Truncated granulocyte colony-stimulating factor receptors (G-CSF-Rs) are
implicated in severe congenital neutropenia (SCN) and the consecutive
development of acute myeloid leukemia (AML). Mice expressing G-CSF-R
truncation mutants (gcsfr-d715) show defective receptor internalization,
an increased signal transducer and activator of transcription 5
(STAT5)/STAT3 activation ratio, and hyperproliferative responses to G-CSF
treatment. We determined whether a lack of negative feedback by suppressor
of cytokine signaling (SOCS) proteins contributes to the signaling
abnormalities of G-CSF-R-d715. Expression of SOCS3 transcripts in bone
marrow cells from G-CSF-treated gcsfr-d715 mice was approximately 60%
lower than in wild-type (WT) littermates. SOCS3 efficiently suppressed
STAT3 and STAT5 activation by WT G-CSF-R in luciferase reporter assays. In
contrast, while SOCS3 still inhibited STAT3 activation by G-CSF-R-d715,
STAT5 activation was no longer affected. This was due mainly to loss of
the SOCS3 recruitment site Tyr729, with an additional contribution of the
internalization defects of G-CSF-R-d715. Because Tyr729 is also a docking
site for the Src homology 2-containing protein tyrosine phosphatase-2
(SHP-2), which binds to and inactivates STAT5, we suggest a model in which
reduced SOCS3 expression, combined with the loss of recruitment of both
SOCS3 and SHP-2 to the activated receptor complex, determine the increased
STAT5/STAT3 activation ratio and the resulting signaling abnormalities
projected by truncated G-CSF-R mutants
Plaats delict-onderzoek met vooruitziende blik: Anticiperen op alternatieve scenario’s tijdens het sporenonderzoek op de plaats delict
Criminal Justice: Legitimacy, accountability, and effectivit
Inhibition of intercellular communication by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): possible role of the Ah-receptor.
Targeting relevant sampling areas for human biological traces:Where to sample displaced bodies for offender DNA?
Targeting relevant sampling areas for human biological traces:Where to sample displaced bodies for offender DNA?
Inhibition of intercellular communication by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): possible role of the Ah-receptor.
Human NAD(P)H:Quinone oxidoreductase inhibition by flavonoids in living cells
Procedures for assessing enzyme inhibition in living cells are an important tool in the study of the relevance of enzyme-catalyzed reactions and interactions in the human body. This paper presents the effects of flavonoids on NAD(P)H:quinone oxidoreductase 1 (NQO1) activity, by a newly developed method to measure NQO1 inhibition in intact cells. The principle of this method is based on the resorufin reductase activity of NQO1. The change in fluorescence in time was used to determine NQO1 activity in intact Chinese hamster ovary (CHO) cells genetically engineered to overexpress human NQO1. Applying this method to determine the inhibitory effects of reported in vitro NQO1 inhibitors (dicoumarol, 7,8-dihydroxyflavone, chrysin) showed that for all inhibitors tested, the IC50 in intact cells was at least 3 orders of magnitude higher than the IC50 in cell lysates. This result demonstrates that in vitro studies with purified NQO1 or with extracts from disrupted tissues are of limited value for obtaining insight into the situation in living cells. Possible factors underlying this discrepancy are being discussed. For the first time, we determined NQO1 inhibition by flavonoids in cells without disruption of the cells or addition of cofactors, enabling the assessment of enzymatic activity and the interaction of modulators of enzymatic activity in an intracellular situation
B 50/GAP 43 induced formation of filopodia depends on rho GTPase
In the present study we show that expression of the neural PKC-substrate B-50 (growth-associated protein [GAP-43]) in Rat-1 fibroblasts induced the formation of filopodial extensions during spreading. This morphological change was accompanied by an enhanced formation of peripheral actin filaments and by accumulation of vinculin immunoreactivity in filopodial focal adhesions, colocalizing with B-50. In time lapse experiments, the B-50induced filopodial extensions were shown to stay in close contact with the substratum and appeared remarkably stable, resulting in a delayed lamellar spreading of the fibroblasts. The morphogenetic effects of the B-50 protein were entirely dependent on the integrity of the two N-terminal cysteines involved in membrane association (C3C4), but were not significantly affected by mutations of the PKC-phosphorylation site (S41) or deletion of the C terminus (177226). Cotransfection of B-50 with dominant negative Cdc42 or Rac did not prevent B-50induced formation of filopodial cells, whereas this process could be completely blocked by cotransfection with dominant negative Rho or Clostridium botulinum C3-transferase. Conversely, constitutively active Rho induced a similar filopodial phenotype as B-50. We therefore propose that the induction of surface extensions by B-50 in spreading Rat-1 fibroblasts depends on Rho-guanosine triphosphatase function