537 research outputs found

    Methanogens and methane oxidizing bacteria in forested, urban unrestored, and urban restored streams

    Get PDF
    Microbial methanogenesis, carried out by methanogens, and methane oxidation, carried out by methanotrophs, are integral parts of the carbon cycle and global climate change. These two processes have received attention in recent years in aquatic systems including major rivers, lakes, wetlands, and oceans. However, far less attention has been paid to flowing waters, especially low order streams, and to the potential impact of methane cycle processes in these environments. Streams are important to carbon cycling because as connectors to terrestrial ecosystems they are sites of organic matter processing, and important to biogeochemical cycling across the landscape. Twelve streams in the piedmont of North Carolina, consisting of three stream types: forested, urban unrestored, and urban restored, were sampled seasonally. Genomic DNA from seston, sediment, and epilithon samples was extracted and real-time PCR performed to analyze the abundance of methanogens and methanotrophs using group specific primers. Water samples were taken to measure methane concentration. There were significant differences in methanogen DNA abundance in late fall and methanotroph DNA abundance in late summer between stream types superimposed on a background of seasonal differences. The differences may be explained by the timing and magnitude of organic matter and inorganic matter inputs in forested versus urban streams. Urban unrestored streams receive a large pulse of input in late fall and little through the winter because stormwater is piped in directly to the streams from impervious surfaces; in contrast forested streams receive organic input over time because it enters the stream through subsurface flow

    Effects of a nutrition-based health promotion program on nutritional adequacy, planned physical activity, body composition, job performance, and absenteeism among female airline reservationists

    Get PDF
    A quasi-experimental, nonequivalent control group, pretest-posttest study was conducted with 84 female reservation employees of Piedmont Airlines in Winston-Salem, North Carolina, and Nashville, Tennessee. The purposes were to assess: the dietary levels of kilocalories and certain nutrients including protein, vitamin A, vitamin C, iron, calcium, thiamin, riboflavin and niacin; hemoglobin; time spent in planned physical activity; cardiovascular fitness measured by heart rate after exercise; body composition by percentage of body fat, Body Mass Index, and percentage of desirable body weight; job performance and absenteeism; and to evaluate the effectiveness of a nutrition-based health promotion program in achieving improvements in nutritional adequacy, hemoglobin, time spent in planned physical activity, body composition, job performance and absenteeism. The subjects included only full-time employees, ranging in age from 19 to 60 years. The Winston-Salem group served as the experimental group and were involved in a seven-month worksite nutrition-based health promotion program which emphasized basic nutrition, the dietary guidelines, stress control, and exercise. The Nashville group served as the control group and they were not exposed to the health promotion program

    Effects of a single aerobic exercise bout on gene regulation in the soleus muscle of Sprague Dawley rats

    Get PDF
    "The purpose of this study was to identify changes in gene transcription that occur in the soleus muscle of untrained, 10 week old rats following a single aerobic exercise bout of 2 hours. Of particular interest were genes involved in the production or protection from RONS. Rats were either run (experimental) for 2 hours or were rested (controls) and were killed 1 hour post-exercise with controls killed at a matched time. The soleus muscles from each animal were pooled and examined using DNA microarray technology. The microarray identified 52 genes significantly different between the conditions. The major gene families altered were metabolism (~10% of all genes altered), apoptosis (~8% of all genes altered), muscle contraction (~10% of all genes altered), transcription/cell signaling (~17%of all genes altered), tissue generation (~15.5% of all genes altered), and inflammation (~10% of all genes altered. To confirm the findings of specific genes from the microarray, real time PCR was performed on 4 genes of interest (NFκB, TNFα, Atf3, and Mgst1). The results from the PCR analysis on these 4 genes were consistent with the microarray results. NFκB and TNFα were not significantly altered on the microarray or PCR analysis, whereas Atf3 (up-regulated) and Mgst1 (down-regulated) were found to be significantly altered in exercised animals by both methods. Genes related to RONS protection were down-regulated. Western Blot analysis was used to check if protein level was correspondingly altered. The process from gene transcription to protein translational is a complex process that may not coincide. NFκB protein level was not different between the 2 groups, matching the results obtained with NFκB gene level. Atf-3 protein level was elevated in the exercise group, matching the results obtained with Atf-3 gene level. These data suggest that one hour after a 2 hour run at ~65% of VO2 max, the soleus muscle undergoes changes in gene expression. These significant changes occurred in the areas of metabolism, apoptosis, muscle contraction, transcription/cell signaling, tissue generation, and inflammatory genes. Transcription of many genes involved in production of RONS was elevated, while that of genes involved in RONS protection were either depressed or unaltered."--Abstract from author supplied metadata

    Perchloric acid catalyzed acylations

    Get PDF
    The object of this work was to explore the generality of acetic anhydride-perchloric acid catalyzed C-acylations, to determine experimental conditions which maximize C-acylation, and to further elucidate the mechanism by which the C-acylation occurs. The ability of acetic anhydride-perchloric acid systems to effect C-acylation of cyclohexanone, 3-methylcyclohexanone, and 4-methylcyclohexanone was demonstrated; the C-acylation yields are 36%, 40%, and 36% respectively. A reaction time of three hours with 1M acetic anhydride and 10-1M perchloric acid in a solvent of low dielectric constant and weak electron donating power such as chloroform gives maximum C-acylation. The molar ratio of 1:10:1 for ketone : acetic anhydride : perchloric acid was found best for C-acylation. Tar formation is a considerable problem and material balance studies indicate from 65% - 82% recovery of material. Several mechanistic possibilities are examined including: direct C-acylation of the enol form of the ketone, C-acylation of the enol acetate, and rearrangement of the enol acetate as possible mechanisms for the C-acylation of ketones in acetic anhydrideperchloric acid catalyzed systems. The direct C-acylation of the enol form of the ketone is found to be the mechanism most consistent with all the experimental data

    “Robin Hood of the Blue Ridge”: The Life, Legend, and Songs of Otto Wood, the Bandit

    Get PDF
    Otto Wood, a native of Wilkes County, North Carolina, became nationally known during the 1920s for his repetitive flights from the North Carolina State Prison. Wood began his rambling at an early age and spent his childhood years in the coalfields of southern West Virginia. After killing a Greensboro pawnbroker in the fall of 1923, he was sent to the North Carolina State Prison. Between 1924 and 1930, Wood made four escapes from the penitentiary and rose to the status of a criminal celebrity. He wrote his autobiography, Life History of Otto Wood, while incarcerated in 1926. In his Life History, Wood claimed that the poverty and neglect he experienced in childhood formed the roots of his criminal lifestyle. Governor O. Max Gardner attempted to use Wood as an “experiment in humanity,” but failed after Wood made his fourth escape in 1930. He died on December 31, 1930, following a gunfight with police on the streets of Salisbury, North Carolina. Wood’s legend was later spread in song by early country music artists. Drawing on primary sources, this thesis provides a biography of Otto Wood and places him within the economic and social context of the period in which he lived

    On a mission : Grace Ellison's An Englishwoman in a Turkish harem

    Get PDF
    This thesis, entitled “On A Mission: Grace Ellison’s An Englishwoman in a Turkish Harem,” closely analyzes the rhetoric that is used within Ellison’s text. Ellison writes about her adventures in Turkey from a uniquely British perspective. Her discourse addresses such issues as the veil, the harem and women’s rights. Ellison’s socio-political concerns, she claims, are centered on the Turkish women. Often times though, her discourse shifts to address the progress and needs of Englishwomen of the early twentieth century. This intimate study of An Englishwoman in a Turkish Harem reveals Ellison’s progressive feminist ideas as well as the fervent nationalistic propaganda that she espouses in order to champion her cause

    Amplifying teacher voice in education policy discussions: The case of North Carolina

    Get PDF
    The researcher of this disquisition will examine teachers’ beliefs in their ability to advocate for the profession as well as, can be extended to serve as a vehicle for teachers to dialogue with state policymakers. , exclusively using technology. Tar Heel Teachers at Home is a North Carolina interview series that is hosted by a panel of educators. The series is focused on engaging North Carolinians in conversations around education. Most of the decision makers who have appeared on the show have been individuals with local jurisdiction such as system superintendents and school board members. Members of the General Assembly represent less than 7% of featured guests on the program since it was launched in 2019. Since 2010, North Carolina’s educational landscape has been most impacted by laws that were initiated by the General Assembly. Career status protections have been abolished, compensation for advanced degrees have been terminated, the cap on the number of charter schools has been lifted, and the private school voucher program has been expanded (Parmenter, 2019). These wholesale changes to public education affect collective teacher perception of the profession as well as their ability to have a positive impact. The General Assembly should be a central component because of its influence in the state. Throughout the close examination of this problem, all featured guests on Tar Heel Teachers at Home will be members of the General Assembly (NC House of Representatives & NC Senators). Participants will be invited to interact with the General Assembly across multiple technology avenues. Teacher voice is not totally absence from educational policy discussions in North Carolina. However, there is a need to amplify teacher voice because more and more decisions are being made at the local, district, and state levels that could impact students in a more positive way if teacher voice was included and taken into consideration. The purpose of this improvement initiative was to improve school climate, positively impact student achievement, and reduce teacher turnover. Research shows that there are major benefits for schools in these three areas when teachers are included in the decision-making process (Kahlenburg and Potter, 2018)
    • …
    corecore