Preliminary Studies on Piliostigma thonningii seeds: Proximate analysis, mineral composition and phytochemical screening

Qualitative determination of chemical and nutritional composition of Piliostigma thonningii, an underexploited crop seed in Nigeria, was carried out. Seeds of P. thonningii were found to be rich in crude protein, carbohydrate, and mineral elements. Mineral analysis of P. thonningii showed the seed as good source of antioxidant micronutrients such as iron, calcium, selenium, zinc and manganese. The phytochemical screening of the seed showed the presence of saponins, flavonoids, phenolics, glycosides, anthraquinones as well as cardiac glycosides while tannins, steroids, phylobatannins and triterpenes were absent. Although the oil has a very low peroxide value and high iodine value, it cannotbe recommended for consumption because of the low yield as well as its repulsive odour. The seed, however, can serve as a cheap source of protein, energy, as well as antioxidant micronutrients supplements in both man and animal

Effect of delayed sterilization on the production of intravenous fluids (parenterals)

The effect of delayed sterilization on the production of intravenous fluids using 5% (w/v) dextrose solution was studied. The 5% (w/v) dextrose was prepared with distilled water. The solution was inoculated with a  laboratory isolate of Escherichia coli and thereafter divided into two: Solutions A and B. Sample A was  sterilized within 1 h of innoculation, while B was incubated at 37‹C for 48 h and sterilized after. The microbial  load was determined before sterilization. Both samples were then tested for pyrogenicity using rabbit test of  pyrogen. The results revealed a significant (P < 0.05) increase in E. coli count in solution B from 8 } 2 to 99 } 5 cfu/ml after 48 h. Sample A became pyrogen-free after sterilization (sum of response (SR) of 3 rabbits,  0.75‹C), while sample B contained 40 EU/ml (limit: . 0.5 EU/ml for large volume parenteral). A 10 times dilution of sample B with pyrogen-free 0.9% w/v sodium chloride produced a response of 2.80‹C and SR of 3 rabbits (failed limit . 2.65‹C). From this study, a delay of up to 48 h before sterilizing solutions intended for parenteral use could produce high pyrogenicity.Key words: Parenterals, pyrogen, Escherichia coli, sterilization, microbial load

Compositional changes in banana (Musa ssp.) fruits during ripening

The compositional changes in banana (musa spp) fruits were investigated. Banana fruits were collected, dried, ground and ashed. The moisture content and mineral elements composition wasdetermined as ripening proceeds. The mineral elements analyzed included magnesium (Mg), manganese (Mn), zinc (Zn) and cobalt (Co). Their composition was found to be 0.68, 0.66 and 0.60% inunripe, ripe and overripe banana fruits, respectively. The moisture content and ash values for the selected mineral elements were 73.47 and 0.68%; 77.19 and 0.80%; 79.22 and 0.78% in unripe, ripe andoverripe banana fruits, respectively. The results showed that the nutritional composition of banana pulp was diversely affected by ripening. Changes in mineral composition varied and were not consistent with the stages of ripeness. Bananas are considered a good source of Mg in the diet, and the data obtained herein support these assertions. Zn and Mn are other minerals of nutritional importance inbananas and this study has shown that their average values are adequate to support its nutritive value at the various ripening stages. The result obtained in this study showed that banana fruits at anyripening stage (unripe, ripe or overripe) can be a potential source of mineral elements supplement in the diet especially for Mg

Frequency of anxiety after stroke: a systematic review and meta-analysis of observational studies.

BACKGROUND AND PURPOSE: Negative psychological outcomes occur frequently after stroke; however, there is uncertainty regarding the occurrence of anxiety disorders and anxiety symptoms after stroke. A systematic review of observational studies was conducted that assessed the frequency of anxiety in stroke patients using a diagnostic or screening tool. SUMMARY OF REVIEW: Databases were searched up to March 2011. A random effects model was used to summarize the pooled estimate. Statistical heterogeneity was assessed using the I(2) statistic. Forty-four published studies comprising 5760 stroke patients were included. The overall pooled estimate of anxiety disorders assessed by clinical interview was 18% (95%confidence interval 8-29%, I(2)  = 97%) and was 25% (95% confidence interval 21-28%, I(2)  = 90%) for anxiety assessed by rating scale. The Hospital Anxiety and Depression Scale-Anxiety subscale 'probable' and 'possible' cutoff scores were the most widely used assessment criteria. The combined rate of anxiety by time after stroke was: 20% (95% confidence interval 13-27%, I(2)  = 96%) within one-month of stroke; 23% (95% confidence interval 19-27%, I(2)  = 84%) one to five-months after stroke; and 24% (95% confidence interval 19-29%, I(2)  = 89%) six-months or more after stroke. CONCLUSION: Anxiety after stroke occurs frequently although methodological limitations in the primary studies may limit generalizability. Given the association between prevalence rates and the Hospital Anxiety and Depression Scale-Anxiety cutoff used in studies, reported rates could in fact underrepresent the extent of the problem. Additionally, risk factors for anxiety, its impact on patient outcomes, and effects in tangent with depression remain unclear