278 research outputs found
Theoretical limits on magnetic field strengths in low-mass stars
19 pages, 10 figures, accepted to ApJObservations have suggested that some low-mass stars have larger radii than predicted by 1-D structure models. Some theoretical models have invoked very strong interior magnetic fields (of order 1 MG or more) as a possible cause of such large radii. Whether fields of that strength could in principle by generated by dynamo action in these objects is unclear, and we do not address the matter directly. Instead, we examine whether such fields could remain in the interior of a low mass object for a significant time, and whether they would have any other obvious signatures. First, we estimate timescales for the loss of strong fields by magnetic buoyancy instabilities. We consider a range of field strengths and simple morphologies, including both idealized flux tubes and smooth layers of field. We confirm some of our analytical estimates using thin flux tube magnetohydrodynamic (MHD) simulations of the rise of buoyant fields in a fully-convective M-dwarf. Separately, we consider the Ohmic dissipation of such fields. We find that dissipation provides a complementary constraint to buoyancy: while small-scale, fibril fields might be regenerated faster than they rise, the dissipative heating associated with such fields would in some cases greatly exceed the luminosity of the star. We show how these constraints combine to yield limits on the internal field strength and morphology in low-mass stars. In particular, we find that for stars of 0.3 solar masses, no fields in flux tubes stronger than about 800 kG are simultaneously consistent with both constraints
Envolvimento de "stakeholders" em direcção à construção sustentável
A mudança de paradigma requerida para evoluir na direcção da construção sustentável exige a conjugação efectiva de diferentes condições determinantes, que inclui a percepção, comportamento e envolvimento de partes em todo o processo, em particular os actores que efectam, bem como aqueles que são afectados pela tomada de decisão. Para além das formas convencionais de envolvimento dos stokholders, novas formas de cooperação precisam de ser exploradas. O presente artigo tem por obectivo reflectir sobre a melhor estratégia de gestão a aplicar nos actores-chave num contexto de networking sobre construção sustentável em Portugal. A análise efectuada permitiu verificar que o sucesso de uma plataforma de cooperação está condicionada pela participação dos actores-chave, pelo que há necessidade de fazer o mapeamento, a hierarquização e a definição de uma estratégia de gestão que maximize asua cooperação, isto é, que vá ao encontro das necessidades de cada um deles, para que a plataforma de cooperação se torne um efectivo espaço de partilha, diálogo, aprendizagem e inovação
Morris dancers, matriarchs and paperbacks:Doing the village in contemporary Britain
To call a place rural is to categorize it as a particular kind of place and, often, to presume that particular kinds of being innately occur there. Over the past 20 years, however, trends in British rural studies have problematized easy ascription; this article is an ethnographic contribution within those trends. If it is no longer adequate to read the rural as a container for being, then, as I contend here, rurality can be explored anew through doing. I draw upon David Matless’s (1994) frame of ‘doing the village’ representationally, and amplify it to include concepts of place as representational and relational. I thus use ‘doing’ to read the multiple ways in which diverse residents in a Northern England village engage with both their real locality and with nationally shared rural imaginings
Morphogenesis of Strongyloides stercoralis Infective Larvae Requires the DAF-16 Ortholog FKTF-1
Based on metabolic and morphological similarities between infective third-stage larvae of parasitic nematodes and dauer larvae of Caenorhabditis elegans, it is hypothesized that similar genetic mechanisms control the development of these forms. In the parasite Strongyloides stercoralis, FKTF-1 is an ortholog of DAF-16, a forkhead transcription factor that regulates dauer larval development in C. elegans. Using transgenesis, we investigated the role of FKTF-1 in S. stercoralis' infective larval development. In first-stage larvae, GFP-tagged recombinant FKTF-1b localizes to the pharynx and hypodermis, tissues remodeled in infective larvae. Activating and inactivating mutations at predicted AKT phosphorylation sites on FKTF-1b give constitutive cytoplasmic and nuclear localization of the protein, respectively, indicating that its post-translational regulation is similar to other FOXO-class transcription factors. Mutant constructs designed to interfere with endogenous FKTF-1b function altered the intestinal and pharyngeal development of the larvae and resulted in some transgenic larvae failing to arrest in the infective stage. Our findings indicate that FKTF-1b is required for proper morphogenesis of S. stercoralis infective larvae and support the overall hypothesis of similar regulation of dauer development in C. elegans and the formation of infective larvae in parasitic nematodes
Using functional genomics to decipher the complexity of microbial pathogenicity
From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of infection. Despite these limitations such work has facilitated the development of successful therapeutic strategies, most notably vaccines that target specific virulence-related features. There are however many antimicrobial resistant pathogens for which vaccination strategies have not worked, as we simply do not know enough about how they cause disease. We are however at the dawn of a new era in the study of microbial pathogenicity, where large collections of bacteria isolated directly from human infections can be sequenced and assayed to identify the bacterial features that affect disease severity in humans. Here, we describe our attempt to perform such a study focussed on the major human pathogen Staphylococcus aureus, which demonstrates the step changes such approaches can make to understanding microbial pathogenicity
The Mechanisms of Codon Reassignments in Mitochondrial Genetic Codes
Many cases of non-standard genetic codes are known in mitochondrial genomes.
We carry out analysis of phylogeny and codon usage of organisms for which the
complete mitochondrial genome is available, and we determine the most likely
mechanism for codon reassignment in each case. Reassignment events can be
classified according to the gain-loss framework. The gain represents the
appearance of a new tRNA for the reassigned codon or the change of an existing
tRNA such that it gains the ability to pair with the codon. The loss represents
the deletion of a tRNA or the change in a tRNA so that it no longer translates
the codon. One possible mechanism is Codon Disappearance, where the codon
disappears from the genome prior to the gain and loss events. In the
alternative mechanisms the codon does not disappear. In the Unassigned Codon
mechanism, the loss occurs first, whereas in the Ambiguous Intermediate
mechanism, the gain occurs first. Codon usage analysis gives clear evidence of
cases where the codon disappeared at the point of the reassignment and also
cases where it did not disappear. Codon disappearance is the probable
explanation for stop to sense reassignments and a small number of reassignments
of sense codons. However, the majority of sense to sense reassignments cannot
be explained by codon disappearance. In the latter cases, by analysis of the
presence or absence of tRNAs in the genome and of the changes in tRNA
sequences, it is sometimes possible to distinguish between the Unassigned Codon
and Ambiguous Intermediate mechanisms. We emphasize that not all reassignments
follow the same scenario and that it is necessary to consider the details of
each case carefully.Comment: 53 pages (45 pages, including 4 figures + 8 pages of supplementary
information). To appear in J.Mol.Evo
The stellar and sub-stellar IMF of simple and composite populations
The current knowledge on the stellar IMF is documented. It appears to become
top-heavy when the star-formation rate density surpasses about 0.1Msun/(yr
pc^3) on a pc scale and it may become increasingly bottom-heavy with increasing
metallicity and in increasingly massive early-type galaxies. It declines quite
steeply below about 0.07Msun with brown dwarfs (BDs) and very low mass stars
having their own IMF. The most massive star of mass mmax formed in an embedded
cluster with stellar mass Mecl correlates strongly with Mecl being a result of
gravitation-driven but resource-limited growth and fragmentation induced
starvation. There is no convincing evidence whatsoever that massive stars do
form in isolation. Various methods of discretising a stellar population are
introduced: optimal sampling leads to a mass distribution that perfectly
represents the exact form of the desired IMF and the mmax-to-Mecl relation,
while random sampling results in statistical variations of the shape of the
IMF. The observed mmax-to-Mecl correlation and the small spread of IMF
power-law indices together suggest that optimally sampling the IMF may be the
more realistic description of star formation than random sampling from a
universal IMF with a constant upper mass limit. Composite populations on galaxy
scales, which are formed from many pc scale star formation events, need to be
described by the integrated galactic IMF. This IGIMF varies systematically from
top-light to top-heavy in dependence of galaxy type and star formation rate,
with dramatic implications for theories of galaxy formation and evolution.Comment: 167 pages, 37 figures, 3 tables, published in Stellar Systems and
Galactic Structure, Vol.5, Springer. This revised version is consistent with
the published version and includes additional references and minor additions
to the text as well as a recomputed Table 1. ISBN 978-90-481-8817-
Microarray-Based Analysis of Differential Gene Expression between Infective and Noninfective Larvae of Strongyloides stercoralis
Strongyloides stercoralis is a soil-transmitted helminth that
affects an estimated 30–100 million people worldwide. Chronically infected
persons who are exposed to corticosteroids can develop disseminated disease, which
carries a high mortality (87–100%) if untreated. Despite this, little is
known about the fundamental biology of this parasite, including the features that
enable infection. We developed the first DNA microarray for this parasite and used it
to compare infective third-stage larvae (L3i) with non-infective first stage larvae
(L1). Using this method, we identified 935 differentially expressed genes. Functional
characterization of these genes revealed L3i biased expression of heat shock proteins
and genes with products that have previously been shown to be immunoreactive in
infected humans. Genes putatively involved in transcription were found to have L1
biased expression. Potential chemotherapeutic and vaccine targets such as
far-1, ucr 2.1 and hsp-90 were
identified for further study
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