11 research outputs found
Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in interior Alaska
To improve knowledge of ground beetle communities and the influence of habitat succession on these communities in Alaska, adult ground beetle (Coleoptera: Carabidae) activity and diversity was documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145ºW). Twenty species, based on a total sample of 6,116 specimens, were collected during 2006 and 2007 from plots that were in the CRP for 9 years (young-field plots) and 19 years (old-field plots). Two species, Cymindis cribricollis Dejean and Amara obesa Say, are reported for the first time for Alaska. Species richness of carabids for our study plots was estimated using the Chao 1 and Chao 2 estimators to be 22 and 28 species, respectively. Ninety-four percent of the specimens belonged to five species Pterostichus adstrictus Eschscholtz (42.9%), Agonum cupreum Dejean (17.9%), Calathus ingratus Dejean (15%), Amara obesa (11.1%), and Dicheirotrichus cognatus (Gyllenhal) (7.1%). Only Ag. cupreum showed significant effects based on plot age. The majority of carabid activity occurred late in the season, from mid September to early October. A comparison of our findings is made with historical data (1943-1956) from the collection of the Matanuska Experiment Station now incorporated into the University of Alaska Museum Insect Collection
Lady beetles (Coleoptera: Coccinellidae: Coccinellini) associated with Alaskan agricultural crops
Adult coccinellid abundance was monitored in agricultural areas of the Tanana and Matanuska—Susitna River Valleys of Alaska during 2004 and 2005. Thirteen species were collected in association with Alaskan agricultural crops. Of the species collected, Hippodamia quinquesignata quinquesignata (Kirby), Coccinella transversoguttata richardsoni Brown, and Hippodamia tredecimpunctata tibialis (Say) were by far the most abundant species, making up 51, 18, and 12%, respectively, of the individuals collected. Two new species, Coccinella septempunctata L. and Hippodamia convergens Guerin, were recorded for the first time in Alaska
Ground beetle (Coleoptera: Carabidae) assemblages in the Conservation Reserve Program crop rotation systems in interior Alaska
To improve knowledge of ground beetle communities and the influence of habitat succession on these communities in Alaska, adult ground beetle (Coleoptera: Carabidae) activity and diversity was documented on Conservation Research Program (CRP) agricultural lands in Delta Junction, Alaska (64ºN, 145ºW). Twenty species, based on a total sample of 6,116 specimens, were collected during 2006 and 2007 from plots that were in the CRP for 9 years (young-field plots) and 19 years (old-field plots). Two species, Cymindis cribricollis Dejean and Amara obesa Say, are reported for the first time for Alaska. Species richness of carabids for our study plots was estimated using the Chao 1 and Chao 2 estimators to be 22 and 28 species, respectively. Ninety-four percent of the specimens belonged to five species Pterostichus adstrictus Eschscholtz (42.9%), Agonum cupreum Dejean (17.9%), Calathus ingratus Dejean (15%), Amara obesa (11.1%), and Dicheirotrichus cognatus (Gyllenhal) (7.1%). Only Ag. cupreum showed significant effects based on plot age. The majority of carabid activity occurred late in the season, from mid September to early October. A comparison of our findings is made with historical data (1943-1956) from the collection of the Matanuska Experiment Station now incorporated into the University of Alaska Museum Insect Collection
Aphids (Hemiptera: Aphididae) associated with rhubarb (Rheum spp.) in the Matanuska Valley, Alaska: species composition, seasonal abundance, and potential virus vectors
Culinary rhubarb, Rheum spp., is one of the priority crop species curated by the United States Department of Agriculture (USDA) Agricultural Research Service (ARS) in Palmer, Alaska. Water—pan traps in commercial rhubarb in the Matanuska—Susitna River Valley near Palmer and in the USDA ARS Rheum gerrnplasm collection caught aphids belonging to eight species: Aphis helianthi Monell; Chaitophorus neglectus Hottes and Friso, Euceraphis betulae (Koch); Hayhurstia atriplicis (L.); Macrosiphum euphorbiae (Thomas); Myzus persicae (Sulzer); Pemphigus spp.; and Rhopalosiphum padi (L.). Only three of the species (M. euphorbiae, M. persicae, and R. padz) collected in water— pan traps were also handpicked from rhubarb plants. The bird cherry—oat aphid, R. padi, was the most abundant species collected in water-pan traps and from rhubarb plants. Based on their disease transmission capability, A. helianthi, M. euphorbiae, M. persicae, and R. padi, can be considered to be of potential economic importance to rhubarb production in Alaska
Transformations to granular zircon revealed: Twinning, reidite, and ZrO2 in shocked zircon from Meteor Crater (Arizona, USA)
Granular zircon in impact environments has long been recognized but remains poorly understood due to lack of experimental data to identify mechanisms involved in its genesis. Meteor Crater in Arizona (USA) contains abundant evidence of shock metamorphism, including shocked quartz, the high-pressure polymorphs coesite and stishovite, diaplectic SiO2 glass, and lechatelierite (fused SiO2). Here we report the presence of granular zircon, a new shocked-mineral discovery at Meteor Crater, that preserve critical orientation evidence of specific transformations that occurred during formation at extreme impact conditions. The zircon grains occur as aggregates of sub-micrometer neoblasts in highly shocked Coconino Sandstone (CS) comprised of lechatelierite. Electron backscatter diffraction shows that each grain consists of multiple domains, some with boundaries disoriented by 65° around <110>, a known {112} shock-twin orientation. Other domains have {001} in alignment with {110} of neighboring domains, consistent with the former presence of the high-pressure ZrSiO4 polymorph reidite. Additionally, nearly all zircon preserve ZrO2 + SiO2, providing evidence of partial dissociation. The genesis of CS granular zircon started with detrital zircon that experienced shock twinning and reidite formation at pressures from 20 to 30 GPa, ultimately yielding a phase that retained crystallographic memory; this phase subsequently recrystallized to systematically oriented zircon neoblasts, and in some areas partially dissociated to ZrO2. The lechatelierite matrix, experimentally constrained to form at >2000 °C, provided the ultrahigh-temperature environment for zircon dissociation (~1670 °C) and neoblast formation. The capacity of granular zircon to preserve a cumulative pressure-temperature record has not been recognized previously, and provides a new method for investigating histories of impact-related mineral transformations in the crust at conditions far beyond those at which most rocks melt
Fluorescence Linked Enzyme Chemoproteomic Strategy for Discovery of a Potent and Selective DAPK1 and ZIPK Inhibitor
DAPK1 and ZIPK (also called DAPK3)
are closely related serine/threonine
protein kinases that regulate programmed cell death and phosphorylation
of non-muscle and smooth muscle myosin. We have developed a fluorescence
linked enzyme chemoproteomic strategy (FLECS) for the rapid identification
of inhibitors for any element of the purinome and identified a selective
pyrazolo[3,4-<i>d</i>]pyrimidinone (HS38) that inhibits
DAPK1 and ZIPK in an ATP-competitive manner at nanomolar concentrations.
In cellular studies, HS38 decreased RLC20 phosphorylation. In <i>ex vivo</i> studies, HS38 decreased contractile force generated
in mouse aorta, rabbit ileum, and calyculin A stimulated arterial
muscle by decreasing RLC20 and MYPT1 phosphorylation. The inhibitor
also promoted relaxation in Ca<sup>2+</sup>-sensitized vessels. A
close structural analogue (HS43) with 5-fold lower affinity for ZIPK
produced no effect on cells or tissues. These findings are consistent
with a mechanism of action wherein HS38 specifically targets ZIPK
in smooth muscle. The discovery of HS38 provides a lead scaffold for
the development of therapeutic agents for smooth muscle related disorders
and a chemical means to probe the function of DAPK1 and ZIPK across
species
Fluorescence Linked Enzyme Chemoproteomic Strategy for Discovery of a Potent and Selective DAPK1 and ZIPK Inhibitor
DAPK1 and ZIPK (also called DAPK3)
are closely related serine/threonine
protein kinases that regulate programmed cell death and phosphorylation
of non-muscle and smooth muscle myosin. We have developed a fluorescence
linked enzyme chemoproteomic strategy (FLECS) for the rapid identification
of inhibitors for any element of the purinome and identified a selective
pyrazolo[3,4-<i>d</i>]pyrimidinone (HS38) that inhibits
DAPK1 and ZIPK in an ATP-competitive manner at nanomolar concentrations.
In cellular studies, HS38 decreased RLC20 phosphorylation. In <i>ex vivo</i> studies, HS38 decreased contractile force generated
in mouse aorta, rabbit ileum, and calyculin A stimulated arterial
muscle by decreasing RLC20 and MYPT1 phosphorylation. The inhibitor
also promoted relaxation in Ca<sup>2+</sup>-sensitized vessels. A
close structural analogue (HS43) with 5-fold lower affinity for ZIPK
produced no effect on cells or tissues. These findings are consistent
with a mechanism of action wherein HS38 specifically targets ZIPK
in smooth muscle. The discovery of HS38 provides a lead scaffold for
the development of therapeutic agents for smooth muscle related disorders
and a chemical means to probe the function of DAPK1 and ZIPK across
species